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Jang K Xu Y Tanaka Y Sato K Mawatari K Konno T Ishihara K Kitamori T 《Biomicrofluidics》2010,4(3):32208
Recently, interest in single cell analysis has increased because of its potential for improving our understanding of cellular processes. Single cell operation and attachment is indispensable to realize this task. In this paper, we employed a simple and direct method for single-cell attachment and culture in a closed microchannel. The microchannel surface was modified by applying a nonbiofouling polymer, 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer, and a nitrobenzyl photocleavable linker. Using ultraviolet (UV) light irradiation, the MPC polymer was selectively removed by a photochemical reaction that adjusted the cell adherence inside the microchannel. To obtain the desired single endothelial cell patterning in the microchannel, cell-adhesive regions were controlled by use of round photomasks with diameters of 10, 20, 30, or 50 μm. Single-cell adherence patterns were formed after 12 h of incubation, only when 20 and 30 μm photomasks were used, and the proportions of adherent and nonadherent cells among the entire UV-illuminated areas were 21.3%±0.3% and 7.9%±0.3%, respectively. The frequency of single-cell adherence in the case of the 20 μm photomask was 2.7 times greater than that in the case of the 30 μm photomask. We found that the 20 μm photomask was optimal for the formation of single-cell adherence patterns in the microchannel. This technique can be a powerful tool for analyzing environmental factors like cell-surface and cell-extracellular matrix contact. 相似文献
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Kihoon Jang Yo Tanaka Jun Wakabayashi Reina Ishii Kae Sato Kazuma Mawatari Mats Nilsson Takehiko Kitamori 《Biomicrofluidics》2012,6(4)
Demand for analysis of rare cells such as circulating tumor cells in blood at the single molecule level has recently grown. For this purpose, several cell separation methods based on antibody-coated micropillars have been developed (e.g., Nagrath et al., Nature 450, 1235–1239 (2007)). However, it is difficult to ensure capture of targeted cells by these methods because capture depends on the probability of cell-micropillar collisions. We developed a new structure that actively exploits cellular flexibility for more efficient capture of a small number of cells in a target area. The depth of the sandwiching channel was slightly smaller than the diameter of the cells to ensure contact with the channel wall. For cell selection, we used anti-epithelial cell adhesion molecule antibodies, which specifically bind epithelial cells. First, we demonstrated cell capture with human promyelocytic leukemia (HL-60) cells, which are relatively homogeneous in size; in situ single molecule analysis was verified by our rolling circle amplification (RCA) method. Then, we used breast cancer cells (SK-BR-3) in blood, and demonstrated selective capture and cancer marker (HER2) detection by RCA. Cell capture by antibody-coated microchannels was greater than with negative control cells (RPMI-1788 lymphocytes) and non-coated microchannels. This system can be used to analyze small numbers of target cells in large quantities of mixed samples. 相似文献
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Educators from a variety of disciplines include the concept of transgender and multiple gender identities in course curricula. The ‘T’ (denoting the specific inclusion of transgender) in the popular acronym LGBT (lesbian, gay, bisexual, transgender) often is given less pedagogical attention than is sexual orientation, and the transgender concept often is taught in the context of sexual orientation. An experimental study was conducted to determine if a brief film intervention would yield differences in knowledge of the transgender concept. Individuals were randomly assigned to one of three conditions. Conditions varied to include no exposure to the concept of being transgender, a 14-minute news documentary about the transgender concept, or a 28-minute news documentary about the transgender concept. Results supported hypotheses that a brief film intervention would produce higher accuracy of transgender knowledge. In addition, and supporting the contact hypothesis, participants with a transgender friend reported less transphobia and more empathy for transgender individuals. 相似文献
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The old American concept of “melting pot” may no longer be the correct conceptual framework to use when dealing with the influx of new immigrants into the work force. It may be best neither to treat nor to expect everyone to be the same but rather to consider and to accommodate ethnic diversity. Harmonization may provide more effective management than homogenization. Problems can arise when words, examples, and relationships between trainers, trainees, and managers are contrary to the cultural norms and expectations of the employees. Managers and trainers can improve human performance by changing their standard operating procedures to include an understanding of the implication of ethnicity in the training environment. Although there is little research on the new labor force and few tested strategies, some earlier work addressed the preparation of employees of multinational corporations for work abroad. This paper explores ethnic differences and potential problems resulting from a new labor force and discusses some potential approaches to interethnic training based on investigations in sociological and communications research. 相似文献
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