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Microfluidic devices have been established as useful platforms for cell culture for a broad range of applications, but challenges associated with controlling gradients of oxygen and other soluble factors and hemodynamic shear forces in small, confined channels have emerged. For instance, simple microfluidic constructs comprising a single cell culture compartment in a dynamic flow condition must handle tradeoffs between sustaining oxygen delivery and limiting hemodynamic shear forces imparted to the cells. These tradeoffs present significant difficulties in the culture of mesenchymal stem cells (MSCs), where shear is known to regulate signaling, proliferation, and expression. Several approaches designed to shield cells in microfluidic devices from excessive shear while maintaining sufficient oxygen concentrations and transport have been reported. Here we present the relationship between oxygen transport and shear in a "membrane bilayer" microfluidic device, in which soluble factors are delivered to a cell population by means of flow through a proximate channel separated from the culture channel by a membrane. We present an analytical model that describes the characteristics of this device and its ability to independently modulate oxygen delivery and hemodynamic shear imparted to the cultured cells. This bilayer configuration provides a more uniform oxygen concentration profile that is possible in a single-channel system, and it enables independent tuning of oxygen transport and shear parameters to meet requirements for MSCs and other cells known to be sensitive to hemodynamic shear stresses. 相似文献
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Sonika Gupta Niraj Shende Swati Banerjee Satish Kumar M. V. R. Reddy Bhaskar C. Harinath 《Indian journal of clinical biochemistry : IJCB》2002,17(1):5-8
Tuberculosis remains major health problem in India and developing countries Immunodiagnosis has important role in screening,
diagnosis and management of tuberculosis. SEVA TB ES-31 antigen has shown potential in detecting tuberculous IgG antibody
in earlier studies from our laboratory. In the present study we have analysedSEVA TB ES-31 antigen specific immunoglobulinsIgM, IgA and IgG in clinically and bacteriologically confirmed pulmonary tuberculosis cases to determine the usefulness of specific immunoglobulin
class in the diagnosis of patients attending the hospital.
Of the 30 cases of pulmonary tuberculosis 25 (83.3%) were positive for IgG, 19 (63.3%) for IgM and 16 (53.3%) for IgA. On
combining IgG and IgM positivity, sensitivity was increased to 93.3%. While combining IgG and IgA positivity, sensitivity
increased to 90%. However specificity was decreased to 66.6% and 70% for both of these combinations respectively. It could
be envisaged from this study that IgG antibody detection against ES-31 antigen showed acceptable sensitivity (83.3%) and specificity
(86.6%) compared to IgM or IgA alone or in combination. When immune responses were analysed according to degree of sputum
positivity, IgG response was observed to be predominant in all grades, compared to IgM or IgA antibody. The addition of IgM
or IgA as an adjunct test increases the sensitivity but at the cost of specificity. Hence the detection of IgG alone is more
useful compared to IgM or IgA assay, in detecting tuberculosis disease cases coming to the hospital. 相似文献
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Namdev S. Togre Priyanka S. Bhoj Vishal K. Khatri Aditya Tarnekar Kalyan Goswami Moreshwar R. Shende M. V. R. Reddy 《Indian journal of clinical biochemistry : IJCB》2018,33(3):282-289
Helminthic infections lead to the release of various molecules which play an important role in modulation of the host immune system. Such filarial proteins with immunomodulatory potential can be used for therapeutic purpose in inflammatory and immune mediated diseases. In the present study, we have explored the prophylactic effect of filarial SXP–RAL family protein of Wuchereria bancrofti i.e. rWbL2 protein in DSS induced inflammatory ulcerative colitis in a mouse model. Prior treatment of rWbL2, followed by induction of colitis, showed significantly reduced disease severity as indicated by the decreased disease manifestations and improved macroscopic and microscopic inflammation. This preventive effect was found to be associated with increased release of anti-inflammatory cytokine IL-10 and decreased release of proinflammatory cytokines IFN-γ, TNF-α, IL-6 and IL-17 by the splenocytes of treated mice. From this study, it can be envisaged that pretreatment with filarial protein, rWbL2, can prevent the establishment of ulcerative colitis in BALB/c mice. The underlying immunological mechanism may involve the up-regulation of Th2 immune response with down-regulation of Th1 response. 相似文献
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Niraj Prasad 《Resonance》2018,23(6):703-712
We discuss an application of interpolation of curves to extract fuel efficiency information of cars. Input data is taken from the mileage meter in the dashboard display of cars equipped with such a facility. This is also an application of harmonic mean. 相似文献
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课程建设与改革是适应经济发展和知识经济社会的客观要求,也是培养具有应用与创新能力的工程技术人才的必要手段。为此,以能力培养为宗旨,依托动力工程与工程热物理传统优势学科及多门能源动力类优秀课程,在结合教学实际情况的基础上,展开换热器原理与设计课程教学大纲、内容、方法、手段的改革,使得这门实践性很强的专业必修课具有鲜明的特色和创新。 相似文献
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Informed Systems promotes using information to learn during systems design and within designed systems, to advance coworker capability and further organizational capacity. In this spirit, an Informed Systems Capacity Bridge Model is presented, which connects individual information use and collective workplace learning. The associated Information Curation and Knowledge Management Conceptual Map presents requisite elements of an information-focused and technology-enabled workplace ecosystem. Concluding reflections place information experience in the foreground to enhance ‘what works well’ for ‘learning together’ through ‘designing together’, to catalyze workplace learning, activate systems thinking, animate reflective dialog, and catalyze organizational inquiry. 相似文献
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铸造行业广泛采用混砂机来制造树脂自硬砂用于造型或制芯.由于铸造环境恶劣,在设备电器选型和改造时,必须避免因外围抗干扰措施不利而使可编程控制器控制系统的可靠性降低,同时通过编制合理完善的程序来保证设备功能的完备性和运行的可靠性.本文的技术方案是根据设备参数选用可编程控制器对混砂机进行技术改造,优化混砂机的电气控制系统,设备实现了4年零故障运行,取得了很好的经济效益. 相似文献
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Swati Banerjee Sonika Gupta Niraj Shende Satish Kumar Bhaskar C. Harinath 《Indian journal of clinical biochemistry : IJCB》2003,18(2):48-53
Serodiagnosis by ELISA has been widely explored over the years, in the diagnosis of tuberculosis. Two ELISA systems were evaluated
for detection of mycobacterial antibodies in pulmonary and extra pulmonary tuberculosis. The two test assays explored were
ERBA LISA (TB IgG) test (Anda Biologicals) which uses A60 antigen complex found in the cytosol of typical and atypical mycobacteria,
and SEVA TB (IgG) ELISA, which uses a 31 kDa, glycoprotein antigen purified fromM. tb H37Ra culture filtrate. Sera from 98 proven tuberculosis [pulmonary TB (48), tuberculous lymphadenopathy (30), tuberculous meningitis
(15) & genitourinary TB (5)] were studied along with 32 healthy controls. The overall positivity obtained using ERBA LISA
(TB IgG) test and SEVA TB (IgG) ELISA test was 72.9% and 91.6% in pulmonary tuberculosis, 43.3% and 76.6% in tuberculous lymphadenopathy
respectively. The sensitivity of ERBA LISA test in tuberculous meningitis and genito-urinary TB was significantly low (26.6%
& 40% respectively) compared to sensitivity obtained using SEVA TB ELISA (86.6% & 60% respectively) with overall specificity
of 60% and 87.5%. Thus SEVA TB IgG ELISA test was found to be more sensitive than ERBA LISA in detecting IgG antibodies in
tuberculous sera, in particular in extra pulmonary tuberculosis cases. 相似文献
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Niraj Kumar 《Journal of Higher Education Policy & Management》2016,38(6):676-689
The objectives of this paper were to explore the existing status of agricultural higher education in India, application of marketing concepts in similar institutions and to find the most appropriate marketing concept to make agricultural higher education more competitive. Extensive searches of relevant agricultural education, business management and higher educational data bases were made. It was found that agricultural higher education in India is stagnant and is facing an identity crisis among its stakeholders. Marketing theories and concepts which are now being applied in higher education across the globe have not been thought of in the context of agriculture education. A review of the literature suggested that considering the objectives, the multiplicity of stakeholders and the nature of public-funded agricultural education, marketing orientation would be most appropriate. Based on the experiences of similar institutions, the paper suggests a three-level strategy for the implementation of marketing orientation in institutions of agricultural higher education. 相似文献
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