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采用纳米金和牛血清白蛋白(BSA)-二氧化钛(TiO2)固载抗体制得灵敏度较高的甲胎蛋白(AFP)免疫传感器,采用了循环伏安法对传感器的制备过程进行表征.实验结果表明,该传感器对AFP有很好的电流响应,其线性范围为0.01~80.0ng/mL,检出限为0.003ng/mL.该实验方法具有电极制备简单,操作简便,灵敏度高等特点,实现了对AFP的定量分析. 相似文献
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T. Malati G. Rajani Kumari B. Yadagiri 《Indian journal of clinical biochemistry : IJCB》2001,16(2):224-233
Ovarian cancer is the fifth leading cause of death in women. The incidence of this malignancy increases in women over the
age of 40. The overall five years survival is less than 30%, as most women present with advanced stage disease. Until recently,
detection of early stage ovarian cancer has been difficult since it is usually nonpalpable and asymptomatic. The definitive
diagnosis of an ovarian mass is a common problem in gynecologic patients with adnexal mass. The routine standard evaluation
for adnexal masses includes patient's history, physical examination, ultrasound and histopathological examination. These parameters
individually or in combination have little predictive value. The accuracy of diagnostic tools are of immense value and great
concern to practicsing Gynecologists and Oncologists. The clinical application of serum concentration of CA 125, AFP and hCG
is of great help not only as diagnostic aid but also in monitoring efficacy of any treatment modality like chemotherapy, radiotherapy
or surgical resection. Additionally, evaluation of tumor marker concentration helps in predicting early biochemical recurrence
and in prognostication in different types of ovarian malignancies. The ability to differentiate a malignant mass from a benign
pelvic mass pretherapeutically could be enhanced optimally by additional use of tumor markers such as cancer antigen CA-125,
alphafetoprotein and human chorionic gonadotrophin in pre-and postmenopausal women. 相似文献
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目的:探讨孕妇年龄因素对孕中期(16周)羊水甲胎蛋白(AFP)浓度的影响。方法:采用电化学发光法,测定2005.1~12月来我院检查的怀孕16周孕妇羊水AFP浓度,将测定值按孕妇年龄分组(01组、02组、G3组)进行比较分析。结果:羊水AFP浓度随孕妇年龄增大而升高(P〈0.05),且4例确诊胎儿神经管缺陷孕妇年龄均大于30岁。结论:孕中期(16周)孕妇羊水AFP浓度受年龄因素影响,高龄孕妇羊水AFP浓度明显高于适龄孕妇。提示:高龄孕妇应做羊水AFP筛查检测,有助于提高神经管缺陷患儿的诊出率,避免患儿出生。 相似文献
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