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司马迁有言,“《诗》三百篇,大抵圣贤发愤之所为作也”（《史记·太史公自序》）。倘若如此,《诗》必定立意深远、极高明而道中庸。可是。后来的朱熹却说：“吾闻之,凡《诗》之所谓‘风’者,多出于里巷歌谣之作,所谓男女相与咏歌,各言其情者也。”（朱熹,《诗集传·序》）倘若如此,至少《诗》中的《风》就谈不上立意深远、极高明而道中庸,不仅如此,历代经师的诗说反倒阻碍了我们欣赏清新淳朴的民歌。朱熹的《诗集传》作为宋儒解《诗》的集大成之作,统治后世七八百年,为什么这位宋代大儒会主张《国风》出于民间说？为什么《诗经》乃至五经,在宋代的地位与解释路向,会发生翻天覆地的变化？年前,见《诗集传》出了新的点校本,这些问题霎时又变得新鲜起来。 相似文献
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The long-arm and short-arm genes of fibroin light chain (L-chain) of silkworm, Bombyx Mori L., and the gene of human acidic fibroblast growth factor were cloned respectively and subsequently inserted into a transfer vector pVL1392 used as a tool to target the L-chain region of the silkworm genome. Genomic DNA from their offsprings was extracted and the expected targeting was detected using polymerase chain reaction and DNA sequencing, as well as protein analysis. The results showed that positive events occurred and that the FGF gene was integrated into the L-chain locus through homologous recombination. 相似文献
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数学变式教学是通过变更数学概念的非本质特征来暴露问题本质特征的敦学方法．即在数学教学中用不同形式的直观材料或事例说明概念、定理、命题的本质属性,对数学的定理和命题的非本质特征进行不同角度、不同层次、不同情形、不同背景的变化,以突出它们的本质特征,揭示不同知识点的内在联系的一种教学方法．包括知识形成过程中的问题设计, 相似文献
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INTRODUCTION Transgenic technology developed over the 20years, has become a common tool widely applied tolivestock species because it is technically quitesimple, requiring only the injection of ‘naked’DNA into the nucleus of a fertilized eggs (Hammeret al., 1985; Suraokar and Bradley, 2000). Howeverthis technique has limitations in that the injectedDNA integrates randomly into the genome and thuscould not be expressed in the desired tissue or at theappropriate level. More impor… 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＦｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ (ＦＧＦ)ｉｓａｎａｎｇｉｏ ｇｅｎｉｃａｎｄｍｉｔｏｇｅｎｉｃｐｏｌｙｐｅｐｔｉｄｅｔｈａｔｃａｎｐｒｏｍｏｔｅｃｅｌｌｐｒｏｌｉｆｅｒａｔｉｏｎａｎｄｄｉｆｆｅｒｅｎｔｉａｔｉｏｎｉｎａｗｉｄｅｖａ ｒｉｅｔｙｏｆｃｅｌｌｔｙｐｅｓ (Ｇｏｌｄｆａｒｂ ,1 990 ;Ｂａｓｉｌｉｃｏｅｔａｌ.,1 992 ) .ＴｈｅＦＧＦｆａｍｉｌｙｈａｓｍａｎｙｍｅｍｂｅｒｓｗｉｔｈｓｉｍｉｌａｒａｍｉｎｏａｃｉｄｓｅｑｕｅｎｃｅａｎｄｂｉｏｌｏｇｉｃａｌａ… 相似文献
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吴小锋 《晋城职业技术学院学报》2013,6(2):73-75
构式作为形式和意义的结合体,是对世界进行概念化的一种表达方式。英语中body-part ascension构式是一种具有独特句法和语义的表达方式,反映了人类基本生活经验的场景。 相似文献
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随着物联网技术的迅速普及和发展,物联网安全问题也越来越令人担忧.针对这一问题,首先逐一分析了物联网各层所面临的安全威胁,然后讨论了解决这些安全问题的关键技术,设计出一种基于分层结构的物联网安全模型,并将这种模型应用到智慧校园中.实践结果表明,通过逐层逐级防护,可以有效提高物联网的安全性. 相似文献
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Human acidic and basic fibroblast growth factors (aFGF and bFGF) are classic and well characterized members of the heparin-binding
growth factor family. Heparin is generally thought to play an extremely important role in regulating aFGF and bFGF bioactivities
through its strong binding with them. In order to unravel the mechanism of the interactions between heparin and FGFs, and
evaluate the importance of heparin sulfate groups' binding with FGFs, surface plasmon resonance analyses were performed using
IAsys Cuvettes System. Heparin and its regioselectively desulfated derivatives were immobilized on the cuvettes. aFGF and
bFGF solutions with different concentrations were pipetted into the cuvettes and the progress of the interaction was monitored
in real-time by Windows-based software, yielding kinetic and equilibrium constants for these interactions. In addition, in
order to reduce the delicate difference among the cuvettes, inhibition analyses of mixtures of FGFs and immobilized native
heparin by modified heparins were also done. The data from these two methods were similar, indicating that all sulfate groups
at 2-O, 6-O and N- in heparin were required for the binding to aFGF; and that their contribution to the binding was in the
order 2-O, N- and 6-O-sulfate group. In contrast, definite contribution of the 6-O-sulfate group to the binding with bFGF
was most apparent, while the other two sulfate groups appeared to be necessary in the order 2-O and N-sulfate group. These
methods established here can be used for analysing the effect of sulfate groups in heparin on the binding with other human
FGF members or other heparin-binding proteins.
The project supported by the Scientific Research Foundation for Returned Overseas Chinese Scholars, State Education Ministry
of China and Zhejiang Provincial Natural Science Foundation of China (301306) 相似文献
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