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介绍一种分散固相离子交换萃取方法,结合毛细管电泳-激光诱导荧光检测法,测定血清样品中不对称二甲基精氨酸、对称二甲基精氨酸和单甲基精氨酸。将提取甲基化精氨酸的树脂粒加入到4-氯-7-硝基苯并呋喃乙腈溶液和碱性硼酸钠溶液（pH=10.5）的混合溶液中,于60℃水浴中衍生化。在80 mmol/L磷酸盐溶液（pH=2.0）分离电解质和20 kV分离电压条件下,这3种衍生物在熔融石英毛细管中获得基线分离。根据FDA生物分析方法验证指南,进行方法验证,结果满意。利用该方法测定了当地医院血清样品中的3种甲基化精氨酸含量。 相似文献
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Li L Le F Wang LY Xu XR Lou HY Zheng YM Sheng JZ Huang HF Jin F 《Journal of Zhejiang University. Science. B》2011,12(10):796-804
An association between assisted reproductive technology (ART) and neurobehavioral imprinting disorders has been reported in
many studies, and it seems that ART may interfere with imprint reprogramming. However, it has never been explored whether
epigenetic errors or imprinting disease susceptibility induced by ART can be inherited transgenerationally. Hence, the aim
of this study was to determine the effect of in vitro fertilization and embryo transfer (IVF-ET) on transgenerational inheritance
in an inbred mouse model. Mice derived from IVF-ET were outcrossed to wild-type C57BL/6J to obtain their female and male line
F2 and F3 generations. Their behavior, morphology, histology, and DNA methylation status at several important differentially
methylated regions (DMRs) were analyzed by Morris water maze, hematoxylin and eosin (H&E) staining, and bisulfite genomic
sequencing. No significant differences in spatial learning or phenotypic abnormality were found in adults derived from IVF
(F1) and female and male line F2 and F3 generations. A borderline trend of hypomethylation was found in H19 DMR CpG island 3 in the female line-derived F3 generation (0.40±0.118, P=0.086). Methylation status in H19/Igf2 DMR island 1, Igf2 DMR, KvDMR, and Snrpn DMR displayed normal patterns. Methylation percentage did not differ significantly from that of adults conceived naturally,
and the expression of the genes they regulated was not disturbed. Transgenerational integrity, such as behavior, morphology,
histology, and DNA methylation status, was maintained in these generations, which indicates that exposure of female germ cells
to hormonal stimulation and gamete manipulation might not affect the individuals and their descendents. 相似文献
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本文对引起环境污染状况加剧的主要原因进行了分析探讨，笔者并提出了个人的见解，认为人类活动中的某些不规范、不科学的行为是引起问题的关键所在，而导致环境问题加剧的直接诱因，则是环境中金属元素及其物种的大量增加。 相似文献
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众所周知 ,DNA是遗传信息的携带者 ,遗传现象主要是由基因决定的。但是 ,近几年来科学家发现 ,朊病毒是一种可遗传的蛋白质粒子 ;DNA甲基化也导致一些遗传现象的发生 ;性状间的相互制约也可引起不受基因控制的表型出现……这些现象说明DNA并非遗传的全部。 相似文献
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