One step antibody-mediated isolation and patterning of multiple cell types in microfluidic devices     

Danny Bavli  Elishai Ezra  Daniel Kitsberg  Margarita Vosk-Artzi  Shashi K. Murthy  Yaakov Nahmias 《Biomicrofluidics》2016,10(2)

Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.  相似文献   

Interaction of LDL and platelets in ischaemic and ischaemic risk subjects     

Yashika Gupta  V. Mallika  D. K. Srivastava 《Indian journal of clinical biochemistry : IJCB》2005,20(1):87-94

Platelets play a vital role in the progression of atherosclerosis and thrombosis, a major cause of death worldwide. Platelets are activated by many triggers like elevated LDL in blood resulting in aggregation and formation of plaque. The purpose of this study was to investigate the effect of LDL and signal transduction inhibitor on the activation of platelets in Ischaemic risk subjects. Platelets from IHD and hyperlipidemic subjects were hypersensitive to ADP, as higher levels of platelet aggregation were observed in these groups. LDL from IHD hyperlipidemic subjects was more effective in activating platelets from any other group. Ox-LDL was more effective in activating platelets than native-LDL as monitored by level of platelet aggregation induced by PAF and thrombin. Calcium channel blocker, nifedipine and verapamil inhibited platelet aggregation at micromolar level. Protein kinase inhibitor, staurosporine was effective in inhibiting ADP induced aggregation at nanomolar level.  相似文献   

Gödel’s proof     

S M Srivastava 《Resonance》2007,12(2):59-70

In 1931, the great Austrian mathematician Kurt Gödel proved “all consistent axiomatic formulations of number theory include undecidable propositions”. This discovery of Gödel and its proof had enormous repercussions in mathematics and computer science. The proof hinged upon the writing of a self-referential mathematical statement, in the same way as the liar’s paradox — I am lying — is a self-referential statement. In this three-part article, we describe Gödel’s discovery and his famous proof.  相似文献   

Book reviews     

Finbarré Fitz Patrick  Nicholas Hawkes  James Cummins  Alison M. Pask  Carol Myers Scotton  R. N. Srivastava 《International Review of Education/Internationale Zeitschrift für Erziehungswissenschaft/Revue internationale l'éducation》1978,24(3):423-434

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Examining the discourse on waste in school education from the standpoint of marginalized communities in Mumbai     

Srivastava  Himanshu  Gupta  Ankush  Raveendran  Aswathy 《Cultural Studies of Science Education》2022,17(2):301-330

Cultural Studies of Science Education - The M(East) ward of Mumbai is home to one of Asia’s largest open landfill sites. Waste, therefore, is a source of livelihood for millions of people...  相似文献   

Some innovative initiatives in literacy,post-literacy and continuing education in Asia     

H. S. Srivastava 《International Review of Education/Internationale Zeitschrift für Erziehungswissenschaft/Revue internationale l'éducation》1985,30(3):369-375

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Genetics of Obesity     

Apurva Srivastava  Neena Srivastava  Balraj Mittal 《Indian journal of clinical biochemistry : IJCB》2016,31(4):361-371

Numerous classical genetic studies have proved that genes are contributory factors for obesity. Genes are directly responsible for obesity associated disorders such as Bardet–Biedl and Prader–Willi syndromes. However, both genes as well as environment are associated with obesity in the general population. Genetic epidemiological approaches, particularly genome-wide association studies, have unraveled many genes which play important roles in human obesity. Elucidation of their biological functions can be very useful for understanding pathobiology of obesity. In the near future, further exploration of obesity genetics may help to develop useful diagnostic and predictive tests for obesity treatment.  相似文献   

Hyperhomocysteinemia,MMPs and Cochlear Function: A Short Review     

Seema Bhargava  Meghaa Shanta Bhargava  Eishaan Kamta Bhargava  LM Srivastava 《Indian journal of clinical biochemistry : IJCB》2016,31(2):148-151

Hyperhomocysteinemia (HHCY) has been demonstrated to affect cochlear microvasculature as well as cochlear epithelial cells directly, with a resultant alteration of the expression of matrix metalloproteinases (MMPs) and their inhibitors, tissue inhibitors of metalloproteinases (TIMPs). Hence, ascertaining the optimum concentration of MMPs and TIMPs in the cochlea could help to inhibit hearing loss due to HHCY by the administration of appropriate MMP inhibitors, Since infections/inflammations as well as ototoxic antibiotics have a similar mechanism of otic pathology, the cochlear damage they cause could also be similarly prevented.  相似文献   

Individualism-collectivism and business context as predictors of behaviors in cross-national work settings: Incidence and outcomes     

Peter B. Smith  Cláudio V. TorresAlena Chudzikova  Serdar DegirmenciogluFrancisco Donoso-Maluf  Nancy Chen Yi FengCharles Harb  Brad JacksonSigmar Malvezzi  Andrew MogajiJuan Carlos Pastor  Lorena Perez-FlorianoB.N. Srivastava  Günter StahlStephanie Thomason  Vladimir Yanchuk 《Int J Intercult Relat》2011,35(4):440-451

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Effects of roller burnishing process parameters on surface roughness of A356/5%SIC composite using response surface methodology     

Shashi Prakash Dwivedi Satpal Sharma Raghvendra Kumar Mishra 《上海大学学报(英文版)》2014,(4):303-317

In this study, a simple roller burnishing tool was made to operate burnishing processes on A356/5%SIC metal matrix composite fabricated by electromagnetic stir casting under different parameters. The effects of burnishing speed, burnishing force and number of burnishing passes on the surface roughness and tribological properties were measured. Scanning electron microscopy （SEM） graphs of the machined surface with PCD （insert-10） tool and roller burnished surface with tungsten carbide （WC） roller were taken into consider- ation to observe the surface finish of metal matrix composites. The mechanical properties （tensile strength, hardness, duc- tility） of A356/5%SIC metal matrix composites were studied for both unburnished samples and burnished samples. The results revealed that the roller burnished samples of A356/ 5%SIC led to the improvement in tensile strength, hardness and ductility. In order to find out the effects of roller bur- nishing process parameters on the surface roughness of A356/ 5%SIC metal matrix composite, response surface methodol- ogy （RSM） （Box-Behnken design） was used and a prediction model was developed relevant to average surface roughness using experimental data. In the range of process parameters, the result shows that roller burnishing speed increases, and surface roughness decreases, but on the other hand roller burnishing force and number of passes increase, and surface roughness increases. Optimum values of burnishing speed （1.5 m/s）, burnishing force （50 N） and number of passes （2） during roller burnishing of A356/5%SIC metal matrix com- posite to minimize the surface roughness （predicted 1.232 μm） have been found out. There was only 5.03% error in the experimental and modeled results of surface roughness.  相似文献