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阿布来提·阿布都热西提 《喀什师范学院学报》2011,32(6):43-45
用乙醇热回流提取法对阿里红中总黄酮进行了提取,通过分光光度法,以芦丁为对照品,测定了阿里红中总黄酮含量.本法简便,快速,可用于阿里红生药及其制剂总黄酮含量测定. 相似文献
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巴戟天多糖对体外培养兔软骨细胞增殖的影响 总被引:4,自引:0,他引:4
如何能够促进软骨细胞的增殖是研究防治骨性关节炎(Osteoarthritis,OA)的重大课题.采用体外培养兔软骨细胞的方法,在培养过程中加入巴戟天多糖;实验结果证实巴戟天多糖能够促进软骨细胞增殖,以1.25 μg/mL的浓度为最优,巴戟天多糖促进软骨细胞的增殖作用可能与其使细胞增殖核抗原(PCNA)的表达增加有关. 相似文献
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Vasudha Shukla Manish Vashistha Som Nath Singh 《Indian journal of clinical biochemistry : IJCB》2009,24(1):70-75
Aqueous and alcoholic extracts of amalki (Emblica officinalis), spirulina and wheatgrass were prepared and analyzed for antioxidant
vitamin content (vitamin C and E), total phenolic compounds. Antioxidant status, reducing power and effect on glutathione
S-transferase (GST) activity were evaluated in vitro. Vitamin C content of crude amalaki powder was found to be 5.38 mg/g,
while very less amount 0.22 mg/g was detected in wheat grass. Amalki was rich in vitamin E like activity, total phenolic content,
reducing power and antioxidant activity. Total antioxidant activity of aqueous extract of amalki, spirulina and wheat grass
at 1mg/ml concentration were 7.78, 1.33 and 0.278 mmol/l respectively. At similar concentrations the total antioxidant activity
of alcoholic extract of amalaki, spirulina and wheat grass was 6.67, 1.73 and 0.380 mmol/l respectively. Amalki was also found
to be rich source of phenolic compounds (241mg/g gallic acid equivalent). Alcoholic extract of wheat grass showed 50 % inhibition
in FeCl2- ascorbic acid induced lipid peroxidation of rat liver homogenates in vitro. Both aqueous and alcoholic extracts of amalaki
inhibited activity of rat liver glutathione S-transferase (GST) in vitro in dose dependant manner. Since GST acts as powerful
drug metabolizing enzyme its inhibition by amalaki offers possibility of its use for lowering therapeutic dose of herbal preparations.
The aqueous extracts of both amalki and spirulina also showed protection against t-BOOH induced cytotoxicity and production
of ROS in cultured C6 glial cells. 相似文献
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以海枫藤的茎为实验材料,用乙醇和水进行提取浓缩获得海枫藤茎提取物(MOSE).通过研究MOSE对昆明种小鼠体外精子活性的影响,证实MOSE在体外具抗生育作用,其抗生育活性存在剂量依存关系.这表明海枫藤茎中含有具抗生育能力的活性物质并具明显抗生育功效,该物质主要为醇溶性物质. 相似文献
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B. Antony M. Benny T. N. B. Kaimal 《Indian journal of clinical biochemistry : IJCB》2008,23(4):378-381
Emblica officinalis Gaertn., commonly known as the Indian gooseberry or “Amla”, has been used as health food for centuries
in India and other Asian countries. The biological effects of amla have been attributed to the antioxidant properties of the
low-molecular weight hydrolysable tannins present in the fruit. Amlamax™ is a purified, standardized, dried extract of amla
containing about 35% galloellagi tannins along with other hydrolysable tannins. Our earlier studies on rabbits showed significant
reduction in total cholesterol and triglycerides as well as increase in HDL. The present study extends these results to human
volunteers. Two doses of the extract were evaluated — 500 mg and 1000 mg per day for 6 months. Blood samples were collected
at the 3rd and 6th months showed reduction in total and LDL cholesterols and enhancement of beneficial HDL cholesterol. In
addition, blood CRP levels, a marker for inflammation, were also significantly reduced. Since dyslipidemia and inflammation
are the two major components of cardiovascular diseases, the present results must be considered encouraging and indicate the
potential of Amlamax™ in the management of heart diseases. 相似文献
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Damodara Reddy V Padmavathi P Gopi S Paramahamsa M Varadacharyulu NCh 《Indian journal of clinical biochemistry : IJCB》2010,25(4):419-424
The effect of Emblica officinalis fruit extract (EFE) against alcohol-induced hepatic damage in rats was investigated in the present study. In vitro studies showed that EFE possesses antioxidant as well nitric oxide (NO) scavenging activity. In vivo administration of alcohol (5 g/kg b.wt/day) for 60 days resulted increased liver lipid peroxidation, protein carbonyls, nitrite plus nitrate levels. Alcohol administration also significantly lowers the activities of superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase and reduced glutathione as compared with control rats. Administration of EFE (250 mg/kg body weight) to alcoholic rats significantly brought the plasma enzymes towards near normal level and also significantly reduced the levels of lipid peroxidation, protein carbonyls and restored the enzymic and non-enzymatic antioxidants level. This observation was supplemented by histopathological examination in liver. Our data indicate that the tannoid, flavonoid and NO scavenging compounds present in EFE may offer protection against free radical mediated oxidative stress in rat hepatocytes of animals with alcohol-induced liver injury. 相似文献
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采用同时蒸馏萃取法提取海枫藤挥发性成分,并用气相色谱—质谱法对提取物进行化学成分分析.鉴定出46个成分,以归一化法测定了各个成分的质量分数.其中超过8%的有4种:亚油酸(8.26%),(Z,Z,Z)-9,12,15-十八碳三烯-1-醇(9.63%),N-苯基苯胺(12.00%),棕榈酸(29.49%). 相似文献
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从植物学特征及生物学特性等方面,对彝族药用植物海枫藤进行调查和试验.研究结果表明:海枫藤是一种具有很好观赏、绿化、药用价值和纤维用途等方面开发价值的重要资源植物.通过对海枫藤的形态特征、生物学特性、植株分布和伴生植物等调查分析,为开发利用和保护海枫藤提供科学依据. 相似文献
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研究迷迭香对大强度耐力训练大鼠血清睾酮和肾上腺皮质激素的影响。方法:本实验选用SD大鼠,建立大强度耐力训练模型,训练结束测定其血清睾酮和肾上腺皮质激素。结果:大强度耐力训练可使大鼠血清睾酮下降,皮质醇水平上升,睾酮/皮质醇(T/C)值下降,说明大强度耐力训练已造成大鼠机体神经内分泌系统机能紊乱,使大鼠体内的分解代谢持续加强。服用迷迭香后大鼠血清睾酮水平均明显高于其相应的对照组,T/C值明显升高;服用迷迭香的大强度耐力训练组大鼠血清皮质醇水平明显下降。结论:服用迷迭香可以促进大强度耐力训练后大鼠合成代谢加强,有利于能量物质的储存,促进运动性疲劳的恢复。 相似文献