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Vashist SK Kaur I Bajpai RP Bharadwaj LM Tewari R Raiteri R 《Journal of Zhejiang University. Science. B》2006,7(9):683-685
We report an important observation that the surface conductivity of antibody layer immobilized on polylysine-coated glass
substrate decreases upon the formation of complex with their specific antigens. This change in conductivity has been observed
for both monoclonal and polyclonal antibodies. The conductance of monoclonal mouse IgG immobilized on polylysine-coated glass
substrate changed from 1.02×10−8 Ω−1 to 1.41×10−11 Ω−1 at 10 V when complex is formed due to the specific biomolecular interactions with rabbit anti-mouse IgG F(ab′)2. Similar behavior was observed when the same set up was tested in two clinical assays: (1) anti-Leishmania antigen polyclonal
antibodies taken from Kala Azar positive patient serum interacting with Leishmania promastigote antigen, and (2) anti-p21
polyclonal antibodies interacting with p21 antigen. The proposed concept can represent a new immunodiagnostic technique and
may have wide ranging applications in biosensors and nanobiotechnology too. 相似文献
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VASHIST Sandeep Kumar KAUR Inderpreet BAJPAI Ram Prakash BHARADWAJ Lalit Mohan TEWARI Rupinder RAITERI Roberto 《Journal of Zhejiang University. Science. B》2006,7(9)
Electrical conductivity in biomolecules and other large polymeric systems has become an important topic of current research. Schlag et al.(2000a; 2000b) demonstrated that charge migration in proteins is highly efficient although the mechanistic origin is still debated and largely unknown even though various models have been proposed by Weinkauf et al.(1995; 1996; 1997). According to them, the charge transfer between amino acids takes place by hole hopping between local sites of lowest ionizati… 相似文献
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Nihal G. Maremanda Kislay Roy Rupinder K. Kanwar Vidyarani Shyamsundar Vijayalakshmi Ramshankar Arvind Krishnamurthy Subramanian Krishnakumar Jagat R. Kanwar 《Biomicrofluidics》2015,9(5)
The role of circulating tumor cells (CTCs) in disease diagnosis, prognosis, monitoring of the therapeutic efficacy, and clinical decision making is immense and has attracted tremendous focus in the last decade. We designed and fabricated simple, flat channel microfluidic devices polydimethylsiloxane (PDMS based) functionalized with locked nucleic acid (LNA) modified aptamers (targeting epithelial cell adhesion molecule (EpCAM) and nucleolin expression) for quick and efficient capture of CTCs and cancer cells. With optimized flow rates (10 μl/min), it was revealed that the aptamer modified devices offered reusability for up to six times while retaining optimal capture efficiency (>90%) and specificity. High capture sensitivity (92%) and specificity (100%) was observed in whole blood samples spiked with Caco-2 cells (10–100 cells/ml). Analysis of blood samples obtained from 25 head and neck cancer patients on the EpCAM LNA aptamer functionalized chip revealed that an average count of 5 ± 3 CTCs/ml of blood were captured from 22/25 samples (88%). EpCAM intracellular domain (EpICD) immunohistochemistry on 9 oral squamous cell carcinomas showed the EpICD positivity in the tumor cells, confirming the EpCAM expression in CTCs from head and neck cancers. These microfluidic devices also maintained viability for in vitro culture and characterization. Use of LNA modified aptamers provided added benefits in terms of cost effectiveness due to increased reusability and sustainability of the devices. Our results present a robust, quick, and efficient CTC capture platform with the use of simple PDMS based devices that are easy to fabricate at low cost and have an immense potential in cancer diagnosis, prognosis, and therapeutic planning. 相似文献
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