One step antibody-mediated isolation and patterning of multiple cell types in microfluidic devices     

Danny Bavli  Elishai Ezra  Daniel Kitsberg  Margarita Vosk-Artzi  Shashi K. Murthy  Yaakov Nahmias 《Biomicrofluidics》2016,10(2)

Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.  相似文献   

Cinema Demand in Spain: A Cointegration Analysis   总被引：4，自引：3，他引：1  

VÍctor FernÁndez Blanco  JosÉ F. BaÑos Pino 《Journal of Cultural Economics》1997,21(1):57-75

In this paper we explain the decline in cinema attendance in Spain since 1968. We use a cointegration analysis to estimate an individual demand function for cinema, with annual data and in terms of average attendance per inhabitant. We find that cinema is a luxury good and that its demand is elastic with respect to its price. We also show that television acts as a deterrent with regard to cinema attendance. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

“Theorizing teacher mobility”: a critical review of literature     

Robert Vagi  Margarita Pivovarova 《Teachers and Teaching》2017,23(7):781-793

In this critical review of literature, we summarize the major theoretical frameworks that have been used to study teacher mobility. In total we identified 40 teacher mobility studies that met our inclusion criteria. We conclude that relatively few theoretical frameworks have been used to study teacher mobility and those that have been used are lacking in several key areas. Specifically, none of the current teacher mobility theories are able to adequately account for the many teacher- and school-level factors that likely influence teachers’ professional decisions. Finally, we suggest a theoretical framework from the organizational psychology literature that can address the shortcomings of theories identified in this review.  相似文献   

Identification of gifted students with learning disabilities in a Response‐to‐Intervention era     

Franci Crepeau‐Hobson  Margarita Bianco 《Psychology in the schools》2011,48(2):102-109

The identification of children who are twice‐exceptional—those who are gifted and have concomitant learning disabilities (LDs)—has historically posed a number of challenges for school psychologists and other school personnel. With the reauthorization of the Individuals With Disabilities Education Act and the shift to the use of a Response‐to‐Intervention (RtI) model to identify students with LDs, the task of identifying those who are twice exceptional is even more daunting. This article proposes an integrated model for the identification of gifted children with LDs that blends standardized assessment methods with practices consistent with RtI. This balanced approach brings together the best of both worlds to more accurately identify twice‐exceptional students and better meet their educational needs. © 2010 Wiley Periodicals, Inc.  相似文献   

From The Editors’ Desk     

Margarita Machado-Casas  Enrique G. Murillo Jr. 《Journal of Latinos & Education》2014,13(2):83-84

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Challenge to feminist philosophy     

Margarita Levin 《Academic Questions》1988,1(4):48-53

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Adaptation and evaluation of a learning analytics dashboard to improve academic support at three Latin American universities     

Julio Guerra  Margarita Ortiz-Rojas  Miguel Angel Zúñiga-Prieto  Eliana Scheihing  Alberto Jiménez  Tom Broos  Tinne De Laet  Katrien Verbert 《British journal of educational technology : journal of the Council for Educational Technology》2020,51(4):973-1001

Despite the success of academic advising dashboards in several higher educational institutions (HEI), these dashboards are still under-explored in Latin American HEI's. To close this gap, three different Latin American universities adapted an existing advising dashboard, originally deployed at the KU Leuven to their own context. In all three cases, the context was the main ruling factor to these adaptations. In this paper, we describe these adaptions using a framework that focuses on four different elements of the context: Objectives, Stakeholders, Key moment and Interactions. Evaluation of the adapted dashboards in the three different Latin American universities is conducted through pilots. This evaluation shows the value of the dashboard approach in different contexts in terms of satisfaction, usefulness and impact in academic decision-making and advising tasks. The main contribution of this paper is the systematic reporting of the adaptations to an academic advising dashboard and showing the value of an academic advising dashboard on academic decision-making and advising tasks.  相似文献   

Description of Environmental Factors in Schools: Lessons from a Study in North–West Spain     

Julia Crespo Comesa?a  Margarita Pino Juste 《International Review of Education/Internationale Zeitschrift für Erziehungswissenschaft/Revue internationale l'éducation》2007,24(1):205-218

The objective of this study is to offer a view on the different environmental factors that affect health (sound, light, colour, temperature) in the design, planning and organization of school premises. To achieve this, the authors first outline the problems leading to unhealthy situations. They subsequently analyse all the building and planning factors behind those problems based on a study carried out in the Autonomous Community of Galicia, in the north west of Spain. The study took as its subject all secondary-level education centres built in Galicia since the Spanish Basic Act on the Educational System (LOGSE) was passed by the Spanish Parliament in 1990. To conclude, the article offers a number of guidelines to be taken into account when designing a school environment.  相似文献   

Science Classroom Interactions and Academic Language Use with English Learners     

Tiberio Garza  Margarita Huerta  Tracy G. Spies  Rafael Lara-Alecio  Beverly J. Irby  Fuhui Tong 《International Journal of Science and Mathematics Education》2018,16(8):1499-1519

Academic language is critical for English learners (ELs) to succeed in the science classroom. This study analyzed the instructional practices centered around academic language taking place in eight, fifth-grade classrooms during a grant-funded project. Observational data collected through a classroom observation instrument were analyzed to describe and compare the instructional practices occurring in treatment and control classrooms when teachers and students used academic language. Our findings show both treatment and control classrooms using academic language during the course of the intervention study, but the instruction looked markedly different in each setting. These differences provide valuable insight for practitioners and researchers concerned with the academic language development of ELs in science classrooms.  相似文献   

AILEM Programme: a long‐term intervention to promote literacy learning in low‐performing primary schools in Chile     

Malva Villalón Bravo Corresponding author  Margarita Silva  Tonia Razmilic  Stanley L. Swartz 《Early Years: An International Journal of Research and Development》2005,25(2):97-111

After one year of implementation, this paper describes a programme designed to support literacy learning in low performing schools from poor sectors in Santiago, Chile. School‐based intensive training on theory and practice of a literacy learning framework for classroom instruction and long‐term support were used to impact the achievement of students from disadvantaged families in 16 kindergarten classrooms and 16 1st‐grade classrooms in nine primary schools. Preliminary results showed an improvement in learning achievement positively related to level of implementation of the literacy instruction framework in the classrooms and teachers' perception of an improvement in their knowledge and classroom practices. Although findings from this study cannot support definite conclusions, because of the lack of experimental conditions, they provide relevant information for future experimental studies on how to meet the literacy learning needs of children from disadvantaged backgrounds.  相似文献