Effects of osmotic stress on antioxidant enzymes activities in leaf discs of P<Subscript>SAG12</Subscript>-<Emphasis Type="Italic">IPT</Emphasis> modified gerbera     

Lai QX  Bao ZY  Zhu ZJ  Qian QQ  Mao BZ 《Journal of Zhejiang University. Science. B》2007,8(7):458-464

Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.  相似文献   

浅谈微博在尿沉渣形态学教学改革中的作用     

李小龙  陶洪群  胡昕  郑晓群 《科教文汇》2020,(5):77-79

伴随医学检验专业改制(五年学制改为四年制),教学学时也相应缩减,在此形势下,如何保持教学质量尤其是尿沉渣形态学教学质量成为目前教改的焦点。利用微博建立尿沉渣形态学线上教学互动平台就可解决这一问题,教改实践证明:这样不仅保持了教学质量,学生对形态学的学习兴趣也提高了。  相似文献   

SYSMEX UF-1000i尿有形成分分析仪对尿路感染的诊断价值     

战伟 《大连大学学报》2015,(3):100-102

采用Sysmex UF-1000i尿有形成分分析仪和AX-4280尿干化学分析仪测定尿标本,同时进行尿细菌定量培养。把测定值与尿培养金标准比对,干化学法白细胞酯酶和UF-1000i细菌计数结果的一致性适中,与亚硝酸盐结果的一致性较低,与尿白细胞计数结果的一致性极高。细菌定量培养的特异性、灵敏度及一致性高于UF-1000i白细胞计数。UF-1000i在临床快速诊断尿路感染时应用价值较高。  相似文献   

急性耐力运动对大鼠脑组织一氧化氮和一氧化氮合成酶的影响及中药调节作用的研究   总被引：3，自引：1，他引：2  

宋亚军  马伟  郝梅 《体育科学》2004,24(7):25-27

采用实验法，探讨一次性长时间运动后及恢复期大鼠脑组织一氧化氮(NO)和一氧化氮合成酶(NOS)的变化以及中药对其调节性作用。研究结论：急性耐力运动后大鼠端脑iNOS活性显著性增高、cNOS显著性降低，而NO水平的过量提高可能具有神经毒性作用，这可能是急性耐力运动引发运动性中枢疲劳的重要原因；急性耐力运动后20h恢复期，大鼠端脑NO及其合成酶系的变化趋势与病理性脑损伤存在明显差异，这可能与急性耐力运动中脑组织L-精氨酸分解代谢加速和脂质过氧化水平提高有关；中药营养补充对减缓急性耐力运动中NO过量增高、保护脑组织、推迟中枢疲劳的产生具有一定的积极性作用。  相似文献   

Effect of arogh—A polyherbal formulation on the marker enzymes in isoproterenol induced myocardial injury     

S. Suchalatha  C. S. Shyamala Devi 《Indian journal of clinical biochemistry : IJCB》2004,19(2):184-189

To study the protective role of Arogh on isoproterenol induced myocardial damage in rats. The effect of Arogh pretreatment on isoproterenol induced myocardial damage was assessed by studying the levels of lipid peroxides and changes in the activity of marker enzymes such as creatine kinase, lactate dehydrogenase and transaminases in serum and heart tissue. In isoproterenol administered rats, a significant decrease was observed in the activity of marker enzymes in the heart with a corresponding increase in their levels in serum. Lipid peroxide levels increased significantly in the serum and heart. In rats pretreated with arogh, the level of lipid peroxides and the activity of marker enzymes were maintained at near normal values. Pretreatment with Arogh offered a protective effect against isoproterenol induced myocardial damage in rats as evidenced by LDH isoenzyme patterm and histopathological studies of heart tissue.  相似文献   

Enzymuria pattern in early post renal transplant period: Diagnostic usefulness in graft dysfunction     

Banibrata Mukhopadhyay  Shashikant Chinchole  Valentine Lobo  Sishir Gang  Mohan Rajapurkar 《Indian journal of clinical biochemistry : IJCB》2004,19(2):14-19

Serum creatinine does not distinguish between various causes of graft dysfunction. Serial assay of proximal tubular enzymes N-Acetyl-D-glucosaminidase (NAG), Alanine aminopeptidase (AAP) and Gamma glutamyl transferase (GGT) in urine was done to assess their usefulness in distinguishing various causes of graft dysfunction. Daily serum creatinine and enzymuria were measured in 32 consecutive renal allograft recipients for first 15 postoperative days. Graft dysfunction was defined as >20% increase in serum creatinine and >100% increase in enzymuria over the baseline. The diagnosis of graft dysfunction was based upon clinical criteria, ultrasonography, cyclosporin trough level, allograft biopsy, response to anti-rejection therapy and alteration of cyclosporin dosage. Fifteen episodes of graft dysfunction were identified in 15 patients. The sensitivity and specificity of the enzymes (NAG, AAP and GGT) for predicting graft dysfunction were 87.5%, 86.9%, 88.5% and 98.2%, 98.2%, 97.9% respectively. There was a significant increase in enzymuria during acute tubular necrosis (ATN) and acute rejection episode compared to cyclosporin nephrotoxicity (p<0.01). Enzymuria assay provides a simple, reliable and noninvasive method to distinguish cyclosporin nephrotoxicity from acute tubular necrosis and acute rejection in renal allograft recipients.  相似文献   

Mechanism of Antidiabetic Action of Compound GII Purified from Fenugreek (<Emphasis Type="Italic">Trigonella foenum graecum</Emphasis>) Seeds     

D. Puri  K. M. Prabhu  G. Dev  S. Agarwal  P. S. Murthy 《Indian journal of clinical biochemistry : IJCB》2011,26(4):335-346

To study the mechanism of action of water soluble compound GII purified from fenugreek (Trigonella foenum graecum) seeds which was shown earlier to have antidiabetic effect in the subdiabetic, moderately and severely diabetic rabbits. In rabbits (1–1.5 kg bw) diabetes was induced by intravenous injection of 80 mg/kg bw of alloxan. They were fed with GII at a dose of 50 mg/kg bw daily once in the morning for 15 days in the subdiabetic and moderately diabetic and 30 days in the severely diabetic rabbits. Serum total cholesterol (TC), triglycerides (TG), LDL + VLDL cholesterol [(LDL + VLDL)C], HDL cholesterol [(HDL)C], total tissue lipids, glycogen and enzymes of carbohydrate metabolism (glycolysis, gluconeogenesis, polyol pathway) hexokinase, glucokinase, pyruvate kinase, malic enzyme, glucose-6-phosphatase, glucose-6-phosphate dehydrogenase, aldose reductase and sorbitol dehydrogenase and antioxidant enzymes glutathione peroxidase, glutathione reductase and superoxide dismutase were estimated. Liver and kidney function parameters were also estimated. Treatment with GII for 15 days in the subdiabetic and moderately diabetic rabbits and for 30 days in the severely diabetic rabbits (i) decreased the elevated lipids TC, TG, (LDL + VLDL)C and increased the decreased (HDL)C, (ii) decreased the elevated liver and heart total lipids, TC and TG, (iii) increased the decreased liver and muscle glycogen, (iv) increased the decreased hexokinase, glucokinase, pyruvate kinase, malic enzyme, glucose-6-phosphate dehydrogenase, superoxide dismutase, glutathione peroxidase, (v) decreased the increased glucose-6-phosphatase, sorbitol dehydrogenase, aldose reductase. Results thus show that treatment with GII compound purified from fenugreek seeds for 15 days in the subdiabetic and moderately diabetic and 30 days in the severely diabetic rabbits corrects the altered serum lipids, tissue lipids, glycogen, enzymes of glycolysis, gluconeogenesis, glycogen metabolism, polyol pathway and antioxidant enzymes. Histopathological abnormalities (fatty infiltration and other cellular changes) seen in the pancreas, liver, heart and kidneys were repaired after treatment with GII. In fact partially damaged pancreas was repaired. Liver and kidney function test results were normal in the GII treated animals indicating that GII treatment is safe and free from any side effects.  相似文献   

帕金森病患者下尿路功能障碍治疗研究进展     

吴志刚  沈丽霞 《河北北方学院学报(医学版)》2014,(4):36-41

帕金森病（Parkinson’sdisease,PD）是一种渐进的中枢神经系统退行性疾病。尽管帕金森病是以运动症状为主要特征,但其非运动症状也逐渐受到重视。帕金森病患者常常会出现下尿路功能障碍,主要症状包括尿急、尿频、夜尿症、急迫性尿失禁等,严重影响患者的生活质量。该文综述了目前临床常用的治疗策略以及一些潜在的治疗方法。  相似文献   

女子举重运动员比赛期间的心电图及尿蛋白的变化     

王人卫  胡贤豪  柯松儿  诸剑英 《上海体育学院学报》1992,(3)

本文运用检测方法,对女子举重运动员比赛期间的心电图、尿蛋白及血红蛋白等变化进行测试与分析。结果表明,女子举重运动员在比赛期间,心室壁没有增厚;尿蛋白变化较大,其排出量有性别差异;血红蛋白浓度变化不大。  相似文献   

温度、pH对胡子鲶主要消化酶活性的影响     

黄周英  陈怀宇  洪淑婷  郑培蓉 《泉州师范学院学报》2008,26(6):86-90

研究胡子鲶胃、前肠、中肠、肝胰脏四个部位的主要消化酶（蛋白酶、淀粉酶、脂肪酶）活性以及温度、pH对其的影响．结果表明：在四个部位中,蛋白酶活力由高到低依次为：胃〉前肠〉肝胰脏〉中肠;淀粉酶活力由高到低依次为：肝胰脏〉中肠〉胃〉前肠;脂肪酶活力由高到低依次为：前肠〉中肠〉胃〉肝胰脏．胃蛋白酶活性最高,其适宜温度为30-35℃。适宜pH为5．5;肝胰脏淀粉酶活性最高,其适宜温度为25~35℃。适宜pH为6．0～7．0;前肠脂肪酶活性最高,其适宜温度为35-40℃,适宜pH为7．0～7．5．  相似文献