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目的 研究ICSI治疗前无精子症患者Y染色体微缺失发生率及微缺失类型,探讨精子生成障碍与Y染色体微缺失的关系.方法 应用PCR技术,对63例ICSI前行诊断性睾丸活检的无精子症患者进行Y染色体15个序列标记位点的微缺失检测.结果 63例无精子症患者中发现12例有Y染色体微缺失,缺失率为19.1%.其中10例患者的缺失发生在AZFc/DAZ区域,缺失率为15.8%;2例患者发生AZFb、AZFc双重缺失,缺失率为3.2%,AZFa无缺失病例.结论 Y染色体微缺失是引起男性无精子症的重要原因之一.采用PCR技术进行微缺失检测是一种非常有效的方法.对无精子症患者进行ICSI治疗前,有必要进行Y染色体的微缺失筛查. 相似文献
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旨在探讨模板制备方法对单细胞 PCR准确性的影响 ,采用 KOH/ DTT和液氮冻融煮沸两种不同方法裂解单个淋巴细胞 ,行 DMD基因外显子 17、19、4 4、4 5、4 8五重巢式 PCR.用 KOH/DTT法裂解的细胞扩增成功率为 98% ( 147/ 150 ) ,失败率为 2 % ( 3/ 150 ) ;用液氮冻融煮沸法裂解的细胞扩增成功率为 78% ( 117/ 150 ) ,失败率为 2 2 % ( 33/ 150 ) ,差异有显著性 (χ2 =2 8.4 ,P <0 .0 1) .结果表明单细胞 PCR模板制备与 PCR扩增失败有密切关系 ,选用合适的细胞裂解方法可提高单细胞 PCR的准确性 相似文献
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Objective: To investigate the factors that might influence the success of an embryo freezing and thawing program. Method:
The relationship between the pregnancy rate in 73 cycles of embryo, freezing and thawing program and the following factors
was analyzed: maternal age,E
2 level at the time of HCG trigger, embryo storage time, number of thawed embryos transferred, presence of sponsoring embryos
and intact embryos. And the survival rate of thawed embryos with different morphology, cell stage and storage time was evaluated.
Result: Transfer with three or more than three thawed embryos resulted in pregnancy rates of 38.5% and 35.7%, respectively,
compared with 5.3% for transfer of fewer than three embryos. The presence of sponsoring embryos and intact embryos significantly
increases pregnancy rate in embryo freezing and thawing program. No other factor examined had any effect on pregnancy outcome.
The survival rate of good morphology embryos was higher than poor ones, but was not influenced by cell stage and storage time.
Conclusion: Embryo morphology before freezing, number of thawed embryos transferred and the presence of intact embryos are
important to the outcome of embryo freezing and thawing program. 相似文献
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为探讨体外受精－胚胎移植（IVT－ET）过程中影响临床妊娠率的因素，对248个常规IVF－ET周期进行了分析，研究了夫妇年龄、穿刺卵泡数、获取卵母细胞数、卵母细胞成熟卵比率、卵母细胞空卵比率、精液精子质量和数量、授精前孵育时间、授精精子密度、受精率、多原核孕卵生成率、卵裂率、移植胚胎数目、胚胎累积评分及移植过程评分和临床妊娠之间的关系。结果表明，临床妊娠率与母方年龄呈显著性负相关（P＜0．05），与移植胚胎数目、胚胎累积评分和移植过程评分呈显著性正相关（P＜0．05）。说明母方年龄、移植胚胎数目、胚胎累积评分和移植过程评分是影响临床妊娠的重要因素。 相似文献
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Objective: Embryonic chromosomal abnormality is one of the main reasons for in vitro fertilization (IVF) failure. This study aimed at evaluating the value of Fluorescence in-situ Hybridization (FISH)-based Preimplantation Genetic Diagnosis (PGD) in screening for embryonic chromosomal abnormality to increase the successful rate of IVF. Method: Ten couples, four with high risk of chromosomal abnormality and six infertile couples, underwent FISH-based PGD during IVF procedure. At day 3, one or two blastomeres were aspirated from each embryo. Biopsied blastomeres were examined using FISH analysis to screen out embryos with chromosomal abnormalities. At day 4, embryos without detectable chromosomal abnormality were transferred to the mother bodies as in regular IVF. Results: Among 54 embryos screened using FISH-based PGD, 30 embryos were detected to have chromosomal abnormalities. The 24 healthy embryos were implanted, resulting in four clinical pregnancies, two of which led to successful normal birth of two 相似文献
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Objective: Embryonic chromosomal abnormality is one of the main reasons for in vitro fertilization (IVF)failure. This study aimed at evaluating the value of Fluorescence in-situ Hybridization (FISH)-based Preimplantation Genetic Diagnosis (PGD) in screening for embryonic chromosomal abnormality to increase the successful rate of IVF. Method:Ten couples, four with high risk of chromosomal abnormality and six infertile couples, underwent FISH-based PGD during IVF procedure. At day 3, one or two blastomeres were aspirated from each embryo. Biopsied blastomeres were examined using FISH analysis to screen out embryos with chromosomal abnormalities. At day 4, embryos without detectable chromosomal abnormality were transferred to the mother bodies as in regular IVF. Results: Among 54 embryos screened using FISH-based PGD, 30 embryos were detected to have chromosomal abnormalities. The 24 healthy embryos were implanted,resulting in four clinical pregnancies, two of which led to successful normal birth of two healthy babies; one to ongoing pregnancy during the writing of this article; and one to ectopic pregnancy. Conclusion: FISH-based PGD is an effective method for detecting embryonic chromosomal abnormality, which is one of the common causes of spontaneous miscarriages and chromosomally unbalanced offsprings. 相似文献
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