Comparative study of Anaplasma parasites in tick carrying buffaloes and cattle     

Rajput ZI  Hu SH  Arijo AG  Habib M  Khalid M 《Journal of Zhejiang University. Science. B》2005,6(11):1057-1062

INTRODUCTION The world population of buffaloes(Bubalus bubalis)has been estimated at over172million head,more than97%(167.6million)of which are in Asia and the Pacific region,mainly in India(97.7million)and Pakistan(25.5million)(FAO,2004).About98%of buffaloes in the region are raised by small farmers owning less than two hectares of land and less than five buffaloes(Mudgal,1992).In general,the water buffalo is regarded as more productive,healthier and more useful than the cow,especiall…  相似文献   

Study of cellulolytic soil fungi and two nova species and new medium     

Khalid M  Yang WJ  Kishwar N  Rajput ZI  Arijo AG 《Journal of Zhejiang University. Science. B》2006,7(6):459-466

This study is aimed at identifying and determining the percentage of occurrence frequency of cellulose decomposing soil fungi. The soil samples were inoculated into culture plates prepared in Sabouraud medium under sterilized conditions and incubated at 30 ℃ for 4 to 7 d. The identified fungal species were incubated in self-designed cellulose medium for testing their cellulolytic ability. Forty-two species, including2 nova species, representing sixteen genera showed growth and sporulation in the cellulose medium. Most of the isolated species were from genus Aspergillus and Penicillium. Aspergillus niger and Mucor hiemalis showed highest occurrence frequency （45% and 36% respectively）, as these species were collected from about 80% of soil samples. Being agar free and cheaper, the new fungal medium designed showed results equivalent to Sabouraud medium.  相似文献   

Adjuvant effects of saponins on animal immune responses   总被引：2，自引：0，他引：2  

Rajput ZI  Hu SH  Xiao CW  Arijo AG 《Journal of Zhejiang University. Science. B》2007,8(3):153-161

Vaccines require optimal adjuvants including immunopotentiator and delivery systems to offer long term protection from infectious diseases in animals and man. Initially it was believed that adjuvants are responsible for promoting strong and sustainable antibody responses. Now it has been shown that adjuvants influence the isotype and avidity of antibody and also affect the properties of cell-mediated immunity. Mostly oil emulsions, lipopolysaccharides, polymers, saponins, liposomes, cytokines, ISCOMs (immunostimulating complexes), Freund’s complete adjuvant, Freund’s incomplete adjuvant, alums, bacterial toxins etc., are common adjuvants under investigation. Saponin based adjuvants have the ability to stimulate the cell mediated immune system as well as to enhance antibody production and have the advantage that only a low dose is needed for adjuvant activity. In the present study the importance of adjuvants, their role and the effect of saponin in immune system is reviewed.  相似文献   

Improvement of the efficacy of influenza vaccination （H5N1） in chicken by using extract of Cochinchina momordica seed （ECMS）   总被引：1，自引：0，他引：1  

Rajput ZI  Xiao CW  Hu SH  Arijo AG  Soomro NM 《Journal of Zhejiang University. Science. B》2007,8(5):331-337

Seeds of a Chinese traditional medicine plant, Cochinchina momordica were used in the present study for the improvement of influenza vaccine （HSN 1） in chicken. Crude extraction from Cochinchina momordica seed （ECMS） was obtained by ethanol extraction method. In experiment No. 1, two weeks old chickens were immunized with influenza vaccine （HSN1） alone or combined with ECMS （5, 10, 20, 40 and 80 μg/dose）. Serum IgG antibody levels （by ELISA） as well as effects on dally weight gain were measured on 0, 7, 14 and 28th day after immunization. Results revealed that all ECMS groups numerically increased the antibody levels while 10 and 20 μg/dose groups significantly （P〈0.05） enhanced total IgG antibody on day 28, when compared with control. Average daily weight gain was also significantly higher in 20 μg/dose ECMS group. Adjuvant effect was also confirmed in experiment No. 2 when chickens were immunized with 20 μg/dose ECMS and antibody titer was measured through hemagglutination inhibition （HI）. It is concluded that ECMS has potential to improve the immune responses and deserve further study as an adjuvant.  相似文献   

Importance of ticks and their chemical and immunological control in livestock     

Rajput ZI  Hu SH  Chen WJ  Arijo AG  Xiao CW 《Journal of Zhejiang University. Science. B》2006,7(11):912-921

The medical and economic importance of ticks has long been recognized due to their ability to transmit diseases to humans and animals. Ticks cause great economic losses to livestock, and adversely affect livestock hosts in several ways. Loss of blood is a direct effect of ticks acting as potential vector for haemo-protozoa and helminth parasites. Blood sucking by large numbers of ticks causes reduction in live weight and anemia among domestic animals, while their bites also reduce the quality of hides. However, major losses caused by ticks are due to their ability to transmit protozoan, rickettsial and viral diseases of livestock, which are of great economic importance world-wide. There are quite a few methods for controlling ticks, but every method has certain shortcomings. The present review is focused on ticks importance and their control.  相似文献