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Highly motile Phytophthora sojae (P. sojae) zoospores of an oomycete plant pathogen and antioomycete candidate chemicals were encapsulated into microdroplets. Random fast self-motion of P. sojae zoospores was overcome by choosing an appropriate flow rate for a zoospore suspension. To influence stochastic loading of zoospores into a microfluidic channel, a zoospore suspension was directly preloaded into a microtubing with a largely reduced inner diameter. A relatively high single zoospore encapsulation rate of 60.5% was achieved on a most trivial T-junction droplet generator platform, without involving any specially designed channel geometry. We speculated that spatial reduction in the diameter direction of microtubing added a degree of zoospore ordering in the longitudinal direction of microtubing and thus influenced positively to change the inherent limitation of stochastic encapsulation of zoospores. Comparative phenotypic study of a plant oomycete pathogen at a single zoospore level had not been achieved earlier. Phenotypic changes of zoospores responding to various chemical concentration conditions were measured in multiple droplets in parallel, providing a reliable data set and thus an improved statistic at a low chemical consumption. Since each droplet compartment contained a single zoospore, we were able to track the germinating history of individual zoospores without being interfered by other germinating zoospores, achieving a high spatial resolution. By adapting some existing droplet immobilization and concentration gradient generation techniques, the droplet approach could potentially lead to a medium-to-high throughput, reliable screening assay for chemicals against many other highly motile zoospores of pathogens. 相似文献
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Surabhi Verma Som Sekhar Bhattacharyya Saurav Kumar 《Information processing & management》2018,54(5):791-806
Research on the adoption of systems for big data analytics has drawn enormous attention in Information Systems research. This study extends big data analytics adoption research by examining the effects of system characteristics on the attitude of managers towards the usage of big data analytics systems. A research model has been proposed in this study based on an extensive review of literature pertaining to the Technology Acceptance Model, with further validation by a survey of 150 big data analytics users. Results of this survey confirm that characteristics of the big data analytics system have significant direct and indirect effects on belief in the benefits of big data analytics systems and perceived usefulness, attitude and adoption. Moreover, there are mediation effects that exist among the system characteristics, benefits of big data analytics systems, perceived usefulness and the attitude towards using big data analytics system. This study expands the existing body of knowledge on the adoption of big data analytics systems, and benefits big data analytics providers and vendors while helping in the formulation of their business models. 相似文献
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A simple theory is presented for decoupling a pair of lossless coupled nonuniform transmission lines (CNUTL), with a common return and supporting TEM waves, into two lossless nonuniform transmission lines (NUTL). This theory is independent of the port terminations, symmetry conditions, etc. The method directly relates the line parameters of the lossless CNUTL's to those of the decoupled lines and vice versa; further, the matrix parameters of the CNUTL's as a four-port are explicitly expressed in terms of those of the decoupled lines as two-ports. This theory is then utilized to study the applications of CNUTL's as directional couplers.It is shown that for CNUTL's to behave as a codirectional coupler, each of the decoupled lines should be a proportional line, while for contradirectional coupler action, the two decoupled lines have to be duals of each other. The coupling response of the codirectional coupler is found to be periodic, while the phase shift between coupled and transmitted signals varies linearly with frequency. The coupling response of various contradirectional couplers with smooth transition at one of the ends, for which the decoupled lines are “basic NUTL's with hyperbolic solutions”, are studied in detail. It is shown that all these couplers have a high-pass response and that the CNUTL's with “hyperbolic cosine squared lines” as decoupled lines have the best response of all the CNUTL's considered. 相似文献
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J. C. Bhattacharyya 《Resonance》1998,3(5):24-29
This article surveys important developments in the growth of spectroscopy and its application to the study of stellar spectra. Among others, the contributions of Wollaston, Fraunhofer, Kirchhoff, Bunsen and Doppler are highlighted. 相似文献
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Asit Bhattacharyya 《Environmental Education Research》2016,22(4):590-610
Efforts to promote corporate societal and environmental accountability (SEA) should be informed by an understanding of stakeholders’ attitudes toward enhanced accountability standards. However, little is known regarding current attitudes on this subject or the determinants of these attitudes. To address this issue, this study examines the relationship between personal values and support for social and environmental accountability for a sample of Indian MBA students. Selected contemporary social and environmental issues were used to assess the students’ support for social and environmental accountability. Principal component analysis was used to explore the underlying dimensions or factors that shape social and environmental beliefs and attitudes. Multiple regression models were run for each of the SEA dimensions to test the hypotheses and the relationship between the values and support for SEA. Exploratory factor analysis of the items revealed two distinct factors: (1) endorsement of the general proposition that corporations and executives should be held accountable for the social and environmental impacts of their actions and (2) agreement that the government should adopt and enforce formal SEA standards. The results indicate that the security and universalism value type is positively associated with general support for SEA but not with support for government enforcement of accountability standards. The study found that the benevolence value type is negatively associated with general support for SEA. Gender also has a significant impact on support for government enforcement of SEA standards. 相似文献
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Sadhan Basu was an inspiring teacher and scientist and among the best that our university system has produced. He carried out important and thoughtful research work in the fields of polymers, spectroscopy and quantum chemistry that bear the signature of a highly creative mind. And all these he did with so little resources at his disposal. No wonder that his memory still inspires awe and respect among many of his immensely successful students and colleagues. 相似文献