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Saba Khan Roshan Alam Moinuddin Asif Ali 《Indian journal of clinical biochemistry : IJCB》2009,24(2):123-130
The present study was designed to probe the possible role of singlet oxygen and superoxide anion radical modified DNA in the
etiopathogenesis of Systemic lupus erythematosus. These species were generated by the exposure of riboflavin to 365 nm UV
light. Modified DNA showed single strand breaks, hyperchromicity at 260nm and decrease in Tm. The modified DNA induced high
titer antibodies in experimental animals. The antibodies showed reactivity with various nucleic acid polymers, a property
commonly associated with Systemic lupus erythematosus anti-DNA autoantibodies. Systemic lupus erythematosus sera showed preferential
binding of modified DNA over native DNA in direct binding and competitive binding solid phase immunoassays and band shift
assays. The results suggest for the possible involvement of the singlet- superoxide modified DNA as a potential trigger for
anti- DNA autoantibody production in SLE and thus in the etiopathogenesis of the disease. 相似文献
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Reactive oxygen species (ROS) are cytotoxic at higher concentration resulting in cell death, mutations, chromosomal aberrations
or carcinogenesis. In this study DNA was modified by singlet oxygen and superoxide anion radicals generated by illumination
of riboflavin under 365 nm UV-light. The modified DNA induced high titre antibodies in experimental animals. In enzyme immunoassay,
serum antibodies from cancer patients (n = 34) showed a higher recognition of the modified DNA, as compared to the native form. This was further confirmed by the
gel-shift assay. Immune IgG were used as a probe to detect oxidative lesions in the DNA of cancer patients. DNA isolated from
lymphocytes of cancer patients proved to be an appreciable inhibitor of the experimentally induced antibodies against the
ROS-DNA. This indicates the presence of oxidative lesions in the DNA obtained from cancer patients. The results show that
ROS induced oxidative damage to DNA in cancer patients generate neo-epitopes that are alien for the immune system, resulting in autoantibody formation. 相似文献
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A. D. Deepak Moinuddin S. A. Siddiqui A. Ali 《Indian journal of clinical biochemistry : IJCB》2000,15(1):6-10
In this study we have modified DNA by exposing it to ultraviolet light in the presence of hydrogen peroxide. The modified
DNA was probed for binding to the antibodies present in the sera of patients suffering from various types of cancer. Higher
recognition of modified DNA, as compared to native DNA, by antibodies from cancer patients has got far reaching significance. 相似文献
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