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Xiu-ping Lu Bing-guang Xiao Yong-ping Li Yi-jie Gui Yu Wang Long-jiang Fan 《Journal of Zhejiang University. Science. B》2013,14(7):570-577
Diversity arrays technology (DArT) is a microarray-based marker system that achieves high throughput by reducing the complexity of the genome. A DArT chip has recently been developed for tobacco. In this study, we genotyped 267 flue-cured cultivars/landraces, including 121 Chinese accessions over five decades from widespread geographic regions in China, 103 from the Americas, and 43 other foreign cultivars, using the newly developed chip. Three hundred and thirty polymorphic DArT makers were selected and used for a phylogenetic analysis, which suggested that the 267 accessions could be classified into two subgroups, which could each be further divided into 2–4 sections. Eight elite cultivars, which account for 83% of the area of Chinese tobacco production, were all found in one subgroup. Two high-quality cultivars, HHDJY and Cuibi1, were grouped together in one section, while six other high-yield cultivars were grouped into another section. The 330 DArT marker clones were sequenced and close to 95% of them are within non-repetitive regions. Finally, the implications of this study for Chinese flue-cured tobacco breeding and production programs were discussed. 相似文献
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用扫描电镜观察比较了共培养中农杆菌与烟草、花叶芋相互作用的差异.在烟草幼苗叶盘、茎的断面,观察到大量的细菌附着。它们通过菌体与细胞壁紧密接触、或通过纤维丝连接附着到烟草的伤口表面。类似的附着现象在花叶芋茎段表面也观察到.农杆菌能以与在烟草中相同的附着方式附着到花叶芋幼苗茎段伤口表面,特别是对维管束内及维管束周围的组织具有最大的亲和性,大量地附着到茎段维管束区域,但不能附着到叶盘断面的叶肉细胞上. 相似文献
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不同药剂处理对低温逆境下烟草种子发芽和幼苗生长的影响 总被引：3，自引：0，他引：3
研究了不同药剂处理对低温逆境下不同耐寒力烟草品种种子发芽和幼苗生长的影响。采用不同浓度的外源脯氨酸、水杨酸、氯化钙、甘油、二甲基亚砜、乙二醇、聚乙二醇、丙二醇、烯效唑、脱落酸对不同耐寒力烟草品种种子浸种处理后,测定了低温逆境下不同烟草品种发芽及幼苗生长和生理生化指标变化。结果表明,脯氨酸、水杨酸、氯化钙、二甲基亚砜和聚乙二醇5种药剂浸种能显著促进烟草种子发芽,提高了低温逆境下幼苗根长、苗高、幼苗干鲜重以及超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性,其中10 g/L脯氨酸、120 mg/L水杨酸、15 mg/L氯化钙、30 ml/L二甲基亚砜和100 ml/L聚乙二醇综合效果较好,可以作为提高烟草种子及幼苗抗寒性的处理方法。 相似文献
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从预防(施用提取物24小时后接种病毒)、治疗(施用提取物24小时前接种病毒)两个不同时期接种心叶烟,测试了15种植物提取物对烟草花叶病毒(Tobacco mosaic virus,TMV)的抑制作用。结果表明:所有植物提取物对TMV具有不同程度的预防和治疗效果。 相似文献
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通过20／1年的小区试验表明,在移栽期和中耕期施用植物有机诱导抗病剂“多肽保”,可以促进大田烟株生长整齐、发育良好,对烟草赤星病的防效达到71．23％-78．83％,单位面积内产量增加7．97％-15．97％,产值增加11．00％～20．77％,为防治烟草赤星病提供了一条新途径． 相似文献
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为了在烟叶生产上有效利用植物有机诱导抗病剂＂多肽保＂,降低使用成本实现效益最大化.2011年,以不防病为空白对照,设置＂多肽保＂不同施用量和施用方式的11个处理,与当前生产上采取的化学防治措施的防效相比较.结果表明：1）＂多肽保＂仅苗期施用量0.1～0.2 g/株,有利于烟株的生长发育和获得较好的经济效益,增产、增值幅度分别达到25.0%和35.5%;苗期施用量达到或超过0.4 g/株对烟株生长及丰产会造成不利影响,在苗期及大田期同时施用并不能改变这一结果.2）＂多肽保＂仅苗期施用量0.1～0.6 g/株,对烟草黑胫病的平均防效为60.12%,而常规对照防效为53.37%,若在苗期及大田期同时施用也几乎不能改变这一结果;苗期施用量达到或超过0.8 g/株会严重影响到防治效果.3）＂多肽保＂对烟草花叶病的防效,在苗期和大田期同时施用表现为叠加效应,其防效可达到93.44%,而常规对照的防效仅为61.7%;苗期施用量达到或超过0.2 g/株,在移栽时不施的情况下也能表现出良好的防效. 相似文献
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The AtTOM1 gene of Arabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus(TMV) in A.thaliana.In this study,we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1.Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N.tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities,respectively.Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N.benthamiana.The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants. 相似文献
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The AtTOM1 gene ofArabidopsis thaliana had been shown to be essential for the efficient multiplication of Tobacco mosaic virus （TMV） in A. thaliana. In this study, we cloned an AtTOM1-like gene from Nicotiana benthamiana named as NbTOM1. Sequence alignment showed that NbTOM1 is closely related to AtTOM1 homologues of N. tabacum and Lycopersicon esculentum with 97.2% and 92.6% nucleotide sequence identities, respectively. Silencing of NbTOM1 by a modified viral satellite DNA-based vector resulted in complete inhibition of the multiplication of TMV in N. benthamiana. The result suggests that inhibition of NbTOM1 via RNA silencing is a potentially useful method for generating TMV-resistant plants. 相似文献