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1.
目的:观察模拟不同海拔高住低练的应激对大鼠红腓肠肌保护性蛋白热休克蛋白70 的影响。方法:将60 只SD 大鼠按体重随即分为6 组,高 住低练模型是采用在14.5%和12.7%两种低氧浓度模拟高原居住然后常氧以35 m/min×30 min(每3 天递增5 min)训练模型,共4 周。取大鼠红腓肠 肌进行RT- PCR 技术和免疫组织化学技术检测HSP70 的表达。结果:单纯训练、低氧暴露和低氧训练均能诱导HSP70 表达;与单纯训练组比较,单 纯低氧暴露和低氧训练组的HSP70 表达增加(P<0.05),其中低氧14.5%和低氧训练12.7%两组表达增加显著(P<0.01)。结论:低氧和训练或低氧训练均诱导热休克蛋白70 表达增加,低氧训练对热休克蛋白70 表达影响比单纯低氧暴露或训练的刺激方式更加明显。  相似文献   
2.
The aim of the present study was to examine the effects of viscoelastic properties of human tendon structures during stretch?–?shortening cycle exercise. The elongation of tendon and aponeurosis of the medial gastrocnemius muscle of 26 participants was measured by ultrasonography while they performed ramp isometric plantar flexion up to the voluntary maximum, followed by a ramp relaxation. The relationship between estimated muscle force and tendon elongation during the ascending phase was fitted to a linear regression, the slope of which was defined as stiffness. The percentage of the area within the muscle force?–?tendon elongation loop relative to the area beneath the curve during the ascending phase was defined as hysteresis. In addition, maximal voluntary concentric contractions at 2.09 and 3.14 rad?·?s?1 with and without prior eccentric contractions were performed. The difference in the concentric torque at equivalent joint angles with and without prior eccentric contractions (i.e. pre-stretch augmentation) was negatively correlated with stiffness (P <?0.05) and hysteresis (P <?0.05). Furthermore, there was a higher correlation between the pre-stretch augmentation and the viscoelastic properties index – that is, the sum of normalized score values of stiffness and hysteresis (P <?0.01) – than with either stiffness or hysteresis alone. The results of this study suggest that performance during stretch?–?shortening cycle exercise is significantly affected by the viscoelastic properties of the tendon structures.  相似文献   
3.
香菇多糖抗疲劳的实验研究   总被引:7,自引:0,他引:7  
研究了香菇多糖的抗疲劳作用.用离体实验测定香菇多糖对肌肉收缩时间的影响,在体实验测定肌糖原含量.通过差异与对照组相比较时,发现香菇多糖的抗疲劳作用并无显统计学意义.  相似文献   
4.
pH值对蟾蜍离体腓肠肌收缩能力的影响   总被引:4,自引:0,他引:4  
目的:观察pH值对赡蜍离体排肠收缩能力的影响。方法:采用D951生理实验教学系统,记录分析了置于PH值为6.2、6.4、6.6、6.8、7.0、7.2、7.4、7.6、7.8任氏液中的赡蜍离体腓肠肌(n=94)的单收缩曲线。结果:①赡蜍腓肠肌收缩能力最佳的pH值为6.8—7.2;②pH值在7.4—7.8时,肌肉在初期出现强直收缩,且pH值越高,强直收缩的持续时间越长;③随肌肉收缩的不断进行,舒张期时间逐渐延长,缩短期时间不变。结论:赡蜍腓肠肌在pH6.8—7.2之间收缩能力最佳,PH值过高或过低都将导致肌肉的收缩能力下降;肌肉舒张能力的降低是肌肉疲劳的主要特征之一。提示:为防止肌肉疲劳应提高人体对政的缓冲能力,但碱性物质的过量补充也可导致肌肉收缩能力下降,且易导致运动初期肌肉的强直性收缩。  相似文献   
5.
目的:通过实验研究探讨抗阻训练对老龄大鼠腓肠肌Ca2+及线粒体膜电位的影响。方法:研究对象为雄性SD大鼠,人工喂养18月龄进行8周抗阻训练干预,分别以无负重、30%、50%、70%最大负重进行训练,跑台放置坡度为35°,跑速15m/min,隔天抗阻训练。8周末检测腓肠肌胞浆Ca2+及线粒体膜电位,并与安静组比较。结果:30%、50%最大负重抗阻训练两组老龄大鼠腓肠肌Ca2+下降具有显著性意义(P0.05);无负重、30%、50%最大负重抗阻训练三组老龄大鼠线粒体膜电位下降有极显著性差异(P0.01)。结论:低、中等负重抗阻训练能降低衰老过程中腓肠肌胞浆Ca2+、提高线粒体膜电位,改善线粒体功能,从而预防、延迟衰老过程中线粒体功能障碍的发生。  相似文献   
6.
研究腓肠肌振动频谱分布规律,利用有限元软件ANSYS建立腓肠肌振动模型,计算腓肠肌提踵时振动的固有频率及振型,并使用专门的肌声检测系统测量10名大学男生负重提踵时的腓肠肌肌声信号。模拟结果显示,腓肠肌的振型随频率的增大存在一定的分布规律,实验测量表明振动的能量主要集中在超低频区。将模拟结果与实验数据进行对比分析,表明能量集中在超低频区的振型主要有4种。  相似文献   
7.
采用分组实验方法,观察长时间下坡跑对大鼠腓肠肌和比目鱼肌(离心收缩)肌动蛋白基因表达的影响,同时监测血清CK、LDH的变化。结果显示,C组大鼠腓肠肌及B组大鼠比目鱼肌肌动蛋白基因表达均高于对照组,血清CK、LDH也高于对照组。结果提示,一次长时间离心运动可造成骨骼肌的损伤,快肌更为明显;1周连续运动,机体可对其逐渐产生适应,但损伤的修复需要较长时间,尤其是收缩蛋白的修复。  相似文献   
8.
Abstract

The purpose of this study was to investigate changes in ankle joint stiffness and the associated changes in the gastrocnemius muscle and tendon due to static stretching. Seven healthy male participants lay supine with the hip and knee joints fully extended. The right ankle joint was rotated into dorsiflexion from a 30° plantar flexed position and the torque measured by a dynamometer. The ankle joint was maintained in a dorsiflexed position for 20 min (static stretching of the calf muscles). We performed surface electromyography of the medial and lateral gastrocnemii, the soleus, and the tibialis anterior of the right leg to confirm no muscle activity throughout static stretching and the passive test (passive dorsiflexion). During static stretching, the ankle joint angle and elongation of the gastrocnemius were recorded by goniometry and ultrasonography, respectively. Tendon elongation of the gastrocnemius was calculated based on the changes in the ankle joint angle and muscle elongation. In addition, the relationships between passive torques and ankle joint angles, and elongation of muscle and tendon, were examined before and after static stretching. The ankle dorsiflexion angle and tendon elongation increased significantly by 10 min after the onset of static stretching, while there was no further increase in muscle length. In addition, ankle dorsiflexion angle and tendon elongation at an identical passive torque (30 N · m) increased significantly (from 24±7° to 33±5° and from 17±2 mm to 22±1 mm, respectively) after static stretching. However, muscle elongation was unchanged. In conclusion, the current results suggest that an increase in the ankle joint dorsiflexion angle due to static stretching is attributable to a change in tendon not muscle stiffness.  相似文献   
9.
This study examined the effect of diamond deloading tape on medial gastrocnemius (MG) muscle behaviour during exercise in healthy adults (n = 27). A randomised cross-over trial assessed the effect of tape (no-tape, sham-tape and deload-tape) on ankle and MG fascicle kinematics during three heel raise-lower exercises [double leg (DL), single leg (SL) and loaded single leg (LSL)]. There was no effect of tape on standing fascicle length (FL) or pennation angle (PA), or ankle or knee joint angle. There was a significant effect of tape on ankle kinematics for all exercises. Both the deload-tape and sham-tape resulted in less ankle plantar flexion but had no effect on dorsiflexion. There was a significant effect of tape on FL change for the SL and LSL exercise. Compared to no-tape, the deload-tape resulted in less fascicle shortening during ankle plantar flexion, and more fascicle lengthening during ankle dorsiflexion. For the LSL exercise, deload-tape caused MG fascicles to operate at longer lengths, for a given joint angle. Diamond taping, with or without added tension, has only a small effect on ankle and MG fascicle kinematics during the heel raise-lower exercise. With the exception of the LSL exercise, both tape conditions resulted in similar changes in the FL-angle relations.  相似文献   
10.
目的:研究不同强度运动对SD大鼠肝、比目鱼肌和腓肠肌中雄激素受体(androgen receptor,AR)mRNA和蛋白表达的影响。方法:将SD大鼠随机分为对照组(C=10)、中强度训练组(M=10)、大强度训练组(L=10)、过度疲劳训练组(O=20),进行8周递增负荷跑台训练,每周训练6 d。在5周、8周训练结束后检测外周血Hb浓度、睾酮和皮质酮水平。8周训练结束后,将大鼠处死,取肝、比目鱼肌、腓肠肌,检测AR的mRNA和蛋白水平。结果:1)与安静对照组相比,中强度运动组大鼠的体重、Hb含量、睾酮/皮质酮(T/C)值无显著差异,而大强度及过度疲劳训练组大鼠的体重、Hb含量、血浆睾酮水平及T/C值均显著下降,且过度疲劳训练组下降更明显,表明各训练组大鼠的训练强度存在显著性差异;2)8周训练后,中强度训练组大鼠肝、比目鱼肌中AR的mRNA和蛋白水平显著升高,过度疲劳训练组则显著下降;3)8周训练后,中等和大强度运动不影响大鼠腓肠肌中AR的mRNA和蛋白水平,但过度疲劳训练组腓肠肌中AR的mRNA和蛋白水平显著升高。结论:1)中强度运动增加大鼠肝、比目鱼肌AR的mRNA和蛋白水平,过度疲劳运动抑制肝、比目鱼肌AR的mRNA和蛋白水平,而大强度运动对肝、比目鱼肌AR的mRNA和蛋白水平影响不大;2)中、大强度运动对腓肠肌AR的表达影响不大,过度疲劳运动促进腓肠肌AR的mRNA和蛋白表达,该作用可能与血浆中较低的雄激素水平有关。  相似文献   
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