排序方式： 共有33条查询结果，搜索用时 93 毫秒
1.
Reduction of water evaporation in polymerase chain reaction microfluidic devices based on oscillating-flow 总被引：1，自引：0，他引：1
Alessandro Polini Elisa Mele Anna Giovanna Sciancalepore Salvatore Girardo Adriana Biasco Andrea Camposeo Roberto Cingolani David A. Weitz Dario Pisignano 《Biomicrofluidics》2010,4(3)
Producing polymeric or hybrid microfluidic devices operating at high temperatures with reduced or no water evaporation is a challenge for many on-chip applications including polymerase chain reaction (PCR). We study sample evaporation in polymeric and hybrid devices, realized by glass microchannels for avoiding water diffusion toward the elastomer used for chip fabrication. The method dramatically reduces water evaporation in PCR devices that are found to exhibit optimal stability and effective operation under oscillating-flow. This approach maintains the flexibility, ease of fabrication, and low cost of disposable chips, and can be extended to other high-temperature microfluidic biochemical reactors. 相似文献
2.
Approaches to environmental education which are engaging with place and critical pedagogy have not yet broadly engaged with the African world and insights from Africana Studies and Geography. An African-centred approach facilitates people’s reconnection to places and ecosystems in ways that do not reduce places to objects of conquest and things to be exploited for profitability and individual gain. Such an approach offers effective critiques of settler coloniser perspectives on the environment and deeper understandings of the relationship between worldview and ecologically sensitised education. Through examples from Africana Studies and Geography, this article provides an introduction to how an African-centred approach can contribute to the development of a Land Education perspective and improve college-level environmental education. 相似文献
3.
Promoting Vocational Development: Methods of Intervention
and Techniques Used in the Italian Context
Laura?NotaEmail author Salvatore?Soresi Scott?H.?Solberg Lea?Ferrari 《International Journal for Educational and Vocational Guidance》2005,5(3):271-279
This article describes a Master Educator Series designed to help educators in Italy design and implement comprehensive vocational guidance curriculum. In this “Master Educator” training, a variety of vocational development models and theories are presented. The goal is to provide a broad range of skills in assessment and intervention strategies to prepare educators for developing the curriculum that best fits their unique context. Five curriculum examples are described. Each of these interventions is built around a vocational development topic, including: providing individualized feedback, improving social relationships and connections, using written exercises, and providing mastery and vicarious experiences. 相似文献
4.
5.
6.
7.
Gea O. F. Parikesit Jeffrey S. Guasto Salvatore Girardo Elisa Mele Ripalta Stabile Dario Pisignano Ralph Lindken Jerry Westerweel 《Biomicrofluidics》2009,3(4)
Nanoparticle image velocimetry (nano-PIV), based on total internal reflection fluorescent microscopy, is very useful to investigate fluid flows within ∼100 nm from a surface; but so far it has only been applied to flow over smooth surfaces. Here we show that it can also be applied to flow over a topologically structured surface, provided that the surface structures can be carefully configured not to disrupt the evanescent-wave illumination. We apply nano-PIV to quantify the flow velocity distribution over a polydimethylsiloxane surface, with a periodic gratinglike structure (with 215 nm height and 2 μm period) fabricated using our customized multilevel lithography method. The measured tracer displacement data are in good agreement with the computed theoretical values. These results demonstrate new possibilities to study the interactions between fluid flow and topologically structured surfaces. 相似文献
8.
9.
10.
Kwon Kyong-Ah Ford Timothy G. Salvatore Alicia L. Randall Ken Jeon Lieny Malek-Lasater Adrien Ellis Natalie Kile Mia S. Horm Diane M. Kim Sun Geun Han Minkyung 《Early Childhood Education Journal》2022,50(1):169-171
Early Childhood Education Journal - The original version of this article unfortunately contains some typo in Table 1. 相似文献