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1.

Introduction

Total 25-hydroxyvitamin D [25(OH)D] is the most reliable indicator of vitamin D status. In this study, we compared two automated immunoassay methods, the Abbott Architect 25-OH Vitamin D assay and the Roche Cobas Vitamin D total assay, with the liquid chromatography-tandem mass spectrometry (LC-MS/MS).

Materials and methods

One hundred venous blood samples were randomly selected from routine vitamin D tests. Two of the serum aliquots were analyzed at the Abbott Architect i2000 and the Roche Cobas 6000’s module e601 in our laboratory within the same day. The other serum aliquots were analyzed at the LC-MS/MS in different laboratory. Passing-Bablok regression analysis and Bland-Altman plot were used to compare methods. Inter-rater agreement was analyzed using kappa (κ) analysis.

Results

The Roche assay showed acceptable agreement with the LC-MS/MS based on Passing-Bablok analysis (intercept: -5.23 nmol/L, 95% CI: -8.73 to 0.19; slope: 0.97, 95% CI: 0.77 to 1.15). The Abbott assay showed proportional (slope: 0.77, 95% CI: 0.67 to 0.85) and constant differences (intercept: 17.08 nmol/L; 95% CI: 12.98 to 21.39). A mean bias of 15.1% was observed for the Abbott and a mean bias of -14.1% was observed for the Roche based on the Bland-Altman plots. We found strong to nearly perfect agreement in vitamin D status between the immunoassays and LC-MS/MS. (κ: 0.83 for Abbott, κ: 0.93 for Roche) using kappa analysis.

Conclusion

Both immunoassays demonstrated acceptable performance, but the Roche Cobas assay demonstrated better performance than the Abbott Architect in the studied samples.Key words: 25-Hydroxyvitamin D, chromatography, immunoassay, methods, tandem mass spectrometry  相似文献   
2.
The present article scrutinizes “the unique American vision of childhood” (UAVC) as a phenomenon undermining high academic expectations and good work ethics, and in turn, contributing to the generally low academic achievement of U.S. students compared to their counterparts in other advanced countries. It starts with a definition of UAVC, followed by a discussion of how influential it has been. The article goes on to state three reasons why UAVC is troublesome and misleading, especially in an era of global competition. Excuses devised by the proponents for UAVC’s adverse effects are also revealed. The article ends with recommendations for future research and a conclusion elaborating on the consequences of UAVC and the likelihood that other countries might adopt a similar mentality.  相似文献   
3.
Although libraries tend to see the integrated library system (ILS) as a very “library” system, it is actually a powerful inventory and patron management system that can be used much more broadly. At American University, the library has many campus partner collections that it has added to the library catalog. Some collections contain books and media, like traditional library holdings, and some are made up of audiovisual and computer equipment. This article describes the process of cataloging these collections, the challenges of conceiving the ILS as a campus-wide system, and the benefits that these partnerships have brought to the library.  相似文献   
4.
While adherence to medication remains a problematic area in the care of chronically ill elderly, an extended model that incorporates the interaction of studied variables remains a blank spot. Anchored on Ajzen's Theory of Planned Behavior, this article details our efforts to develop and test a model of medication adherence among Filipino elderly relative to their medication belief, follow-up visits, consultation satisfaction, memory task, trust with physician, perceived stress, memory strategies, social support, memory load, depression, length of time taking the medication, number of conditions, and self-efficacy with medication adherence. Structural equation modeling (SEM) was employed to study causalities among all parameters. With the participation of 325 older adults located in District IV, Sampaloc, Manila, data needed were gathered through a multiaspect questionnaire consisting of a robotfoto, a memory strategy usage measure, an eight-scale Morisky Medication Adherence Scale, a 15-item Geriatric Depression Scale, a General Self-efficacy Scale, a Consultation Satisfaction Questionnaire, and a Perceived Stress Scale. Data were analyzed using AMOS version 19. Results revealed that depression, trust with physician, and number of conditions affect adherence positively, while event-based memory, consultation satisfaction, memory load, and external memory strategy affect it otherwise. Some suggested variables were found to have no impact at all. As this study reveals, careful consideration should be given to multiple factors, and their interrelationship should be examined well. Because many factors can influence medication adherence behavior of the elderly, and because the reasons for their noncompliance are also varied, multifaceted solutions must be developed.  相似文献   
5.
For successful reading experiences in native and/or foreign/second language, individuals need to benefit from not only cognitive strategies but also metacognitive strategies. Although research found reading comprehension and performance increase following metacognitive trainings, such findings may not transfer into mainstream classrooms as easily for several reasons. This study, therefore, aimed to disseminate the phenomenon of teaching metacognition with an emphasis on teacher’s instrumental role during classroom learning. More specifically, it investigated language instructors’ metacognition and their self-reported competencies for teaching metacognition. It also examined whether and how self-reported competencies changed following a professional development (PD) module of teaching for metacognition. Utilising Metacognitive Awareness Inventory (MAI) and think aloud protocols for instructional planning, this study found most participants were either highly metacognitive or metacognitive individuals. It was also found that most participants were initially not knowledgeable about and/or competent in teaching metacognition. Following PD, highly metacognitive teachers developed authentic lesson plans manifesting metacognition instruction while metacognitive teachers adopted similar instructional designs presented during the PD. Besides, half of the participants appreciated teaching metacognitively following the PD. Finally, under the light of these findings, future research and policy adjustments were proposed.  相似文献   
6.
OBJECTIVE: To conceptualize the underlying causes of the medical neglect of children in a developing country and to provide suggestions for the management of neglect by pediatricians. METHODS: A case history of a 4-year-old boy from Turkey with neglect of the required treatment for acute lymphoblastic leukemia is used to examine the causes and management of medical neglect. Results: Although epidemiological studies on child neglect are lacking, this case exemplifies how in DEVELOPING countries, reasons for neglect or non-compliance with medical recommendations and the roles and actions taken by the health care and the social service systems may differ from western populations. Common to both western and developing countries, the characteristics of the child, family, and society may be reasons for medical neglect. However, cultural fatalistic beliefs profoundly present in the developing world may also contribute to the medical neglect of a child. Identification of the neglect, a comprehensive, multidisciplinary assessment emphasizing the strengths within the family and the society, and the determination of the pediatric team to act in the best interest of the child may result in resolution of the neglect even in circumstances where resources within systems are not sufficient. CONCLUSIONS: In developing countries, increased emphasis on child neglect, its prompt recognition and management within the pediatric profession as well as at a health care and social service system levels are needed to address this prevalent and potentially fatal child health problem.  相似文献   
7.
This research study aims to examine the effectiveness of a problem-based learning (PBL) on 9th grade students’ understanding of intermolecular forces (dipole–dipole forces, London dispersion forces and hydrogen bonding). The student’s alternate conceptions about intermolecular bonding and their beliefs about PBL were also measured. Seventy-eight 9th grade students were stratified by cognitive levels and then randomly assigned to experimental (PBL, 40 students) and control (lecture-style teaching, 38 students) groups. Following a preparatory lesson where activation and remediation of existing knowledge occur, a pre-test was given, and no significant difference was found between the two groups of students (p > .05). After the instruction was completed, a post-test and also a questionnaire related to the quality of the problem, the teacher’s role and group functioning were administered. Results from the post-test of both groups (p < .05) and questionnaire showed that PBL is affective on students’ achievement, remedying formation of alternate conceptions and also social skills.  相似文献   
8.
This article reports on a term's work with students in Initial Teacher Education (ITE) in which the adult classic text Silas Marner was studied in both written and film form ( Eliot, 1994 ; BBC/Fo, 1985 ). Through an extended consideration of the structures employed by different forms of narrative, students were invited to consider the knowledge and skills they brought to the development of their own response to different texts. Concurrently they were encouraged to consider the needs of their prospective pupils, as expert readers of moving image, and as novice readers of classic fiction. Crucial to the teaching and learning experience was the consideration of the different, expanding notions of literacy, including visual literacy, tele‐literacy and moving‐image literacy. A key consideration was the interrelationship between these, with narrative as the key link, and the exploration of how these literacies could be mutually supportive within the framework of ‘school’ literacy. The importance of paying explicit attention to the pedagogical practices that foster an engagement with and development of these different literacies was highlighted, as was the need to experience the reading of whole texts in order to foster the development of response.  相似文献   
9.
This grounded study examined the process of acclimatization or the process of changing perspectives of a person so as to get used to or feel “at home” among a select group of Filipino elderly in a nursing care facility. As institutionalization of the elderly is not typical in the Philippine context, varied responses were analyzed to understand the factors and the course that an elderly person undergoes over a set period of time to achieve acclimatization. Twenty elderly (n = 20) residents were purposively recruited and subjected to an in-depth interview that chronicled their experiences on their previous and present lives and on the process by which they were able to adjust to a relatively new setting. Preliminarily, a robotfoto was devised to obtain demographic data including visitation frequencies and familial relationships, and this was followed by an in-depth interview. Through the constant comparison method, an interesting model called the Hourglass of Acclimatization emerged. This model yielded two distinct phases contributing to successful acclimatization. One is the Conversion phase, or imbibing the main notion of transforming one's perspectives of him or herself and his or her environment; there is also the Immersion phase, which describes how an elderly involves him or herself completely into the life he or she is supposed to live. This emerged model can contribute to the development of nursing interventions focusing on elderly experiences in the entire course of relocation to a new environment other than what they call “home.”  相似文献   
10.
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