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Palani Damotharan Anguchamy Veeruraj Muthuvel Arumugam Thangavel Balasubramanian 《Indian journal of clinical biochemistry : IJCB》2016,31(1):57-67
This study is designed to isolate and purify a novel anti-clotting protein component from the venom of Enhydrina schistosa, and explore its biochemical and biological activities. The active protein was purified from the venom of E. schistosa by ion-exchange chromatography using DEAE-cellulose. The venom protein was tested by various parameters such as, proteolytic, haemolytic, phospholipase and anti-coagulant activities. 80 % purity was obtained in the final stage of purification and the purity level of venom was revealed as a single protein band of about 44 kDa in SDS-polyacrylamide electrophoresis under reducing conditions. The results showed that the Potent hemolytic activity was observed against cow, goat, chicken and human (A, B and O positive) erythrocytes. Furthermore, the clotting assays showed that the venom of E. schistosa significantly prolonged in activated partial thromboplastin time, thrombin time, and prothrombin time. Venomous enzymes which hydrolyzed casein and gelatin substrate were found in this venom protein. Gelatinolytic activity was optimal at pH 5–9 and 1H NMR analysis of purified venom was the base line information for the structural determination. These results suggested that the E. schistosa venom holds good promise for the development of novel lead compounds for pharmacological applications in near future. 相似文献
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Wieslaw L. Nowinski Arumugam Thirunavuukarasuu Anand Ananthasubramaniam Beng Choon Chua Guoyu Qian Natalia G. Nowinska Yevgen Marchenko Ihar Volkau 《Anatomical sciences education》2009,2(5):244-252
Preparation of tests and student's assessment by the instructor are time consuming. We address these two tasks in neuroanatomy education by employing a digital media application with a three‐dimensional (3D), interactive, fully segmented, and labeled brain atlas. The anatomical and vascular models in the atlas are linked to Terminologia Anatomica. Because the cerebral models are fully segmented and labeled, our approach enables automatic and random atlas‐derived generation of questions to test location and naming of cerebral structures. This is done in four steps: test individualization by the instructor, test taking by the students at their convenience, automatic student assessment by the application, and communication of the individual assessment to the instructor. A computer‐based application with an interactive 3D atlas and a preliminary mobile‐based application were developed to realize this approach. The application works in two test modes: instructor and student. In the instructor mode, the instructor customizes the test by setting the scope of testing and student performance criteria, which takes a few seconds. In the student mode, the student is tested and automatically assessed. Self‐testing is also feasible at any time and pace. Our approach is automatic both with respect to test generation and student assessment. It is also objective, rapid, and customizable. We believe that this approach is novel from computer‐based, mobile‐based, and atlas‐assisted standpoints. Anat Sci Educ 2:244–252, 2009. © 2009 American Association of Anatomists. 相似文献
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Srinivasa Nageswara Rao G Prema G Priya G Arumugam SB Kirthivasan V Saibabu R Cherian KM 《Indian journal of clinical biochemistry : IJCB》2011,26(1):22-27
There is a rising trend in the prevalence of insulin resistance among obese, overweight children and adolescents. The serum
insulin and its correlation with biochemical, clinical and anthropometric parameters were evaluated in 185 children and adolescents
(59 control, 52 obese, 49 overweight, 25 congenital heart disease) of age group 10–17 years. The levels of serum insulin were
measured by ELISA. Serum insulin levels were found to be significantly increased in children who were obese, overweight and
had congenital heart disease, than controls. Serum insulin levels positively correlated with BMI, WHR, and serum C-peptide,
serum leptin, total cholesterol, triglycerides, LDL-cholesterol, systolic and diastolic blood pressure. Fasting glucose levels
were found to be negatively correlated with serum insulin levels. HDL-cholesterol levels were non-significant among the study
groups. We identified nine obese children (five girls and four boys) with the features of metabolic syndrome and 69% of obese
and overweight children were identified with insulin resistance. Insulin resistance was strongly associated with metabolic
syndrome and its components, especially with central obesity and hypertriglyceridemia. 相似文献
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