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1.
T. Malati G. Rajani Kumari P. V. L. N. Murthy Ch. Ram Reddy B. Surya Prakash 《Indian journal of clinical biochemistry : IJCB》2006,21(1):34-40
Prostate Specific Antigen (PSA) has emerged as the most applicable and important tumor marker for carcinoma prostate. In the
present study PSA was determined in serum of healthy subjects, patients of benign prostate hypertrophy (BPH) and Carcinoma
Prostate (Ca−P) to evaluate its diagnostic efficiency in day to day management of prostate cancer patients and in differentiating
patients of early prostate cancer from those with BPH. Receiver operating characteristic curve (ROC) revealed 2 ng/ml and
10 ng/ml cut off serum PSA level for BPH and untreated carcinoma prostate patients (Ca−P). An extremely significant increase
(P<0.0001) was observed in mean PSA concentration in BPH patients and adenocarcinoma prostate patients when compared to healthy
males. Clinical relevance of PSA was highlighted by a case study of cancer patient prior to any therapy till death. 相似文献
2.
Chandrawati Kumari Bijo Varughese Siddarth Ramji Seema Kapoor 《Indian journal of clinical biochemistry : IJCB》2016,31(4):414-422
Pre analytical process of extraction for accurate detection of organic acids is a crucial step in diagnosis of organic acidemias by GCMS analysis. This process is accomplished either by solid phase extraction (SPE) or by liquid–liquid extraction (LLE). Both extraction procedures are used in different metabolic laboratories all over the world. In this study we compared these two extraction procedures in respect of precision, accuracy, percent recovery of metabolites, number of metabolites isolated, time and cost in a resource constraint setup. We observed that the mean recovery from SPE was 84.1 % and by LLE it was 77.4 % (p value <0.05). Moreover, the average number of metabolites isolated by SPE and LLE was 161.8 ± 18.6 and 140.1 ± 20.4 respectively. The processing cost of LLE was economical. In a cost constraint setting using LLE may be the practical option if used for organic acid analysis. 相似文献
3.
Poonam Gautam Taruna Madan W. N. Gade P. Usha Sarma 《Indian journal of clinical biochemistry : IJCB》2006,21(2):12-19
Allergenic/antigenic proteins are known to induce Type I and Type III hypersensitivity reactions leading to allergic bronchopulmonary
aspergillosis (ABPA) in immunocompetent host. The common structural features or intrinsic properties of the allergens/antigens
leading to allergenicity in a host are not well understood. In the current report, comparative analysis of proteins on two
dimensional gel electrophoresis (2D-3) and specific IgE immunoblots ofA. fumigatus secretory proteins (1st, 2nd and 3rd week culture filtrate proteins) was carried out. We observed a total of 159 proteins in 1st, 2nd and 3rd week culture filtrates ofA. fumigatus. Specific IgE immunoreactivity was observed in 75 proteins with different intensity. Third week culture filtrate showed maximum
number of proteins, 142, and specific IgE immunoreactive proteins, 65. MALDI-TOF analysis resulted in putative identification
of two allergens as hypothetical protein YBL057c fromSaccharomyces cereviseae and unnamed protein product fromDebaryomyces hansenii (similar to IPF14568 ofCandida albicans). Identification of a repertoire of specific IgE immunoreactive proteins will facilitate the studies on structure-function
relationship of these proteins relevant for diagnosis and pathogenesis. 相似文献
4.
Dash P Pati S Mangaraj M Sahu PK Mohapatra PC 《Indian journal of clinical biochemistry : IJCB》2011,26(2):182-186
Now a days measurement of molecular forms of PSA has gained importance in clinical practice. Several studies have demonstrated
the production of PSA in female tissues, such as breast. The present piece of work has been undertaken with an objective to
estimate the relative proportion of the molecular forms of PSA in serum along with serum testosterone in benign and malignant
breast tumor cases and to analyze their association with the severity of the disease process 34 malignant and 26 benign breast
disease cases along with 33 healthy controls of same age group were enrolled in this study for evaluation. Serum testosterone
was measured by ELISA, whereas serum total PSA (TPSA) and free PSA (FPSA) were estimated by electrochemiluminescence immunoassay.
A significant rise of fasting plasma glucose along with prominent dyslipidemia was observed in breast tumor cases. Marked
rise in serum testosterone as well as TPSA and FPSA was documented in both benign and malignant breast tumor cases. Serum
testosterone revealed a significant positive association with both TPSA and FPSA pointing towards an etiological association
between them. However, surgical removal of tumor mass resulted in a marked decline of presurgical value of both TPSA and FPSA
with a non-significant fall in serum testosterone revealing tumor tissue as the source of FPSA and TPSA. Thus, estimation
of PSA provides prognostic information that may assist in future treatment. 相似文献
5.
Kavita Shalia Sudha Savant Vijaya A. Haldankar Tulip Nandu Poonam Pawar Siddhi Divekar V. K. Shah Purvi Bhatt 《Indian journal of clinical biochemistry : IJCB》2012,27(1):74-82
To analyse the association of high sensitivity C-reactive (hsCRP) protein levels and −717A/G single nucleotide polymorphism
of CRP with acute myocardial infarction (AMI) in the Indian population. Study population included 100 MI cases wherein 32
patients had experienced previous MI (MI-Group-1), 68 MI cases were recruited at presentation (MI-Group-2) and equal number
of age and gender matched healthy individuals. hsCRP levels were determined by ELISA and genotyping of −717A/G was carried
out by polymerase chain reaction-based restriction digestion method. The −717A/G genotypes did not influence hsCRP level and
their distribution did not differ between groups. However, in the present study hsCRP demonstrated significant correlation
with BMI in controls of both the genders and with triglycerides in females of AMI at presentation who otherwise are with low
risk profile. Identifying traditional risk factors associated with inflammation may help in controlling the acute event. 相似文献
6.
T. Malati G. Rajani Kumari I. Dinakar 《Indian journal of clinical biochemistry : IJCB》1995,10(2):72-76
Humoral immune response against PPD derived A60 antigen was evaluated by quantification of serum A60 antibodies in thrity healthy adults not exposed to tuberculosis (Group 1), in twenty seven healthy adults exposed to tuberculosis patients i.e. staff working in wards of tuberculosis hospital for one to thirty years (group 2), in twenty five pulmonary tuberculosis patients admitted to the Institute for Chest Diseases, Hyderabad (Group 3) and in sixty neurotuberculosis patients admitted to Neurosurgery department of our institute (Group 4). Highly significant elevation of A60 antibodies was observed in pulmonary tuberculosis patients (p<0.01) compared to healthy adult groups. A significant elevation in serum was also observed in case of neurotuberculosis group compared to both healthy groups (p<0.01). A test on A60 antibodies in serum gavv a sensitivity of 100%, specificity of 96.6%, positive predictive value of 81% and negative predictive value of 100% for pulmonary tuberculosis, whereas a sensitivity of 58%, positive predictive value of 79% and negative predictive value of 75% were noted for neurotuberculosis patients. Results of A60 antibodies in ten cerebrospinal fluids (CSF) obtained from non tuberculosis patients and thirty two CSF from patients of neurotuberculosis did not show significant elevation of antibodies. However the ninetyfive percentile value of CSF A60 antibodies was higher in neurotuberculosis (7.4 U/ml) group compared to nontuberculous group (3.8 U/ml) and the test showed a good positive predictive value (83%), very low negative predictive value (25%) and low sensitivity (63%). Serum A60 antibody assay appears to be a good serological marker available today for pulmonary tuberculosis and a supportive marker for neurotuberculosis. 相似文献
7.
T. Malati G. Rajani Kumari B. Yadagiri 《Indian journal of clinical biochemistry : IJCB》2001,16(2):224-233
Ovarian cancer is the fifth leading cause of death in women. The incidence of this malignancy increases in women over the
age of 40. The overall five years survival is less than 30%, as most women present with advanced stage disease. Until recently,
detection of early stage ovarian cancer has been difficult since it is usually nonpalpable and asymptomatic. The definitive
diagnosis of an ovarian mass is a common problem in gynecologic patients with adnexal mass. The routine standard evaluation
for adnexal masses includes patient's history, physical examination, ultrasound and histopathological examination. These parameters
individually or in combination have little predictive value. The accuracy of diagnostic tools are of immense value and great
concern to practicsing Gynecologists and Oncologists. The clinical application of serum concentration of CA 125, AFP and hCG
is of great help not only as diagnostic aid but also in monitoring efficacy of any treatment modality like chemotherapy, radiotherapy
or surgical resection. Additionally, evaluation of tumor marker concentration helps in predicting early biochemical recurrence
and in prognostication in different types of ovarian malignancies. The ability to differentiate a malignant mass from a benign
pelvic mass pretherapeutically could be enhanced optimally by additional use of tumor markers such as cancer antigen CA-125,
alphafetoprotein and human chorionic gonadotrophin in pre-and postmenopausal women. 相似文献
8.
Free radical generation and lipid peroxidation among the dry cell industry workers exposed to carbon
K. K. Reddy T. P. K. Reddy B. V. Somasekharaiah K. Soorya Kumari 《Indian journal of clinical biochemistry : IJCB》1998,13(1):27-32
A total of 75 healthy male dry cell industry workers exposed to carbon for 5, 8 and 10 years were compared with 48 controls
matched for age and economic status with respect to free radical generation, lipid peroxidation and antioxidant levels. Lymphocytes
were seperated from the whole blood and used for estimation of free radicals and antioxidants. Plasma lipid peroxidation products
were estimated in the form of thiobarbituric acid reactive substances. Significant increase in free radical generation and
lipid peroxidation products were observed in carbon exposed population than controls, and the increase was found to be significant
with increase in the period of exposure. The levels of antioxidants i.e., superoxide dismutase (SOD) and catalase were found
to decrease with the increase in exposure to carbon in industrial workers. These results suggest that exposure to carbon augments
free radical generation, lipid peroxidation and promotes decline in antioxidant enzymes. 相似文献
9.
Vipin K. Agrawal Poonam Khanna Vijay K. Agrawal Larry W. Hughes 《Decision Sciences Journal of Innovative Education》2020,18(3):481-516
Ongoing curriculum change in higher education is essential to enhance student learning and better prepare them for the job‐market. However, research shows that faculty are reluctant to implement such changes because students generally react adversely thereby negating any potential benefits, and moreover, sanction faculty through lower evaluations and future enrollment. Yet, understanding of the effect of curriculum changes on students’ course and instructor perceptions is limited. In this article we attempt to fill this gap. Drawing on two empirical findings—students’ motivation to attend college becoming increasingly extrinsic since the 1960s and their inability to recognize, ex ante, the value‐added by a curriculum change—and the norm life‐cycle theory, we argue that any effort‐increasing or grade‐threatening change is viewed as non‐normative and will lead to an adverse student reaction. However, this adverse reaction will dissipate over time once a critical mass of students is convinced of the merits of the new curriculum. We find support for our hypotheses by analyzing change in student perceptions following curriculum changes at a U.S. University. In addition, we also find that once the adverse reaction dissipates, students’ perceptions of the new curriculum become more positive than the old curriculum, only to be reversed once the revised curriculum is accepted as the new norm. 相似文献
10.
Hemant Kumar Ashok Kumar Poonam Kumari N. B. Tulsani 《Indian journal of clinical biochemistry : IJCB》2000,15(2):124-127
We have developed a biostrip for determination of urea in serum. The test strip is based on enzymatic assay where urease has
been immobilized on the chromatographic paper along with chromogen, phenol red. The chromogen is easily soluble in water and
does not require other components for the color change. Serum urea reacts with urease and water to liberate ammonia and carbon
dioxide. The liberated ammonia changes the pH of the reaction medium, which is monitored by the chromogen phenol red. A single
step working reagent strip has been developed and the reaction is completed within 50 seconds at room temperature. With this
test strip urea concentration is measured in serum as low as 0.15 g/L. The speed and convenience of determining urea in serum
by this strip instantly makes it well suited for individuals, physicians and emergency centres. 相似文献