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Jagdish S. Gundara 《比较教育学》2000,36(2):223-234
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Alex Livy Sayhean Lye Chahil K. Jagdish Nurul Hanis Velapasamy Sharmila Lian Wee Ler Bagali Pramod 《Indian journal of clinical biochemistry : IJCB》2012,27(1):28-33
Buccal cell usage has been shown by many to be a cost effective and safe method to isolate DNA for various biological experiments
especially large epidemiological studies (Garcia-Closas et al. Cancer Epidemiol Biomarkers Prev 10:687–696, 2001). Non-invasive DNA collection methods are preferred over phlebotomy in order to increase study participation and compliance
in research centers and for sick patients in hospital settings. There have been conflicting reports about the methodology
and results obtained from using buccal DNA. It is not very clear if phlebotomy can be confidently replaced by buccal cell
DNA. It is often left for the user to take an intelligent decision. To address this issue, we compared the performance of
buccal and blood DNA from same subjects in a genotyping experiment and this paper reports the results. Cotton swab derived
buccal cells were scraped from the inner side of cheeks from 16 subjects, and blood was also drawn from the same 16 subjects
participating in a genotypic association study of a lipid disease. The DNA quality was assessed by resolving on agarose gels,
checking purity (A260/A280) and finally by microarray hybridization. This study showed that DNA degradation affects the total
yield and performance of the buccal DNA when compared to the blood DNA in microarray based genotyping. Genotyping results
can be seriously compromised if care is not taken to check the quality and yields of such specimens. 相似文献
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Kilauea volcano in the Hawaiian Islands, Pacific Ocean, is the world’s most active volcano. Observations of active lava flows
of Kilauea have a great relevance to studies of older, extinct volcanic systems, such as those found in India. 相似文献
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Kavita K. Shalia Manoj R. Mashru Jagdish B. Vasvani Rajashree A. Mokal Shilpa M. Mithbawkar Priti K. Thakur 《Indian journal of clinical biochemistry : IJCB》2009,24(4):388-397
Hypertension causes complications such as coronary atherosclerosis and thrombosis wherein inflammatory factors play significant
role. In the present study inflammatory molecules such as cell adhesion molecules (CAMs); endothelial (E)-selectin, platelet
(P)-selectin, intercellular CAM-1 (ICAM-1), vascular CAM-1 (VCAM-1) and platelet endothelial CAM-1 (PECAM-1) were analysed
in subjects newly diagnosed with hypertension with no secondary cause against normotensive healthy individuals. In each group
57 subjects were recruited and soluble (s) levels of CAMs were analysed by ELISA. As compared to controls median of sE-selectin
(49.2%, P=0.001), sP-selectin (54.3%, P=0.001), and sICAM-1 (18.9%, P=0.012) were significantly elevated in hypertensive subjects.
Significant negative correlation was observed of sP-selectin (spearman rank correlation coefficient (rs) =−0.345, p=0.027)
and sPECAM-1 (rs =−0.446, p=0.003) with age in hypertension group. Hypertension may increase expression of certain CAMs while
younger hypertensives in addition are also at increased risk of atherothrombosis. 相似文献
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Jagdish Kaur Chahil Khamsigan Munretnam Nurulhafizah Samsudin Say Hean Lye Nikman Adli Nor Hashim Nurul Hanis Ramzi Sharmila Velapasamy Ler Lian Wee Livy Alex 《Indian journal of clinical biochemistry : IJCB》2015,30(2):134-139
Genome-wide association studies have discovered multiple single nucleotide polymorphisms (SNPs) associated with the risk of common diseases. The objective of this study was to demonstrate the replication of previously published SNPs that showed statistical significance for breast cancer in the Malaysian population. In this case–control study, 80 subjects for each group were recruited from various hospitals in Malaysia. A total of 768 SNPs were genotyped and analyzed to distinguish risk and protective alleles. A total of three SNPs were found to be associated with increased risk of breast cancer while six SNPs showed protective effect. All nine were statistically significant SNPs (p ≤ 0.01), five SNPs from previous studies were successfully replicated in our study. Significant modifiable (diet) and non-modifiable (family history of breast cancer in first degree relative) risk factors were also observed. We identified nine SNPs from this study to be either conferring susceptibility or protection to breast cancer which may serve as potential markers in risk prediction.