Place-based education from three perspectives     

Jan Cincera  Barbora Valesova  Sarka Krepelkova  Petra Simonova  Roman Kroufek 《Environmental Education Research》2019,25(10):1510-1523

Abstract

This article examines the perception of the Czech place-based education program School for Sustainable Development from the perspectives of the participating students, teachers, and representatives of the local municipalities. The study is based on an analysis of paired or group interviews with 47 students, 7 teachers, and 6 representatives. The findings show that while the teachers perceived the process of the students’ involvement in planning and conducting a community-based project as highly participative, the students’ opportunities to shape the process through making their own decisions were rather limited and the teachers kept control over important parts of the process to themselves. While the cooperation of the schools with their respective municipalities was appreciated by most of the respondents, several barriers have been identified. Participation in the project has likely increased the teachers’ self-effectiveness, developed the students’ interpersonal competence, and improved the atmosphere at the schools involved.

Abbreviations: PBE: place-based education; ENEC: European Network for Environmental Citizenship; STEM: Science, Technology, Engineering and Mathematics; SSD: The School for Sustainable Development.  相似文献   

Development of a magnetic immunosorbent for on-chip preconcentration of amyloid β isoforms: Representatives of Alzheimer's disease biomarkers     

Svobodova Z  Reza Mohamadi M  Jankovicova B  Esselmann H  Verpillot R  Otto M  Taverna M  Wiltfang J  Viovy JL  Bilkova Z 《Biomicrofluidics》2012,6(2):24126-2412612

Determination of amyloid β (Aβ) isoforms and in particular the proportion of the Aβ 1-42 isoform in cerebrospinal fluid (CSF) of patients suspected of Alzheimer's disease might help in early diagnosis and treatment of that illness. Due to the low concentration of Aβ peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (μIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Aβ antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the proper beads and anti-Aβ antibodies, as well as optimizing the immobilization technique and μIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Aβ IS for μIP and specific capture of 5 Aβ peptides under optimized conditions using various subsequent analytical methods, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), capillary electrophoresis, microchip electrophoresis, and immunoblotting. Synthetic Aβ peptides samples prepared in buffer and spiked in human CSF were analyzed. Finally, on-chip immunoprecipitation of Aβ peptides in human CSF sample was performed.  相似文献   

Effective measures to foster girls’ interest in secondary computer science education     

Happe  Lucia  Buhnova  Barbora  Koziolek  Anne  Wagner  Ingo 《Education and Information Technologies》2021,26(3):2811-2829

The interest of girls in computing drops early during primary and secondary education, with minimal recovery in later education stages. In combination with the growing shortage of qualified computer science personnel, this is becoming a major issue, and also a target of numerous studies that examine measures, interventions, and strategies to boost girls’ commitment to computing. Yet, the results of existing studies are difficult to navigate, and hence are being very rarely employed in classrooms. In this paper, we summarize the existing body of knowledge on the effective interventions to recruit and retain girls in computer science education, intending to equip educators with a comprehensive and easy-to-navigate map of interventions recommended in the existing literature. To this end, we perform an aggregated umbrella literature review of 11 existing reviews on the topic, together accumulating joined knowledge from over 800 publications, and formulate the findings in a map of 22 concrete interventions structured in six groups according to their phase and purpose.

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Development of a magnetic immunosorbent for on-chip preconcentration of amyloid �� isoforms: Representatives of Alzheimer��s disease biomarkers     

Zuzana Svobodova  Mohamad Reza Mohamadi  Barbora Jankovicova  Hermann Esselmann  Romain Verpillot  Markus Otto  Myriam Taverna  Jens Wiltfang  Jean-Louis Viovy  Zuzana Bilkova 《Biomicrofluidics》2012,6(2):024126-024126-12

Determination of amyloid β (Aβ) isoforms and in particular the proportion of the Aβ 1-42 isoform in cerebrospinal fluid (CSF) of patients suspected of Alzheimer’s disease might help in early diagnosis and treatment of that illness. Due to the low concentration of Aβ peptides in biological fluids, a preconcentration step prior to the detection step is often necessary. This study utilized on-chip immunoprecipitation, known as micro-immunoprecipitation (μIP). The technique uses an immunosorbent (IS) consisting of magnetic beads coated with specific anti-Aβ antibodies organized into an affinity microcolumn by a magnetic field. Our goal was to thoroughly describe the critical steps in developing the IS, such as selecting the proper beads and anti-Aβ antibodies, as well as optimizing the immobilization technique and μIP protocol. The latter includes selecting optimal elution conditions. Furthermore, we demonstrate the efficiency of anti-Aβ IS for μIP and specific capture of 5 Aβ peptides under optimized conditions using various subsequent analytical methods, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), capillary electrophoresis, microchip electrophoresis, and immunoblotting. Synthetic Aβ peptides samples prepared in buffer and spiked in human CSF were analyzed. Finally, on-chip immunoprecipitation of Aβ peptides in human CSF sample was performed.  相似文献