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| 高效液相色谱串联质谱法测定蜂蜜中马来酸和水杨酸 | |
| 引用本文： | 周红霞,张凤,许蔚,杨雯筌,柳菡,徐锦忠.高效液相色谱串联质谱法测定蜂蜜中马来酸和水杨酸[J].南京晓庄学院学报,2010,26(3):57-60. |
| 作者姓名： | 周红霞 张凤 许蔚 杨雯筌 柳菡 徐锦忠 |
| 作者单位： | 1. 南京晓庄学院生物化工与环境工程学院,江苏,南京,211171 2. 南京晓庄学院生物化工与环境工程学院,江苏,南京,211171;江苏省出入境检验检疫局食品实验室,江苏,南京,210001 3. 江苏省出入境检验检疫局食品实验室,江苏,南京,210001 |
| 基金项目： | 国家十一五科技支撑计划项目No2006BAJ02A08-6) |
| 摘 要： | 建立了蜂蜜中的马来酸和水杨酸的高效液相色谱串联质谱的检测方法.采用VARIANC18-Ether色谱柱（150×2 mm,5μm）检测,以甲酸和甲醇为流动相,流速250μL/min.结果,在0.2mg.L-1—5.0 mg·L^-1范围内,马来酸和水杨酸浓度与峰面积呈良好的线性关系,（R^2〉0.9900）;在50μg·kg^-1、100μg·kg^-1、200μg·kg^-1三个添加水平下,马来酸和水杨酸的平均回收率范围为80%-130%;定量限为20μg·kg^-1.该方法简便、快速、灵敏、准确. |
| 关 键 词： | 高效液相色谱串联质谱法 蜂蜜 马来酸 水杨酸 |
Determination of Maleic Acid and Salicylic Acid in Honey by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| ZHOU Hong-xia,ZHANG Feng,XU-Wei,YANG Wen-quan,LIU-Han,XU Jin-zhong.Determination of Maleic Acid and Salicylic Acid in Honey by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Journal of Nanjing Xiaozhuang College,2010,26(3):57-60. | |
| Authors: | ZHOU Hong-xia ZHANG Feng XU-Wei YANG Wen-quan LIU-Han XU Jin-zhong |
| Institution: | 1.School of Biochemistry and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,Jiangsu;2.Food Laboratory,Jiangsu Import ＆ Export Inspection and Quarantine Bureau,Nanjing 210001,Jiangsu) |
| Abstract: | The method of High Performance Liquid Chromatography-Tandem Mass Spectrometry is adopted to determine maleic acid and salicylic acid in honey and chromatographic analysis is performed on a C18-Ether（150×2.0mm）column edelut with methanol and formic acid at the flow rate of 0.1ml/min.The calibration curve is linear at the range of 0.2—5.0 mg·l^-1 with good precision and accuracy（R^2〉0.99）.The average recoveries for the maleic acid and salicylic acid ranges from 80 to 130% in replicate sets of honey samples fortified with drug concentration of 50,100,200 μg·kg^-1.The limit of quantity is 20μg·kg^-1.This method is proved to be simple,rapid,sensitive and accurate. |
| Keywords: | High Performance Liquid Chromatography-tandem Mass Spectrometry honey maleic acid salicylic acid |
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