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Nam Cao Hoai Le Murat Gel Yonggang Zhu Jian Wang Helen Dacres Alisha Anderson Stephen C. Trowell 《Biomicrofluidics》2014,8(6)
Bioluminescence resonance energy transfer (BRET) is a form of Förster resonance energy transfer. BRET has been shown to support lower limits of detection than fluorescence resonance energy transfer (FRET) but, unlike FRET, has not been widely implemented on microfluidic devices for bioanalytical sensing. We recently reported a microscope-based microfluidic system for BRET-based biosensing, using a hybrid, high quantum-efficiency, form of BRET chemistry. This paper reports the first optical fiber-based system for BRET detection on a microfluidic chip, capable of quantifying photon emissions from the low quantum-efficiency BRET2 system. We investigated the effects of varying core diameter and numerical aperture of optical fibers, as well as varying microfluidic channel design and measurement conditions. We optimized the set-up in order to maximize photon counts and minimize the response time. The optimized conditions supported measurement of thrombin activity, with a limit of detection of 20 pM, which is lower than the microscope-based system and more than 20 times lower than concentrations reported to occur in plasma clots. 相似文献
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Federal legislation has driven and been receptive to the vision of a rigorous, relevant career and technical education (CTE) system integrated with academics and aligned across middle school, secondary school, and postsecondary education. This article uses a social policy analysis approach to trace the history of federal CTE policy throughout the 20th century and the beginning of the 21st century. Through this overview, we demonstrate how federal CTE policy has repeatedly evolved and responded to changing economic and social needs, while also incorporating policy flexibility that has led to variation in program implementation on the state and local levels. 相似文献
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This case study examines how a teacher defined ‘my kind of teaching’. We focus on a dramatic production of Charlie and the Chocolate Factory and frame the teacher’s discussion with Dewey’s significant moments of experience. Findings indicate the teacher shifted her role to a facilitator position, learned alongside her students, and connected her past experiences with acting. Implications point to expressive teaching moments as experiences in which teachers might extend state‐mandated curricula, connect with their personal lives, and find delight in teaching. 相似文献
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To determine if quizzes containing higher order thinking questions are related to critical thinking and test performance when utilised in conjunction with an immersion approach to instruction and effort-based grading, sections of an Educational Psychology course were assigned to one of three quizzing conditions. Quizzes contained factual multiple-choice questions, factual essay questions or essay items requiring higher order thinking. Critical thinking was measured with a pre-test–post-test design and the Watson–Glaser Critical Thinking Appraisal (Short Form). Classroom learning was assessed via multiple-choice and essay tests. Critical thinking increased equally across all sections. The section receiving higher order thinking quizzes performed significantly better than the other two sections on both the multiple-choice and essay portions of the classroom tests. The implications of these findings are discussed in the context of methodological approaches to encouraging critical thinking. 相似文献
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Access to primary and secondary education in Tanzania has drastically expanded in the past two decades. In response to this success, its ministry is now targeting improvements in educational quality through additional reform. Yet teacher absenteeism, physical abuse, overcrowding, lecture-based pedagogy and a system of accountability based primarily on state and district examinations centered on rote memorization impose great obstacles to students being prepared for future employment and further education. This study draws insights into how traditional teaching and learning methods in one rural village in Tanzania can be changed through examining a collaboratively created intensive after-school program that focused on three content areas and used a participatory action research approach centered on cooperative inquiry. The key elements of the participatory action research approach drawn upon were using a participatory model to create a community of co-learners, designing the curriculum collectively, students collecting data in their communities about identified problems, requiring all participants to contribute, student groups disseminating their findings through presentations and an ongoing support system for teacher development in applying a student-centered pedagogy. Students responded through increased school attendance, confidence, self-esteem and active engagement while teachers incorporated participatory methods of instruction in their classrooms. The factors accounting for the consensus of support by villagers and school and government leaders for the program include community and government buy-in, the creation of a community of learners, consistent follow-up support for teachers and reinforcement of expectations. 相似文献
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Sexual assault on college campuses has been a longstanding problem in the USA. Unfortunately, relatively little has been done to look at the characteristics of these attacks and how they might relate to knowledge of sexual assault policies, participation in sex education courses, beliefs toward sexual violence, and willingness to intervene if an attempted assault is observed. In response to this need, data were collected from college students from four universities in the state of Mississippi. A request to participate in an online, anonymous questionnaire was sent through email and listservs, and a total of 4,994 undergraduate students completed the survey. A significant relationship was found between men’s sexual attitudes and understanding of legal terms and their willingness to intervene if a sexual assault occurred; less legal knowledge and higher rape myth attitudes reduced their probability of being willing to intervene. Conversely, students who had received education at college on how to prevent a sexual assault from occurring were significantly to report being willing to intervene. 相似文献
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