排序方式： 共有6条查询结果，搜索用时 15 毫秒
1
1.
碳纤维增强型树脂基复合材料的生物安全性评价 总被引：3，自引：0，他引：3
目的评价碳纤维增强型树脂基复合材料(CFRP)的生物相容性,为其在口腔临床中的应用提供依据。方法本研究进行三种体内外试验,即短期全身毒性试验(经口途径),静脉注射急性全身毒性试验和溶血试验。结果实验一:对照组和试验组大鼠均未出现毒性反应,对照组和实验组的体重增长率和食物利用率无明显差异(P>0.05),肉眼观察动物重要脏器,均未出现病理变化;实验二:对照组和试验组小鼠均未出现毒性症状,试验组和对照组小鼠体重无明显差异(P>0.05);实验三:测的体外溶血率2%。结论碳纤维增强型树脂基复合材料对动物无全身毒性作用也无溶血作用。 相似文献
2.
In order to assess the possible occurrence of acute haemolysis with prolonged exertion, serum haptoglobin levels were determined from venous blood samples collected from eight male runners immediately preceding (PreRH), immediately following (PRH1), and 6 h following (PRH2) completion of a marathon road race. The subjects’ mean age, percentage of body fat, and maximum oxygen uptake (VO2max) were 46 ± 9 years, 12.1 ± 3.4% and 54.9 ± 8.4 ml kg‐1 min‐1, respectively. The mean race finish time for the subjects was 3:35 ± O: 18 h:min. The PreRH, PRH1 and PRH2 averaged 129 ± 18, 97 ± 48 and 86 ± 35 mg dl‐1 respectively. Significant differences of –32.5 mg dl‐1 between PreRH versus PRH1 and –42.5 mg dl‐1 between PreRH versus PRH2 were found. The difference between PRH1 and PRH2 of –10.6 mg dl‐1 was not significant. No significant correlations were found between the decreases in serum haptoglobin and VO2max or race finish time. The data suggest the occurrence of an acute haemolysis with performance of the marathon road race. 相似文献
3.
Rufino Mondejar María Mayor Reyes Enrique Melguizo Madrid Consuelo Caavate Solano Santiago Prez Ramos 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2021,31(2)
IntroductionTotal bilirubin tests are highly demanded in clinical laboratories. Since icteric index (I-index) has zero cost, we aimed to evaluate its clinical utility and cost-effectiveness to determine if total bilirubin is necessary to be tested. We took into account if haemolysis could interfere to icteric index determination.Material and methodsRetrospectively we reviewed I-index results in two cohorts (43,372 and 8507 non-haemolysed and haemolysed samples, respectively). All determinations were done using Alinity c chemistry analysers (Abbott Diagnostics). Receiver operating characteristic (ROC) curve was used to determine the optimal index cut-off to discriminate between normal and abnormal bilirubin concentration (20.5 µmol/L).ResultsThe ROC curve analysis suggested 21.4 µmol/L as the optimal I-index cut-off but differences in sensitivity and specificity were detected between patient derivation. For rejecting purpose, 15.4 µmol/L and 17.1 µmol/L I-index thresholds were selected based on patient derivation (inpatients and emergency room; and primary care and outpatients, respectively) with 97% sensitivity and 0.25% false negative results. Sensitivity was much lower in haemolysed samples. We selected 34.2 µmol/L I-index as threshold to detect hyperbilirubinemia with 99.7% specificity and 0.26% false positive results, independent of haemolysis. With the icteric index cut-offs proposed, we would save 66% of total bilirubin requested and analyse total bilirubin in around 2% of samples without total bilirubin requested.ConclusionsThis study supports the use of I-index to avoid bilirubin determination and to identify patients with hyperbilirubinemia. This work considers that the economic and test savings could help to increase the efficiency in clinical laboratories. 相似文献
4.
Abdulkadir at Kamil Taha Uar Alper Gümü 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2021,31(1)
IntroductionWe investigated the interference of haemolysis on ethanol testing carried out with the Synchron assay kit using an AU680 autoanalyser (Beckman Coulter, Brea, USA).Materials and methodsTwo tubes of plasma samples were collected from 20 volunteers. Mechanical haemolysis was performed in one tube, and no other intervention was performed in the other tube. After centrifugation, haemolysed and non-haemolysed samples were diluted to obtain samples with the desired free haemoglobin (Hb) values (0, 1, 2, 5, 10 g/L). A portion of these samples was then separated, and ethanol was added to the separated sample to obtain a concentration of 86.8 mmol/L ethanol. After that, these samples were diluted with ethanol-free samples with the same Hb concentration to obtain samples containing 43.4, 21.7, and 10.9 mmol/L. Each group was divided into 20 equal parts, and an ethanol test was carried out. The coefficient of variation (CV), bias, and total error (TE) values were calculated.ResultsThe TE values of haemolysis-free samples were approximately 2-5%, and the TE values of haemolysed samples were approximately 10-18%. The bias values of haemolysed samples ranged from nearly - 6.2 to - 15.7%.ConclusionsHaemolysis led to negative interference in all samples. However, based on the 25% allowable total error value specified for ethanol in the Clinical Laboratory Improvement Amendments (CLIA 88) criteria, the TE values did not exceed 25%. Consequently, ethanol concentration can be measured in samples containing free Hb up to 10 g/L. 相似文献
5.
Müjgan Ercan Emi Deniz Akbulut Nihayet Bayraktar erif Ercan 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2021,31(3)
IntroductionThe current study aimed to assess the interference of in vitro haemolysis on complete blood count (CBC) using Abbott Alinity hq system, and to determine which haemolysis levels affect the reliability of sample results.Materials and methodsBlood samples obtained from 25 volunteers in K3-EDTA tubes were divided into four aliquots. The first aliquot was not subjected to any intervention. The second, third and fourth aliquots were passed through a fine needle 2, 4 and 6 times, respectively. Complete blood count was performed by multi-angle polarized scatter separation technology and haemolysis index (HI) was assessed from the plasma samples separated by centrifugation. Five groups were formed according to the HI values. The percentage biases between the results of non-haemolysed and haemolysed groups were compared with the desirable bias limits from The European Federation of Clinical Chemistry and Laboratory Medicine database and reference change values (RCVs).ResultsIn groups 1 to 4, the effects of haemolysis on CBC parameters were acceptable comparing to the analytical bias except for lymphocytes (7.26%-7.42%), MCH (2.59%), and MCHC (0.47%-2.81%). Results of group 5 (gross haemolysis) showed decreases in HCT(- 4.56%), RBC (- 4.07%) count and increase in lymphocyte (11.60%) count higher than the analytical performance specifications. Moreover, variations in MCH (4.65%) and MCHC (5.24%) were exceeding the RCVs.ConclusionsGross haemolysis (haemoglobin concentration > 10 g/L) is likely to produce unreliable CBC results on non-pathological samples. Further studies including pathological specimens are needed. 相似文献
6.
Chethana Chetan Ashok Prabhu Vivian D'Souza Shrinivas B. Rao 《Indian journal of clinical biochemistry : IJCB》2000,15(1):17-21
Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against
the peroxidative action of H2O2 which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations
used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H2O2 and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all
the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress
haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines
may have independent mechanism of action. 相似文献
1