Diversity of Gram negative bacteria antagonistic against major pathogens of rice from rice seed in the tropic environment     

谢关林 《浙江大学学报(A卷英文版)》2003,4(4):463-468

With the use of a seed washing technique, more than 4000 Gram negative bacteria were isolated by two improved isolation methods from 446 batches of 1 kg rice seed samples obtained from 22 provinces in the Philippines. They were initially characterized on the basis of colony morphology and results of biochemical and pathogenicity tests. Six hundred and fifty-two strains were further identified by Biolog, from which 133 were selected for fatty acid methyl ester (FAME) analysis together with 80 standard reference! strains. Six-teen species or types ofPseudomonas and 17 genera of non-pseudomonads were identified, more than one third of which have not been recorded in rice. The most predominant species observed wereP. putida andP. fulva. About 17% of the strains ofPseudomonas and 2% of the non-pseudomonads were antagonistic to one or more fungal or bacterial pathogens of rice. Rice seed is an important source of biological control agents. Project supported by Belgium government and Asia Development Bank. Part of the experiments conducted at the University of Gent, Belgium and IRRI during 1997–2000.  相似文献