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Use and effects of liver specific antigen in orthotopic liver transplantations were researched in this study. Group I: syngeneic
control (Wistar-to-Wistar); Group II: acute rejection (SD-to-Wistar); Group III: Thymic inoculation of SD rat LSA day 7 before
transplantation. The observation of common situation and survival time, rejection grades, NF-κB activity of splenocytes and
IL-2mRNA expression of grafted liver were used to analyze acute rejection severity and immune state of animals in different
groups. The common situation of group I was very well after transplantation and no signs of rejection were found. Recipients
of group II lost body weight progressively All dead within day 9 to day 13 posttransplantation; median survival time was 10.7
±0.51 days. It was an optimal acute rejection control. As for group III, 5 out of 6 recipients survived for a long time and
common situation was remarkably better than that of group II. Its rejection grades were significantly lower than that of group
II(P<0.05). NF-κB activity was only detected in group I at day 5 and day 7 after transplantation, whereas high activity of NF-κB
was detected at all time points in groupII and the low NF-κB activity detected in group III was significantly lower than that
of group II (P<0.05). No IL-2mRNA expression was detected at any time point in group I, whereas high level expression was detected at all
time points in group II and the low level expression only detected at day 3 in group III was significantly lower than that
of group II (P<0.05). Conclusion: LSA is an important transplantation antigen which is involved directly in the immunorejection of liver
transplantation. We report here for the first time that intrathymic inoculation of LSA can alleviate the rejection of liver
allotransplantation; and that grafts can survive for a long time thereby, thus leading to a novel way to achieve liver transplantation
immunotolerance.
Project (NO. 011106206) supported by the Science and Technology Bureau of Zhejiang Province Foundation 相似文献
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INTRODUCTIONAlthoughMHC (majorhistocompatibilitycomplex)antigenshavebeenextensivelystud ied ,theproblemofrejectioninlivertransplanta tionwasnotresolvedcompletely .Livertrans plantationarenotstrictforHLA(humanleuco cyteahtigen)matchingandbothgraftsandre cipientscansurviveforalongtimeafterimmu notherapy .Ontheotherhand ,givenHLAmul tiplelocimatchinginsomecases,thelikelihoodofgraftsdysfunctionandrejectionincreased ,whichsuggestedthatmatchingforHLAtypemayexertadualisticeffectonlivertransp… 相似文献
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Infusion of nonmyeloablative bone marrow alleviates acute rejection reaction in liver allotransplantation 总被引：1，自引：0，他引：1
Objective: To study the effect and implication of nonmyeloablative donor specific bone marrow (DSBM) infusion on the immunoreaction of liver allotransplantation. Methods: Orthotopic liver transplantation model was used in this study. Groups were set as follows: Group Ⅰ, syngeneic control (Wistar-to-Wistar); Group Ⅱ, acute rejection (SD-to-Wistar); Group Ⅲ, acute rejection treated with cyclosporine A (CsA) by intramuscular injection (SD-to-Wistar CsA); Group Ⅳ, bone marrow infusion at 7 d pretransplantation followed by short-term CsA treatment (SD-to-Wistar DSBM); Another group of short-term CsA treatment preoperatively without bone marrow infusion was also set as control. General characteristics and survival time were observed.Histological grades of rejection were determined by pathological examination. IL-2 and IFN-γ level in peripheral blood and donor liver were detected respectively by Enzyme-Linked Immuno-Sorbent Assay (ELISA) and Western blot. Chimerism of donor cells was measured by PCR for a male-specific marker (Y-chromosome-specific sequence, Sry). Results: No signs of rejection were found in Group Ⅰ. Acute rejection occurred in both Group Ⅱ and the short-term CsA treated group. All the recipients died at (9～15)d posttransplantation with a median survival time of (10.7±0.5) d and (11.2±2.4) d, respectively. Only mild rejection could be seen in Group Ⅲ. In Group Ⅳ, 4 out of 6 recipients had long-term survival (＞100 d), the histological grade of rejection was significantly lower than that of Group Ⅱ, so did the expression level of IL-2 and IFN-γ in both peripheral blood and grafted liver.Y-chromosome-specific sequence (Sry) of male SD rats could be detected in the bone marrow, spleen and thymus of female recipients at 15 d after bone marrow infusion. Conclusion: Mild preconditioning nonmyeloablative donor specific bone marrow infusion can enhance chimerism formation in recipients, alleviate the rejection of liver allotransplantation and prolong survival of liver allotransplantation. 相似文献
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