排序方式： 共有18条查询结果，搜索用时 15 毫秒
1.
Arnold Chen Royal Wang Candace R. S. Bever Siyuan Xing Bruce D. Hammock Tingrui Pan 《Biomicrofluidics》2014,8(6)
The emerging technologies on mobile-based diagnosis and bioanalytical detection have enabled powerful laboratory assays such as enzyme-linked immunosorbent assay (ELISA) to be conducted in field-use lab-on-a-chip devices. In this paper, we present a low-cost universal serial bus (USB)-interfaced mobile platform to perform microfluidic ELISA operations in detecting the presence and concentrations of BDE-47 (2,2′,4,4′-tetrabromodiphenyl ether), an environmental contaminant found in our food supply with adverse health impact. Our point-of-care diagnostic device utilizes flexible interdigitated carbon black electrodes to convert electric current into a microfluidic pump via gas bubble expansion during electrolytic reaction. The micropump receives power from a mobile phone and transports BDE-47 analytes through the microfluidic device conducting competitive ELISA. Using variable domain of heavy chain antibodies (commonly referred to as single domain antibodies or Nanobodies), the proposed device is sensitive for a BDE-47 concentration range of 10−3–104 μg/l, with a comparable performance to that uses a standard competitive ELISA protocol. It is anticipated that the potential impact in mobile detection of health and environmental contaminants will prove beneficial to our community and low-resource environments. 相似文献
2.
3.
4.
5.
Arapera Royal Tangaere Stuart McNaughton 《International Journal of Early Years Education》2003,2(1):23-40
Processes of generalising bilingual expertise from Te Kohanga Reo (an indigenous preschool for the revitalisation of Maori language) to home are analysed. Observations at the preschool and home settings revealed several features. The active and strategic role of the child in transferring expertise was evident. So too were the presence of complementary activities at home, sensitive responding to generalised language and shared cultural commitments. These processes were present in a range of activities. The results are discussed in terms of theoretical approaches to generalisation and the effectiveness of indigenous language intervention programmes. 相似文献
6.
7.
Kylie A. Royal Damian Farrow Iñigo Mujika Shona L. Halson David Pyne Bruce Abernethy 《Journal of sports sciences》2013,31(8):807-815
Abstract The aim of this study was to assess the effects of fatigue on decision making and goal shooting skill in water polo. Fourteen junior elite male players (age 17.2 ± 0.5 years; mass 84.2 ± 7.6 kg; height 1.85 ± 0.05 m) completed four sets of eight repetitions of an approximately 18 s maximal water polo specific drill. Progressively declining rest ratios for each successive set of the drill were employed to induce increasing fatigue and reflect the demands of match-play. A video-based temporally occluded decision-making task (verbalized response to various tactical situations) or goal shooting skill test (qualitative and quantitative analysis of goal shooting) was performed after each set. Heart rate, rating of perceived exertion (RPE) and blood lactate concentration were recorded. Heart rate (159 ± 12, 168 ± 13, 176 ± 12, 181 ± 12 Deats min?1; P < 0.001) and RPE (13.1 ± 2.2, 15.5 ± 1.7, 17.3 ± 1.6, 19.1 ± 1.1; P < 0.001) increased with declining rest ratios. At very high fatigue, decision-making accuracy was 18.0 ± 21.8% better than at low fatigue (P = 0.008). Shooting accuracy and velocity were unaffected by incremental fatigue; however, skill proficiency (technique) decreased by 43 ± 24% between the pre-test and high-fatigue conditions (P < 0.001). In conclusion, incremental increases in fatigue differentially influenced decision making (improved) relative to the technical performance (declined), accuracy and speed of the ball (unchanged) of a water polo goal shot. 相似文献
8.
9.
10.