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Diversity of Gram negative bacteria antagonistic against major pathogens of rice from rice seed in the tropic environment 总被引：2，自引：0，他引：2
INTRODUCTIONRiceseedharborsadozenofplantpathogen icbacteria (Gotoetal.,1 988;Xieetal.,1 998) .Thebacterialpathogenscausearangeofricediseases,someverydestructivewhileothershavenoeffectonricecropgrowth .Inthisstudy ,wefoundalargenumberofbacteriaasso ciatedw… 相似文献
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浓缩接种法是传统的种子带菌检测方法,对水稻细菌性条斑病菌的可检测浓度为10~5个/ml左右,检测一批样品的速度为7～10d,但由于受到诸多环境因素和寄主的影响,该方法的稳定性和准确性较差。免疫放射分析法是一种新颖的超微量检测技术,由于它具有放射性同位素的灵敏性和血清学的特异性,用于细菌性条斑病菌的检测与前者相比具有许多优点,灵敏度高,可检测浓度为10~3个/ml;速度快,4h左右可得出结果;稳定性好,抗血清冻干品在4℃和-25℃下分别可保存70d和300d;可靠性强,与浓缩接种法的阴性符合率为100％,阳性符合率为63.04％。特别在低浓度下检测准确性更高。 相似文献
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谢关林 《浙江大学学报(A卷英文版)》2003,4(4):463-468
With the use of a seed washing technique, more than 4000 Gram negative bacteria were isolated by two improved isolation methods
from 446 batches of 1 kg rice seed samples obtained from 22 provinces in the Philippines. They were initially characterized
on the basis of colony morphology and results of biochemical and pathogenicity tests. Six hundred and fifty-two strains were
further identified by Biolog, from which 133 were selected for fatty acid methyl ester (FAME) analysis together with 80 standard
reference! strains. Six-teen species or types ofPseudomonas and 17 genera of non-pseudomonads were identified, more than one third of which have not been recorded in rice. The most
predominant species observed wereP. putida andP. fulva. About 17% of the strains ofPseudomonas and 2% of the non-pseudomonads were antagonistic to one or more fungal or bacterial pathogens of rice. Rice seed is an important
source of biological control agents.
Project supported by Belgium government and Asia Development Bank. Part of the experiments conducted at the University of
Gent, Belgium and IRRI during 1997–2000. 相似文献
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