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Bin Huang Yun Yin Lei Lu Hai Ding Lin Wang Ting Yu Jia-jin Zhu Xiao-dong Zheng Yan-zhen Zhang 《Journal of Zhejiang University. Science. B》2010,11(10):812-818
A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit
monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive
ELISA of CPFX had a concentration at 50% inhibition (IC50) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high
with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed
almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed
here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been
applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%–84.60%, with coefficients
of variation of less than 12.2%. 相似文献
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Hai-qin Zhong Shun Zhang Hong Pan Ting Cai 《Journal of Zhejiang University. Science. B》2013,14(9):837-843
Objective
The efflux pump (EP) is one of the major mechanisms of antibiotic resistance in Klebsiella pneumoniae. However, there are few reports on the effect of the abuse of antibiotic use on the activity of EPs. To determine whether the use of low efficacy antibiotics has any effect on the activity of EPs and induces drug resistance in K. pneumoniae, we investigated the effect of ciprofloxacin on the activity of EPs in K. pneumoniae strains.Methods
Sixteen susceptible K. pneumoniae strains were isolated from patients and their minimum inhibitory concentrations (MICs) of ciprofloxacin were measured in the absence and presence of the pump inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The strains were then induced with a gradient of ciprofloxacin until the MICs of the strains showed no further increase, to obtain induced resistant strains. The EP activities of the strains before and after induction were compared using EP inhibition and ethidium bromide (EtBr) accumulation assays.Results
The MIC values of the strains were 16–256 times higher after induction than before induction. In the presence of CCCP, the MIC values of 50% of the induced strains were 2–4-fold lower than that in the absence of this inhibitor. The EtBr accumulation assay showed that the fluorescence of EtBr in the induced cells was lower than that in the cells before induction.Conclusions
EPs are widespread in susceptible and drug-resistant K. pneumoniae strains. Induction with ciprofloxacin may increase the activity of EPs in K. pneumoniae. The EtBr accumulation assay is more sensitive than the EP inhibition assay in evaluating the activity of EPs in K. pneumoniae. 相似文献3.
目的：在原有生产条件下通过调整薄膜衣包衣技术寻找出影响盐酸环丙沙星片脆碎度的因素进而确定最佳工艺参数提高产品质量、降低生产过程中不合格品的数量。方法：通过分析影咆脆碎度的原因在相同工艺条件下找出主要影响因素确定最佳方案。结果：采取有效措施后提高了盐酸环丙沙星片的脆碎度减少了尾料数量。结论：采取的有效措施方法简单易行能保证产品质量。 相似文献
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Development of an indirect competitive ELISA for simultaneous detection of enrofloxacin and ciprofloxacin 总被引：4，自引：0，他引：4
Zhang HT Jiang JQ Wang ZL Chang XY Liu XY Wang SH Zhao K Chen JS 《Journal of Zhejiang University. Science. B》2011,12(11):884-891
Modified 1-ethyl-3-(3-dimethylaminopropy) carbodiimide (EDC) method was employed to synthesize the artificial antigen of enrofloxacin
(ENR), and New Zealand rabbits were used to produce anti-ENR polyclonal antibody (pAb). Based on the checkerboard titration,
an indirect competitive enzyme-linked immunosorbent assay (ELISA) standard curve was established. This assay was sensitive
and had a linear range from 0.6 to 148.0 μg/kg (R
2=0.9567), with the half maximal inhibitory concentration (IC50) and limit of detection (LOD) values of 9.4 μg/kg and 0.2 μg/kg, respectively. Of all the competitive analogues, the produced
pAb exhibited a high cross-reactivity to ciprofloxacin (CIP) (87%), the main metabolite of ENR in tissues. After optimization,
the matrix effects can be ignored using a 10-fold dilution in beef and 20-fold dilution in pork. The overall recoveries and
coefficients of variation (CVs) were in the ranges of 86%–109% and 6.8%–13.1%, respectively. It can be concluded that the
established ELISA method is suitable for simultaneous detection of ENR and CIP in animal tissues. 相似文献
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