被子植物精细胞分离和特性研究进展     

朱维红  张妍  崔彬彬 《保定学院学报》2020,(3):114-121

精细胞分离是研究雄性生殖发育的前提之一,近半个世纪以来,人们运用不断更新的生物实验技术,对被子植物精细胞开展了分离及细胞分子特征的研究。分析被子植物精细胞分离方法,总结精细胞二型性与倾向性、细胞融合方面的研究成果,并对其研究意义和前景进行讨论。  相似文献   

激流回旋运动员神经类型特征及竞赛心理素质的调查与分析   总被引：2，自引：0，他引：2  

杨海平 《广州体育学院学报》2003,23(6):53-54,80

本文主要采用测验法对20名激流回旋运动员的神经类型及竞赛心理素质进行了调查与分析。结果表明:神经类型分布为:灵活型、亚灵活型为10％;亚稳定型为16人,占总人数的80％;亚易扰型为10％;在认知状态焦虑、状态自信心与水上运动常模组经t检验存在非常显著的差异(t=6.601,p<0.01;t=7.557,p<0.01),躯体状态焦虑与水上运动常模组经t检验差异不显著(t=0.244,p>0.05)。主要结论:激流回旋运动员神经类型,亚稳定型居多;运动员认知状态焦虑偏低,躯体状态焦虑适宜,状态自信心偏高;在责任感、抱负水平、应付困境能力得分较高,具备了取得优异成绩的心理能力。  相似文献   

口外翼下颌阻滞麻醉的应用解剖学研究     

蒋康  刘亚国 《大连大学学报》1992,(3)

在40侧头预部正中矢状切开标本和60侧下颌骨标本上,按口外翼下颌阻滞麻醉的设计要求,解剖观察了下牙槽神经、舌神经在翼下颌间隙的位置。测量了从下颌管下缘点和下颌角行口外翼下颌阻滞麻醉时的进针深度和角度。测得从下颌骨下缘点的进针深度为37mm;下颌角点的深度为30mm,穿刺针与下颌支后缘之间的角度为31°。对采用该法行口外阻滞麻醉的解剖学要点,优缺点进行了讨论。  相似文献   

In vitro study on ribozyme against hepatitis C virus     

Chen Zhi  Liu Yong  Liu Ke-zhou  R. H. Dennin  Dou Jun  U Reinhard 《浙江大学学报(A卷英文版)》2001,2(1):100-103

Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region, were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%, respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion: Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better effect when used together.  相似文献   

水稻胚乳细胞发育与结实特性     

黄升谋 《襄樊学院学报》2011,32(8):24-27

文章对水稻胚乳细胞发育过程、淀粉体和蛋白体结构特征与结实特性、养分输入胚乳细胞的途径、ATPase活性分布与水稻充实的关系、水稻强弱势粒胚乳细胞发育与其结实特性进行分析.提出进一步研究胚乳细胞发育及其超微结构与籽粒生理活性的关系,可从深入开展亚显微水平下强弱势粒背部维管束结构、ATPase的活性分布、差异表达基因对结实特性的影响等方面展开研究.  相似文献   

生物电信号激励与探测用微电极阵列     

潘海仙  吕晓迎  王志功  方涛  邱雷  黄宗浩 《东南大学学报》2011,(4):361-366

介绍了一种微电极阵列(MEA),该MEA包含60个独立的电极,其中59个为工作电极,一个为参考电极.工作电极成对排列,每一对工作电极分别包含一个激励电极和一个探测电极.结合外围电路,每一对工作电极的组合都可以实现对电极表面培养的生物组织或切片进行4种状态(激励、探测、同时激励和探测、隔离)的控制.MEA采用PCB工艺制...  相似文献   

Mapping the knowledge structure exhibited by a cohort of students based on their understanding of how a galvanic cell produces energy          下载免费PDF全文

Adrian Sin Loy Loh  R. Subramaniam 《科学教学研究杂志》2018,55(6):777-809

This study evaluates the use of an open‐ended question to determine students' knowledge structure on the topic of galvanic cells. The open‐ended question was developed and administered to 163 Grade 10 students who had earlier completed a course on electrochemistry. Students' responses were marked as well as coded on the basis of the fields identified from their responses. This was then evaluated statistically to determine the collective knowledge structure of the sample of students. The knowledge structure thus mapped contains both canonical concepts and alternative conceptions (ACs). An important finding emerging from this study is that instructors need to focus student's attention on the dynamic processes involving electrons and ions during the operation of galvanic cells. In order for students to fully understand how a galvanic cell operates, they need to see the whole picture. There are three critical components that lead to students' understanding of how an oxidation–reduction reaction can generate energy and how a circuit is complete: transfer of electrons during oxidation–reduction half‐reactions, flow of electrons within metals, and migration of ions in solution. Also, we found that it is possible for students to use correct chemistry concepts in an incorrect way by establishing linkages among these in an inappropriate manner. We reiterate that apart from evaluating students' ACs, it is also important to evaluate the links between the concepts and conceptions present in students' knowledge structure so that teaching can be made more effective.  相似文献   

Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart     

王建安  李长岭  樊友启  何红  孙勇 《浙江大学学报(A卷英文版)》2004,5(10):1279-1285

Objective: To investigate the directed transplantation of allograftic bone marrow-derived mesenchymal stem cells (MSCs) in myocardial infarcted (MI) model rabbits. Materials and Methods: Rabbits were divided into 3 groups, heart infarcted model with MSCs transplanted treatment (MSCs group, n=12), heart infarcted model with PBS injection (control group, n=20), sham operation with PBS injection (sham group, n=l 7). MSCs labelled by BrdUrd were injected into the MI area of the MSCs group. The same volume of PBS was injected into the MI area of the control group and sham group. The mortality, LVIDd, LVIDs and LVEF Of the two groups were compared 4 weeks later. Tropomyosin inhibitory component (Tn I) and BrdUrd immunohistochemistry identified the engrafted cells 4 weeks after transplantation. Result: The mortality of the MSCs group was 16.7% (2/12), and remarkably lower than the control group's mortality [35% (7/20) (P＜0.05)].Among the animals that survived for 4 weeks, the LVIDd and LVIDs of the MSCs group after operation were 1.17±0.21 cm and 0.74±0.13 cm, and remarkably lower than those of the model group, which were 1.64±0.14 cm and 1.19±0.12 cm (P＜0.05); the LVEF of the MSCs group after operation was 63±6%, and remarkably higher than that of the model group,which was 53±6% (P＜0.05). Among the 10 cases of animals that survived for 4 weeks in the MSCs group, in 8 cases (80%),the transplanted cells survived in the non MI, MI region and its periphery, and even farther away; part of them differentiated into cardiomyocytes; in 7 cases (70%), the transplanted cells participated in the formation of blood vessel tissue in the MI region. Conclusion: Transplanted allograftic MSCs can survive and differentiate into cardiomyocytes, form the blood vessels in the MI region. MSCs transplantation could improve the heart function after MI.  相似文献   

Effects of RNA interference-induced tryptase down-regulation in P815 cells on IL-6 and TNF-alpha release of endothelial cells     

Jiang YF  Zhao FD  Li XB  Ning YX  Zhi XL  Qian RZ  Yin LH 《Journal of Zhejiang University. Science. B》2008,9(8):656-661

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何杰金氏病肿瘤细胞生物学特征研究     

肖红  张恒  牟光玉 《遵义师范学院学报》2004,6(3):59-60,67

目的对何杰金氏病肿瘤细胞进行形态学观察和免疫表型研究.材料方法福尔马林固定,石蜡包理的何杰金氏病组织12例(NSHL6例,MCHL5例,LDHL1例)进行HE染色和CD30、CD15、CD20、CD45RO、Ki-67抗原免疫组化在肿瘤细胞和背景成分中的表达情况.结果(1)R-S细胞有各种变体形式.(2)各型HL具有不同的背景成分和组织学特征.(3)NSHL肿瘤细胞表达CD30(6/6)、CD15(1/6),不表达CD20(0/6).MCHL肿瘤细胞表达CD30(5/5)、CD15(0/5),LDHL肿瘤细胞表达CD30(1/1).CD30阳性率明显高于CD15(0.01＜P＜0.025).结论(1)NSHL中纤维组织增生形成结节,瘤细胞以陷窝细胞为主;MCHL中诊断型R-S细胞及其它变体细胞数量较多;LDHL以淋巴细胞减少为其特征.均以表达CD30为其特征.(2)在何杰金氏病鉴别判断中一定要应用免疫组化.CD30、CD45RO、Ki-67、CD20一般为一线抗体.  相似文献