| Science Letters： A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen |
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| 作者姓名： | Zhou C Kang XD Chen Z |
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| 作者单位： | [1]Institute of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China [2]Experimental Animal Center, School of Medicine, Hangzhou Teacher＇s College, Hangzhou 310018, China |
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| 基金项目： | Project(No.39800135)supported by National Natural Science Foundation of China |
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| 摘 要： | It has been proposed that activation of Toll-like receptors (TLRs) plays crucial roles in the polarization of adaptive immune responses. A synthetic Toll-like receptor 2 (TLR2) ligand, Pam3CSK4, has been reported to modulate the balance of Thl/Tn2 responses. We evaluated the modulation effect of Pam3CSK4 on allergic immune response in a mouse rhinitis model sensitized to house dust mite allergen (HDM). Mice were sensitized and challenged with Dermatophagoides farinae allergen (Der f), and then the allergic mice were treated by Pam3CSK4. Nasal allergic symptoms and eosinophils were scored. Der f-specific cytokine responses were examined in the splenocytes and bronchoalveolar lavage fluid (BALF). Serum level of total IgE was also detected. After establishing a mouse allergic rhinitis model with HDM, we have showed that Pam3CSK4 treatment not only ameliorated the nasal allergic symptoms remarkably but also decreased the eosinophils and total inflammation cells in BALF significantly. Analysis of cytokine profile found that' IFN-γ released from either BALF or stimulated splenocytes increased markedly in Pam3CSK4-treated mice, while IL-13 decreased significantly. Moreover, serum level of total IgE was significantly lower in Pam3CSK4-treated mice than in the untreated. Thus, in an allergic rhinitis mouse model developed with HDM, Pam3CSK4 was shown to exhibit an antiallergic effect, indicating its potential application in allergic diseases.
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| 关 键 词： | 变应原 鼻疾病 免疫反应 医学免疫学 |
| 收稿时间： | 11 December 2007 |
| 修稿时间： | 4 February 2008 |
A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen |
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| Zhou C,Kang XD,Chen Z.A synthetic Toll-like receptor 2 ligand decreases allergic immune responses in a mouse rhinitis model sensitized to mite allergen[J].Journal of Zhejiang University Science,2008,9(4):279-285. |
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| Authors: | Zhou Cheng Kang Xiao-Dong Chen Zhi |
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| Institution: | (1) Institute of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China;(2) Experimental Animal Center, School of Medicine, Hangzhou Teacher’s College, Hangzhou, 310018, China |
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| Abstract: | It has been proposed that activation of Toll-like receptors (TLRs) plays crucial roles in the polarization of adaptive immune
responses. A synthetic Toll-like receptor 2 (TLR2) ligand, Pam3CSK4, has been reported to modulate the balance of Th1/Th2
responses. We evaluated the modulation effect of Pam3CSK4 on allergic immune response in a mouse rhinitis model sensitized
to house dust mite allergen (HDM). Mice were sensitized and challenged with Dermatophagoides farinae allergen (Der f), and then the allergic mice were treated by Pam3CSK4. Nasal allergic symptoms and eosinophils were scored.
Der f-specific cytokine responses were examined in the splenocytes and bronchoalveolar lavage fluid (BALF). Serum level of
total IgE was also detected. After establishing a mouse allergic rhinitis model with HDM, we have showed that Pam3CSK4 treatment
not only ameliorated the nasal allergic symptoms remarkably but also decreased the eosinophils and total inflammation cells
in BALF significantly. Analysis of cytokine profile found that IFN-γ released from either BALF or stimulated splenocytes increased
markedly in Pam3CSK4-treated mice, while IL-13 decreased significantly. Moreover, serum level of total IgE was significantly
lower in Pam3CSK4-treated mice than in the untreated. Thus, in an allergic rhinitis mouse model developed with HDM, Pam3CSK4
was shown to exhibit an antiallergic effect, indicating its potential application in allergic diseases.
Project (No. 39800135) supported by the National Natural Science Foundation of China |
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| Keywords: | Toll-like receptor 2 (TLR2) Ligand Allergic immune response Mite allergen |
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