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Computational models that ensure accurate and fast responses to the variations in operating conditions,such as the cell temperature and relative humidity(RH),are essential monitoring tools for the real-time control of proton exchange membrane(PEM) fuel cells.To this end,fast cell-area-averaged numerical simulations are developed and verified against the present experiments under various RH levels.The present simulations and measurements are found to agree well based on the cell voltage(polarizat...  相似文献   
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Carboxylate-modified gold nanoparticles (GNPs) were synthesized in a simple one-step process based on the reduction of tetrachloroauric acid by aspartic acid in water. GNPs were identified by UV–Vis spectroscopy, dynamic light scattering (DLS) and transmission electron microscopy. Conjugation of protein molecules with functionalized nanoparticles was performed through electrostatic interaction. The GNP–protein conjugates were characterized by gel electrophoresis. The interaction between functionalized GNPs and protein molecules lead to conformational transition of protein structure after conjugation of protein with GNPs. This process was investigated by fluorescence spectroscopy and circular dichroism spectroscopy.  相似文献   
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Median noise barriers, like parallel noise barriers, can be employed to reduce the impact of traffic on roadside communities via the direct propagation path. The performance of different shapes of median barriers was compared using reactive and passive surfaces and a 2D boundary element method (BEM). In the case of reactive surfaces, quadratic residue diffusers (QRDs) and primitive root diffusers (PRDs) were used on the top and stem surfaces of median barriers. To introduce passive barriers, two different absorbent materials including fibrous material and a grass surface with flow resistivity of 20 000 and 2500 kg/(s·m2), respectively, were similarly applied. The effect of thin absorptive barriers was similar at lower frequencies and better at mid and high frequencies to that of their equivalent rigid barriers. More improvement was achieved by covering the top surface of thick barriers with grass rather than with fibrous material. The performance of QRD and PRD barriers where the diffuser was located on the top surface was more frequency dependent than that of barriers coated with fibrous material. A comparison of the average A-weighted insertion loss in the thick barriers showed that the greatest improvement (2.59 dB (A)) was achieved using a barrier of 30-cm thickness covered with grass.  相似文献   
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The purpose of this study is to propose a framework to evaluate the entrepreneurship intensity (EI) of Iranian state universities. In order to determine EI, a hybrid multi-method framework consisting of Delphi, Analytic Network Process (ANP), and VIKOR is proposed. The Delphi method is used to localize and reduce the number of criteria extracted from a deep literature review, according to the social and economic conditions of Iranian state universities by using an expert panel, including sixty-eight country-wide academicians and practitioners. After that, a group approach to ANP was utilized as an evaluation method to derive the weights of each criterion. Next, the evaluation data were gathered through a questionnaire, and, finally, the compromise ranking of universities was calculated using the VIKOR method. Moreover, this study applies weight-variance analysis (WVA) to suggest improvement actions. The paper proposes an evaluation framework for determining the performance of entrepreneurship development initiatives in universities. By using this framework, twenty-two Iranian state universities are evaluated in terms of their EI, and the results are discussed.  相似文献   
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Viral infections remain a major threat to public health. The speed with which viruses are evolving drug-resistant mutations necessitates the further development of antiviral therapies with a large emphasis on drug discovery. To facilitate these efforts, there is a need for robust, high-throughput assays that allow the screening of large libraries of compounds, while enabling access to detailed kinetic data on their antiviral activity. We report here the development of a droplet-based microfluidic platform to probe viral fusion, an early critical step in infection by membrane-enveloped viruses such as HIV, Hepatitis C, and influenza. Using influenza A, we demonstrate the measurement of the kinetics of fusion of virions with target liposomes with sub-second temporal resolution. In analogy with acidification of the endosome that triggers fusion in a cellular context, we acidify the content of aqueous droplets containing virions and liposomes in situ by introducing acid from the dispersed phase and visualize the kinetics of fusion by using fluorescent probes.  相似文献   
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