尿酸氧化酶的研究及其应用   总被引：1，自引：0，他引：1  

开雷  岳珂  马晓航 《科技通报》2008,24(2):183-187

随着对血液中尿酸含量影响的研究发现,控制尿酸含量对于治疗一些疾病有着重要的作用,而尿酸氧化酶作为有效的控制尿酸含量的治疗试剂越来越受到关注。本文综述了尿酸氧化酶的来源、结构、酶学性质以及当前尿酸氧化酶在临床上应用的研究,并对尿酸氧化酶研究和应用中存在的问题和今后研究的发展做了展望。  相似文献   

Quantification of urinary urate with alkylamine glass-bound uricase and arylamine glass-bound peroxidase     

C. S. Pundir  A. K. Bhargava 《Indian journal of clinical biochemistry : IJCB》2003,18(1):22-28

Uric acid in urine was analyzed using porcine liver uricase and horseradish peroxidase immobilized on alkylamine and arylamine glass beads (pore diameter 55nm) respectively. The minimum detection limit was 5.0mg/0.1 ml urine. The recovery of added uric acid was 92%. Within and between assay CVS were <1.3% and <5.3% respectively. A good correlation (r=0.93) was found between urinary urate values obtained by a commercial kit method and the present method. The uric acid in 24hr urine of apparently healthy adults and person sufering from various diseases was found to be 450 to 900mg/24hr, 659mg/24hr (range, mean) and 910–1400mg/24hr, 1145 mg/24hr (range, mean) respectively with the present method.  相似文献   

Science Letters：Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method   总被引：4，自引：0，他引：4  

Liao F  Zhao YS  Zhao LN  Tao J  Zhu XY  Liu L 《Journal of Zhejiang University. Science. B》2006,7(6):497-502

A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action （A0） was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance （Ab） was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from AA, the difference between A0 and Ab, using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV〈4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine 〈0.32 mmol/L in serum had no significant effects. △A linearly responded to 1.2 to 37.5 μmol/L uric acid in reaction solution containing 15 μl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By Bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost.  相似文献