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Gian Paolo Caviglia Luca Cabianca Sharmila Fagoonee Fabrizio M. Gili 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2016,26(1):114-120
Introduction
Screening programs for colorectal cancer (CRC) are mainly based on a first-line fecal immunochemical test for hemoglobin (FIT). Fecal M2-type pyruvate kinase (M2-PK) has been evaluated in clinical settings showing promising results for early CRC detection. However, the impact of fecal M2-PK assessment on the performance of first-round CRC screening programs is not known. We investigated whether fecal M2-PK alone or in combination with FIT may improve CRC screening efficacy in the general population.Materials and methods
A total of 1027 asymptomatic subjects (median age 66 [59-74] years; females 504 [49.1%]), identified through the general practitioners’ rosters, were invited for the collection of 2 fecal samples for FIT and M2-PK evaluation. Participants with at least positive one fecal test were referred for colonoscopy. Quality indicators for screening performance were calculated and analyzed using Fisher’s exact test.Results
Overall, 572 subjects underwent both FIT and M2-PK assessment (participation rate 55.7%): 93 participants showed positive results for at least one test (positivity rate 16.3%). Only 10 patients were positive for both tests. Attendance rate to colonoscopy was 86.0% and a total of 65 adenomas and 7 cancers were detected. Combined use of FIT and fecal M2-PK permitted the identification of 18 more neoplasm (25%) without improving colonoscopy workload, as deduced by the comparable number needed to scope (P = 0.402).Conclusion
The addition of M2-PK testing to FIT offers the potential to detect additional neoplasms that either do not bleed or only bleed intermittently without reducing participation rate and without increasing endoscopy workload.Key words: biomarker, colorectal cancer, occult blood, M2-type pyruvate kinase, screening 相似文献2.
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Sharmila?JayasingamEmail author Mahfooz A?Ansari T?Ramayah Muhamad?Jantan 《知识管理研究与实践》2013,11(3):255-264
Knowledge management (KM) has often been claimed to be an essential ingredient in building competitive advantage. Yet, KM adoption is relatively slow, especially in the Malaysian context. Most organizations are unsure if the promised performance improvement is just a passing fad. This study intends to provide empirical verifications to support the link between KM practices and performance outcomes for organizations. Responses from 180 knowledge-based organizations were analysed. The analysis indicated that knowledge acquisition and knowledge utilization positively influenced strategic and operational improvement in organizations, whereas the positive effect of knowledge dissemination was only evident in the case of strategic improvement. Organization size had some interesting moderating impact on the tested relationships. 相似文献
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Merin Iype Subramanya Upadhya Sharmila Upadhya Gopalakrishna Bhat 《Indian journal of clinical biochemistry : IJCB》2008,23(4):401-403
Experimental model of chronic renal failure in rats have been described by many authors and has also been widely used in various
studies. Many of these methods used highly sophisticated instruments which was difficult in our settings. The resection model
was ideal in our experimental set-up, but this model had the risk of excessive bleeding and hypovolemia. In our study we used
a combination of partial resection and ligation of the renal artery to create two models of stable uremia A and Moderate uremia
B. Severe uremia. Both these models were compared with a sham operated group which served as controls. Following surgical
procedure, the development of uremia was monitored by serial estimation of blood urea and serum creatinine levels that were
measured at regular intervals (bi-weekly). From two weeks onwards the animals in the experimental group showed a significant
elevation in the serum urea levels and a consistent elevation in the serum creatinine levels upto eight weeks when compared
to the animals in the sham operated group. We established a modified method of producing renal failure which can be maintained
for a period of six weeks. This model is simple, reproducible and less complicated that can be used for several studies relating
to renal failure in the field of research. 相似文献
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AbstractShoreline Community College is near Seattle in Washington state, a growing community that has evolving needs. The library at the college planned and implemented events for National Poetry Month to get the students, faculty, and community engaged through activities such as White Board Poetry, Spoken Word Poetry, a Film Showing, and a Haiku Contest. Promotional planning and follow through focused on campus resources, student engagement, and social media. 相似文献
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Alex Livy Sayhean Lye Chahil K. Jagdish Nurul Hanis Velapasamy Sharmila Lian Wee Ler Bagali Pramod 《Indian journal of clinical biochemistry : IJCB》2012,27(1):28-33
Buccal cell usage has been shown by many to be a cost effective and safe method to isolate DNA for various biological experiments
especially large epidemiological studies (Garcia-Closas et al. Cancer Epidemiol Biomarkers Prev 10:687–696, 2001). Non-invasive DNA collection methods are preferred over phlebotomy in order to increase study participation and compliance
in research centers and for sick patients in hospital settings. There have been conflicting reports about the methodology
and results obtained from using buccal DNA. It is not very clear if phlebotomy can be confidently replaced by buccal cell
DNA. It is often left for the user to take an intelligent decision. To address this issue, we compared the performance of
buccal and blood DNA from same subjects in a genotyping experiment and this paper reports the results. Cotton swab derived
buccal cells were scraped from the inner side of cheeks from 16 subjects, and blood was also drawn from the same 16 subjects
participating in a genotypic association study of a lipid disease. The DNA quality was assessed by resolving on agarose gels,
checking purity (A260/A280) and finally by microarray hybridization. This study showed that DNA degradation affects the total
yield and performance of the buccal DNA when compared to the blood DNA in microarray based genotyping. Genotyping results
can be seriously compromised if care is not taken to check the quality and yields of such specimens. 相似文献
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