排序方式： 共有8条查询结果，搜索用时 15 毫秒
1
1.
J. Pramanik A. N. Lodam C. M. Badole M. V. R. Reddy K. R. Patond B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2000,15(1):22-28
Trichloroacetic acid (TCA) solubilized and DEAE fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (ES) antigen viz., Mtb EST DE1 and affinity purified goat antibodies to the TCA solubilized ES antigen
(Mtb EST) were explored in detecting tubercular antibody and antigen respectively in sera of bone and joint tuberculosis by
indirect and sandwich ELISA. Out of total 36 bone & joint tuberculosis cases, tubercular antibody was detected by indirect
ELISA in 30 patients (sensitivity 83%), while circulating tubercular antigen was detected by sandwich ELISA in 27 patients
(sensitivity 75%). Out of 34 non tubercular disease control cases, 10 patients showed positive reaction for antibody while
only 4 patients showed positive reaction for antigen. In another group of 34 healthy subjects who were screened, 4 individuals
showed positive reaction for tubercular antibody and 2 cases for antigen. This study shows that antigen detection assay using
affinity purified anti Mtb EST antigen antibody is superior with overall specificity of 91% as compared to antibody detection
assay with 75% specificity in bone & joint tuberculosis. 相似文献
2.
Sanyal N Pramanik SK Pal R Chowdhury A 《Journal of Zhejiang University. Science. B》2006,7(3):202-208
Two sulfonylurea herbicides, metsulfuron methyl （Ally 20 WP） and chlorimuron ethyl （Classic 25 WP） were evaluated for their dissipation bchaviour in alluvial, coastal saline and laterite soils under laboratory incubated condition at 60% water holding capacity of soils and 30 ℃ temperature was maintained. In field study herbicides were applied twice for the control of grasses, annual and perennials broad leaves weeds and sedges in rice, wheat and soybean to find out the residual fate of both the herbicides on different matrices of respective crops after harvest. Extraction and clean up methodologies for the herbicides were standardized and subsequently analyzed by HPLC. The study revealed that the half-lives of metsulfuron methyl and chlorimuron ethyl ranged from 10.75 to 13.94 d irrespective of soils and doses applied. Field trials with rice, wheat and soybean also revealed that these two herbicides could safely be recommended for application as no residues were detected in the harvest samples. 相似文献
3.
4.
Vietnam universities have experienced remarkable changes brought about by their internationalization policies. The switch to English as a medium of instruction (EMI) for some academic programs was one of these critical changes. Literature has reported numerous issues related to EMI, including inadequate language proficiency of teaching staff. This paper looks at a qualitative research study on how a government university from Vietnam employs different strategies to enhance teachers’ English proficiency. The study reveals that the introduction of new supporting systems, assessment bodies, recruitment criteria and institutional strategies on training, monitoring and motivation have created cultural change within the teacher community. This cultural change, which includes elements such as self-directed learning, peer learning, professionalism, and ‘open-to-change’ attitudes, has been perceived by both leaders and teachers to be conducive to teachers’ language learning. The findings presented in this paper seek to contribute to the formulation or adjustment of policies related to educational reforms, such as curriculum reform, teacher recruitment and teacher professional development in non-English-speaking countries. 相似文献
5.
6.
A. N. Lodam J. Pramanik M. V. R. Reddy P. Narang B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》1997,12(1):71-77
Tuberculosis is emerging as a major public health problem in developing and developed world. Early and precise diagnosis is
of prime importance in successful control of infection. Indirect ELISA with penicillinase as marker was developed using purifiedM. tuberculosis excretory-secretory (EST-DE1) antigen for detecting IgG antibodies in pulmonary tuberculosis. The assay System gave a overall sensitivity of 82% for both
smear positive and smear negative pulmonary tuberculosis cases with a specificity of 84%. The positive and negative predictive
values were 75% and 88% respectivaly. Further studies with EST-DE1 antigen revealed that, it contains two of the active antigen fractions of Mtb EST antigen i.e. Mtb EST-4 (56–68 KDa) and
Mtb EST-6 (37–45 KDa), as demonstrated by inhibition ELISA. Reactivity with monoclonal antibodies HGT 3a showed the presence
of 38 KDa molecule in EST-DE1 antigen. 相似文献
8.
1