深化教学改革修订教学计划培养高层次应用型人才   总被引：1，自引：0，他引：1  

吴亦凡 《怀化学院学报》2008,27(2):107-108

我校2007年全面修订本科培养计划,并以新的教学计划为依据,推进教学改革和建设,同时构建科学合理的实践教学体系,坚持产学研合作,培养具有国际化背景的高层次应用型人才。  相似文献   

姐姐是“菜农”     

吴亦凡 《生活教育》2010,(12):59-59

本期《生活作文》选录了江苏省如东县岔河小学学生的生活作文习作,并配发了朱慧老师在指导学生写作过程中的经验体会。希望能够有更多的老师和同学参与到我们这个栏目中来,欢迎广大老师热心推荐学生的佳作并且撰文阐述生活作文理念以及在作文教学中的实践体会。  相似文献   

Ex vivo expansions and transplantations of mouse bone marrow-derived hematopoietic stem/progenitor cells     

王金福  吴亦凡 《浙江大学学报(A卷英文版)》2004,5(2):157-163

To examine the effects of co-culture with bone marrow mesenchymal stem cells on expansion of hematopoietic stem/progenitor cells and the capacities of rapid neutrophil engraftment and hematopoietic reconstitution of the expanded cells, we expanded mononuclear cells (MNCs) and CD34⁺/c-kit⁺ cells from mouse bone marrow and transplanted the expanded cells into the irradiated mice. MNCs were isolated from mouse bone marrow and CD34⁺/c-kit⁺ cells were selected from MNCs by using MoFlo Cell Sorter. MNCs and CD34⁺/c-kit⁺ cells were co-cultured with mouse bone marrow-derived mesenchymal stem cells (MSCs) under a two-step expansion. The expanded cells were then transplanted into sublethally irradiated BDF1 mice. Results showed that the co-culture with MSCs resulted in expansions of median total nucleated cells, CD34⁺ cells, GM-CFC and HPP-CFC respectively by 10.8-, 4.8-, 65.9- and 38.8-fold for the mononuclear cell culture, and respectively by 76.1-, 2.9-, 71.7- and 51.8-fold for the CD34⁺/c-kit⁺ cell culture. The expanded cells could rapidly engraft in the sublethally irradiated mice and reconstitute their hematopoiesis. Co-cultures with MSCs in conjunction with two-step expansion increased expansions of total nucleated cells, GM-CFC and HPP-CFC, which led us to conclude MSCs may create favorable environment for expansions of hematopoietic stem/progenitor cells. The availability of increased numbers of expanded cells by the co-culture with MSCs may result in more rapid engraftment of neutrophils following infusion to transplant recipients. Project supported by NIH-Blood, Heart & Lung (National Institute of Health, USA, IR 01 4L70593-01) and Zhejiang Provincial Science Foundation (No. 011103397), China  相似文献