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Jin-ting YANG Li-hui TANG Yun-qing LIU Yin WANG Lie-ju WANG Feng-jiang ZHANG Min YAN 《Journal of Zhejiang University. Science. B》2015,16(5):395-403
目的:研究离体热处理和顺铂联合热处理对术中回收血红细胞功能的影响及其中混杂的肝肿瘤细胞株(HepG2)的杀灭作用。创新点:(1)采用多种评价指标研究了不同时间离体热处理对术中回收血中混杂的HepG2的杀灭作用及对红细胞的影响,并确定了对红细胞安全且能有效杀灭HepG2的离体热处理时间。(2)从多个角度评价了离体顺铂联合热处理对术中回收血红细胞的影响及对其中混杂的HepG2的杀灭作用,确定了该方案中对红细胞安全且能有效去除HepG2的顺铂浓度。方法:采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)、5-乙炔基-2’脱氧尿嘧啶核苷(EdU)和平板克隆形成评估HepG2的细胞存活率、DNA复制率和克隆形成能力(图1和3);从红细胞渗透脆性、携氧能力(2,3-二磷酸甘油酸(2,3-DPG)、半饱和氧分压(P50))、能量代谢(Na+-K+-ATPase、pH)、膜完整性(游离血红蛋白(Hb)、血清K+和Na+浓度、细胞膜磷脂酰丝氨酸外翻比例)等角度评估红细胞功能(图2和4;表3)。结论:肝肿瘤术中回收血经离体顺铂联合热处理(42°C,50μg/ml)60min后,能有效清除其中混杂的HepG2,但对红细胞无显著影响,值得体内进一步研究顺铂热处理有效应用于肿瘤手术自体血液回输的安全方案。 相似文献
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Hemolysis of erythrocytes from human and porcine blood was studied at different calcium chloride (CaCl2) concentrations (0.04–1.83 mM) and at a constant bilirubin concentration (72 mM). Although, human erythrocytes showed significant
hemolysis (64%) at the highest CaCl2 concentration (1.83 mM) used in this study, remarkable resistance to this phenomenon was observed with porcine erythrocytes
as only 11% hemolysis was observed. A similar pattern in the behavior of both human and porcine erythrocytes was observed
when parameter such as bilirubin concentration or time of incubation with bilirubin was varied. Other divalent cations such
as Mn2+, Ba2+ and Mg2+ were either least effective or ineffective in inducing hemolysis in presence of bilirubin. Serum albumin played a protective
role in this phenomenon in a concentration dependent manner, as no hemolysis was observed at a bilirubin/albumin molar ratio
of 1:1 or less. Differences in the structural make-up of proteins and lipids in the erythrocyte membranes of the two species
may account for their different behavior. 相似文献
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Harbans Lal Nirmal Gulati Saroj Nidhi Sandooja Kiran Chugh 《Indian journal of clinical biochemistry : IJCB》1995,10(2):103-105
Plasma and erythrocyte magnesium levels were estimated in preeclampsia and eclampsia. Mean plasma as well as erythrocyte magnesium
concentrations were found to be significantly lowered than the controls. Hypomagnesemia was directly related with the severity
of the disease. Plasma calcium concentration however, was not significantly altered. 相似文献
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