Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca2+ transport proteins and the effect of taurine on myocardial protection in rabbits     

黄先玫  朱卫华  康曼丽 《浙江大学学报(A卷英文版)》2003,4(1)

To investigate the effect of doxorubicin(DOX) on gene expression of the myocardial sarcoplasmic reticulum (SR)Ca2+ transport proteins and the mechanism of taurine(Tau) protecting cardiac muscle cells, 9 rabbits were injected with DOX , 8 rabbits with DOX and Tau, and 9 rabbits with normal saline. Cardiac function , concentration of calcium in cardiomyocytes (Myo[Ca2+]i), activity of SR Ca2+-ATPase(SERCA2a), level of SERCA2a mRNA and Ca2+ released channels(RYR2)mRNA were detected. The left ventricle tissues were observed by electron microscopy. The results showed that cardiac index, left ventricular systolic pressure, activity of SR Ca2+-ATPase and level of SERCA2a mRNA decreased , while Myo[Ca2+]i increased in DOX-treated rabbits. DOX could not affect the level of RYR2 mRNA. Tau intervention could alleviate the increase of left ventricular diastolic pressure, Myo[Ca2+]i and the decrease of SERCA2a mRNA induced by doxorubicin. The results suggested that downregulation of SERCA2a gene expression was an important mechanism of DOX-induced cardiomyopathy and that Tau could partially improve the heart function by reducing calcium overload and alleviating downregulation of SERCA2a mRNA.  相似文献   

阿霉素对兔血清NO含量和心肌细胞肌浆网Ca2+-ATP酶活性的影响     

黄先玫  余钟声 《科技通报》2000,16(5):392-395

为探讨阿霉素（ＡＤＲ）对心脏的慢性毒性作用,将新西兰纯种兔１８只随机分成ＡＤＲ组和对照组。ＡＤＲ组每周静脉注射一次生理盐水配制的ＡＤＲ,每公斤体重２ｍｇ,共８次。对照组仅注射生理盐水。于末次注射后３周,测量兔的心输出量（ＣＯ）、颈动脉压（ＢＰ）、劲动脉平均压（ＭＡＰ）、左心室收缩压（ＬＶＳＰ）和左心室舒张末压（ＬＶＥＤＰ）及心肌肌浆网（ＳＲ）的Ｃａ＾２＋－ＡＴＰ酶活性均明显低于对照组;ＬＶＥＤＰ和  相似文献   

阿霉素对兔血清NO含量和心肌细胞肌浆网Ca²⁺-ATP 酶活性的影响     

黄先玫  余钟声 《科技通报》2000

  相似文献   

Study on the effect of doxorubicin on expressions of genes encoding myocardial sarcoplasmic reticulum Ca~( 2+) transport proteins and the effect of taurine on myocardial protection in rabbits     

黄先玫  朱卫华  康曼丽 《Journal of Zhejiang University. Science. B》2003,(1)

ＩＮＴＲＯＤＵＣＴＩＯＮＤｏｘｏｒｕｂｉｃｉｎ(ＤＯＸ)ｉｓａｋｉｎｄｏｆａｎｔｈｒａｃｙ ｃｌｉｎｅｓａｎｔｉｂｉｏｔｉｃｓｗｈｏｓｅａｎｔｉｎｅｏｐｌａｓｔｉｃｓｐｅｃｔｒｕｍｉｓｂｒｏａｄａｎｄｅｆｆｅｃｔｉｓｓｔｒｏｎｇ.Ｉｔｈａｓｇａｉｎｅｄｗｉｄｅ ｓｐｒｅａｄｕｓｅｉｎｔｈｅｔｒｅａｔｍｅｎｔｏｆｃｈｉｌｄｈｏｏｄｌｅｕｋｅ ｍｉａａｎｄｓｏｌｉｄｔｕｍｏｒｓ.Ｈｏｗｅｖｅｒ,ｉｔｓｃｌｉｎｉｃａｌｕｓｅｉｓｌｉｍｉｔｅｄｂｙｉｔｓｃａｒｄｉｏｔｏｘｉｃｉｔｙ .Ｔｈｅｃａｌｃｉｕｍｏｖｅｒ…  相似文献