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Novel distribution pattern of fibrinolytic components in rabbit tissues extract： a preliminary study
Lu XG Wu XG Xu XH Gong XB Zhou X Xu GB Zhu L Zhao XY 《Journal of Zhejiang University. Science. B》2007,8(8):570-574
Objective: The purpose of this work was to investigate the distribution pattern of fibrinolytic factors and their inhibitors in rabbit tissues. Methods: The components of the fibrinolytic system in extracts from a variety of rabbit tissues, including tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), plasminogen (Plg), plasmin (Pl) and α2 plasmin inhibitor (α2PI), were determined by colorimetric assay. Results: The tissue extracts in renal, small intestine, lung, brain and spleen demonstrated strong fibrinolytic function, in which high activity of tPA, Plg and Pl was manifested; whereas in skeletal muscle, tongue and stomach, higher activity of PAI-1 and α2PI showed obviously. Also excellent linear correlations were found between levels of tPA and PAI-1, Pl and α2PI, Plg and Pl. In related tissues, renal cortex and renal marrow showed distinctly higher activity of tPA and lower activity of PAI-1, with the levels of Plg and Pl in renal cortex being higher than those in renal marrow, where the α2PI level was higher than that in renal cortex. Similarly, the levels of tPA, Plg and Pl in small intestine were higher than those in large intestine, but with respect to PAI-1 and α2PI, the matter was reverse. In addition, the fibrinolytic activity in muscle tissue was lower, however, the levels of tPA, Plg, and Pl in cardiac muscle were obviously higher than those in skeletal muscles, and the levels of PAI-1 and α2PI were significantly lower than those in skeletal muscle. Conclusion: Our data demonstrate that a remarkable difference of the fibrinolytic patterns exists in rabbit tissues, which has probable profound significance in understanding the relationship between the function of haemostasis or thrombosis and the physiologic function in tissues. 相似文献
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金叶日本冬青组培增殖技术研究 总被引：1，自引：0，他引：1
朱志国 《安徽科技学院学报》2011,25(6)
以金叶日本冬青（Ilexcrenata）幼嫩茎段获得的无菌苗作外植体,通过在MS培养基中加入不同质量浓度的NAA和6-BA、选取茎段不同部位、设置液体培养基琼脂不同加量、不同pH值、不同光照强度等处理,分别对金叶日本冬青离体增殖培养进行研究,从而筛选增殖效果最佳的培养基配方。结果表明,1．0mg／L6-BA结合0．5mg／LNAA的增殖效果最佳;不同部位的外植体对增殖具有一定影响,试验发现采用茎尖培养,效果最佳;不同琼脂浓度、pH值及光照强度对其增殖均有一定影响,在生产中利用近似流体态的0．2％琼脂培养基效果较好;培养基pH值为6．0时效果最佳,芽数量多,苗生长健壮;在自然光培养下,芽增殖系数、苗高度和植株生长状况都优于相同光强的日光处理。 相似文献
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隐花色素基因(cryptochrome,简称cry)是一类生物钟基因,参与生物体昼夜节律调控。本实验克隆了二化螟2个隐花色素基因,分别命名为Cs-cry1和Cs-cry2(Genbank登录号分别为HG780135和KF977409),其分别包含1605 bp和2289 bp的开放阅读框,分别编码由534和762个氨基酸组成的蛋白;Cs-cry1与其它鳞翅目昆虫cry1的相似性较高,而Cs-cry2与其它鳞翅目昆虫cry2的相似性较高,这与系统进化分析结果相一致;半定量RT-PCR研究表明Cs-cry1和Cs-cry2基因在二化螟成虫触角、头、雄、腹、足和翅等不同组织中均有表达。实验结果为二化螟昼夜节律分子机制研究提供了理论依据。 相似文献
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It is a severe challenge to construct 3D scaf- folds which hold controllable pore structure and similar morphology of the natural extracellular matrix （ECM）. In this study, a compound technology is proposed by com- bining the 3D bioprinting and electrospinning process to fabricate 3D scaffolds, which are composed by orthogonal array gel microfibers in a grid-like arrangement and inter- calated by a nonwoven structure with randomly distributed polycaprolactone （PCL） nanofibers. Human adipose- derived stem cells （hASCs） are seeded on the hierarchical scaffold and cultured 21 d for in vitro study. The results of cells culturing show that the microfibers structure with controlled pores can allow the easy entrance of cells and the efficient diffusion of nutrients, and the nanofiber webs layered in the scaffold can significantly improve initial cell attachment and proliferation. The present work demon- strates that the hierarchical PCL/gel scaffolds consisting of controllable 3D architecture with interconnected pores and biomimetic nanofiber structures resembling the ECM can be designed and fabricated by the combination of 3D bioprinting and electrospinning to improve biological per- formance in tissue engineering applications. 相似文献
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阐述了在高职教育工学结合教学改革的大背景下《植物组织培养技术》课程教学改革的总体思路,详细介绍了“项目引领、任务驱动、教学做合一”的教学模式的具体做法及课程教学改革所取得的成果。 相似文献
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通过采用银针加艾灸的方法,对106名运动员急慢性软组织损伤的治疗效果进行观察,发现疗效优良者占80.19％,中占16.98％,差占2.83％。由于银针的针体较粗,导热性能良好,针灸后可改善受伤部位微循环,具明显镇痛作用,疗效较好。 相似文献
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Meng PQ Hou G Zhou GY Peng JP Dong Q Zheng S 《Journal of Zhejiang University. Science. B》2005,6(9):853-858
The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA （tissue microarray） paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method＇s relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology. 相似文献
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目的 :为了研究动脉粥样硬化性心脑血管疾病患者血浆脂蛋白 (a)水平与纤溶活性的关系 ,探讨脂蛋白 (a)促血栓形成和致动脉粥样硬化的机理。方法 :分别测定 4 2例心肌梗塞患者、4 2例心绞痛患者、4 4例脑梗塞患者及 38名健康对照者的脂蛋白 (a)浓度、组织型纤溶酶原激活剂 (tPA[lj1])及其抑制物 (PAI)活性 ,并分析其相关性。结果 :各组动脉粥样硬化性心脑血管疾病患者血浆脂蛋白 (a)浓度和PAI活性明显升高 ,tPA活性明显降低 ;各组脂蛋白 (a)与PAI活性均呈显著正相关。结论 :血浆高脂蛋白 (a)浓度是动脉粥样硬化性心脑血管疾病的主要危险因素之一 ,脂蛋白 (a)水平的升高与纤溶活性低下有关 相似文献