Analysis of bulked segregants to identify molecular markers linked with cocoon weight and cocoon shell weight in the silkworm Bombyx mori L     

徐孟奎  陈玉银 《浙江大学学报(A卷英文版)》2002,3(3)

Two silkworm strains viz, B20 A (high cocoon shell ratio) and C.Nichi (low cocoon shell ratio) were sib mated for 10 generations to determine the homozygosis. Both bulked segregant analysis(BSA) and near isogenic lines (NIL) studies were done to identify the RFLP markers closely linked to cocoon shell parameters. Three hundred and fifty-two random clones were identified as the low copy number sequence and used for identification of Restriction Fragment Length Polymorphic (RFLP) marker linked to cocoon weight and cocoon shell character. In the bulk segregant analysis, DNA from the parents (B20 A, C.Nichi), F1 and F2 progeny of high shell ratio (HSR) and low shell ratio (LSR) were screened for hybridization with the random clones. Polymorphic banding pattern achieved through southern hybridization with different probes indicated the probable correlation of polymorphism with high and low cocoon shell character which are possible landmarks in identifying the putative marker(s) for the cocoon shell character. Out of the 100 probes tried with parents, F1, F2 and their bulks, 10 probes were found to be closely linked to cocoon shell characters.  相似文献   

家蚕体内的类胡萝卜素及其结合蛋白     

靳远祥  姜永煌  陈玉银  徐孟奎 《科技通报》2003,19(6):516-520

类胡萝卜素是一类有相似结构的生物色素的总称，在生物体内有重要的生理功能．家蚕从食下的桑叶中获取大量的类胡萝卜素，并广泛分布于中肠、血淋巴、丝腺等组织中．类胡萝卜素对家蚕幼虫的生长发育有一定的影响，尤其是对幼虫和成虫的趋光性和视觉感光性的产生有重要的作用．同其它的生物相似，家蚕体内的类胡萝卜素也是和蛋白质结合在一起形成复合物，类胡萝卜素结合蛋白在维护类胡萝卜素的稳定、体内贮存及转运上都起着重要的作用．  相似文献   

家蚕从基因组到蛋白质组的研究   总被引：1，自引：0，他引：1  

颜新培  徐孟奎 《科技通报》2004,20(3):252-257

家蚕基因组计划实施以来,用DNA限制性片段长度多态性(RFLP)、随机扩增片段多态性(RAPD)、扩增片段长度多态性(AFLP)、微卫星序列长度多态性(SSR)等多种分子标记技术进行了家蚕基因组多态性分析,构建了家蚕分子连锁图谱;开展了家蚕基因组序列测定和功能基因的研究.在后基因组时代,蛋白质组研究是基因功能研究的重要内容.家蚕蛋白质组研究虽然刚刚起步,但已确立了家蚕蛋白质样品制备方法,利用双向电泳技术和图像分析技术进行了家蚕功能蛋白质研究.该文对家蚕基因组和蛋白质组的研究进展进行了综述.  相似文献