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培养拔尖创新人才需要一流专业,而一流专业建设离不开创新型实验教学体系建设。以华中科技大学生命科学与技术学院生物物理学大实验课程为例,针对该领域商用教学设备少、缺乏针对性和前瞻性、滞后学科发展等问题,先后自制了膜片钳系统、视网膜电图仪、随机扫描光刺激系统以及小动物行为记录装置与光片式显微成像系统等教学仪器;并在此基础上开设了一系列新的实验项目,推进了学科的发展,使学生触及前沿技术,拓宽了实验的广度与深度,起到很好的教学效果,促进了实验室建设和实验教学改革,提升了学科专业特色和影响力。 相似文献
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We present a droplet-based microfluidic system for performing bioassays requiring controlled
analyte encapsulation by employing highly flexible on-demand droplet generation. On-demand droplet
generation and encapsulation are achieved pneumatically using a microdispensing pump connected to a
constant pressure source.
The system generates single droplets to the collection route only when the pump is actuated with a
designated pressure level
and produces two-phase parallel flow to the waste route during the stand-by state. We analyzed the effect of
actuation pressure on the
stability and size of droplets and optimized conditions for generation of stable droplets over a
wide pressure range. By
increasing the duration of pump actuation, we could either trigger a short train of identical size
droplets or generate a single larger droplet. We also investigated the methodology to control
droplet contents by fine-tuning flow rates or implementing a resistance bridge between the pump and main channels.
We demonstrated the integrated chip for on-demand mixing between two aqueous phases in droplets and
on-demand encapsulation of Escherichia coli cells. Our unique on-demand feature for
selective encapsulation is particularly appropriate for bioassays with extremely dilute samples,
such as pathogens in a clinical sample, since it can significantly reduce the number of empty
droplets that impede droplet collection and subsequent data analysis. 相似文献
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Acoustic radiation forces have been used to manipulate cells and bacteria in a number of recent microfluidic
applications. The net force on a cell has been subject to careful investigation over a number of decades. We
demonstrate that the radiation forces also act to deform
cells. An
ultrasonic standing
wave field is created in a
0.1 mm glass capillary at a frequency of 7.9 MHz. Using osmotically swollen red-blood cells, we show observable
deformations up to an aspect
ratio of 1.35, comparable to deformations created by optical tweezing. In contrast to optical technologies,
ultrasonic devices are
potentially capable of deforming thousands of cells simultaneously. We create a finite element model that includes both the acoustic
environment of the cell, and a model of the cell
membrane subject to forces resulting from the non-linear aspects of the acoustic
field. The model is found to
give reasonable agreement with the experimental results, and shows that the deformation is the result of variation in
an acoustic force that is directed outwards at all points on the cell membrane. We foresee applications in
diagnostic devices, and in the possibility of mechanically stimulating cells to promote differentiation and
physiological effects. 相似文献
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药食两用植物马齿苋植物学研究进展（2000～2010年） 总被引：1，自引：0，他引：1
王锦军 《荆门职业技术学院学报》2011,26(2):76-80
目的：便于对马齿苋植物开展深入研究,并为其开发利用提供背景知识参考。方法：常规文献检索并进行分类汇总。结果：获得2000～2010年有关马齿苋植物研究方面的原始研究文献52篇。国内外均有关于马齿苋营养成分与生态方面的研究,国外对其生物物理与生物化学有较多的研究而国内对其组织培养与栽培管理有较多涉及。结论：马齿苋是一味值得深入研究的药食两用植物,有广阔的商业开发前景。 相似文献
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种子萌发实验、砂培和土培试验结果表明,酸模、小头蓼和戟叶酸模对Cu具有较强的耐性和蓄积能力,在染Cu环境中能正常生长。砂培条件下,这些植物能在7 mg·L-1Cu处理的营养液中生长并蓄积Cu。小头蓼根部含Cu量明显高于酸模和戟叶酸模,前者平均含Cu量为(4765.06±484.28)mg·kg-1, 后两者分别为(1749.23±154.98)mg·kg-1、(890.26±57.21)mg·kg-1;茎叶部含Cu量也以小头蓼较高,平均为(214.04±16.31)mg·k-1,戟叶酸模和酸模较低,分别为(34.59±4.94)mg·kg-1、(27.59±5.32)mg·kg-1。土培试验也证实,酸模和戟叶酸模均能蓄积一定的Cu。据此推测,酸模、小头蓼和戟叶酸模均可作为植物修复铜污染土壤的先锋物种。 相似文献
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Panwong Kuntanawat Jirapat Ruenin Rungrueang Phatthanakun Phongsakorn Kunhorm Werasak Surareungchai Sompong Sukprasong Nimit Chomnawang 《Biomicrofluidics》2014,8(3)
A simple microwell-based microfluidic chip for microalgal cells trapping was
fabricated. An electrostatic
cell trapping
mechanism, enabled by a positively charged glass surface, was used. The chip was capable of capturing multiple algal
cell types.
In the case of filamentous Spirulina platensis, we observed single filament
occupancy of up to ∼30% available wells, as high as some previously proposed methods. Captured
filaments were not of any preferential size, suggesting well randomized cell trapping. It was found
that the electrostatic
attraction did not affect the cell
growth. Total replacement of liquid inside the wells could be achieved by pumping
new solutions via the inlet, making single cell experiments in controlled chemical conditions
possible. After the top layer of the chip was removed, cells in the wells could be simply transferred using a
micropipette, turning the chip into a platform for strain selection. 相似文献
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"择时健身"的提出与"同步生物节奏健身锻炼法"的确立——兼论时间生物学在健身锻炼中的应用 总被引：1，自引：0，他引：1
运用时间生物学、时间医学和运动时间学等理论,阐述了人的机体内环境存在的各种时间生物学规律(生物节奏),分析了内环境与外环境之间的时间学关联,及人在每日、每周、全年中机体各器官、系统和人的运动能力的生物节奏规律,合理的安排健身锻炼的时间、内容、手段、负荷和制定科学的健身运动处方,并据此确立了“择时健身”的新理念和“同步生物节奏锻炼法”的新方法,对提高身体锻炼效果有好处。 相似文献