Use of specific IgE to fractions of gynandropsis gynandra pollen inin vitro diagnostic test |
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| Authors: | G Suman Latha Vijaya Lakshmi H Surekha Rani K J R Murthy MD FCCP |
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| Institution: | (1) 10/1/1, Bhagwan Mahavir Hospital and Research Center, A.C. Guards, 500 004 Hyderabad, A.P., India |
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| Abstract: | The study was aimed at presence of specific IgE antibody levelsinvitro to the identified antigen. Based on positive skin test with Gynandropsis gynandra and elevated levels of total IgE (>325
IU/ml) 104 patients were selected. Healthy, asymptomatic individuals (25) with low total IgE (<325 IU/ml) were included as
controls. The mean OD values by ELISA for specific IgE were 0.67±0.21, 0.57±0.18 and 0.56±0.18 with whole pollen antigen,
46-37 kD fraction and 36-32 kD fraction, respectively. The specificity and sensitivity between skin test positivity with whole
pollen antigen verses fraction with mol.wt 46-37 kD was 90% and 90% and for fraction with mol.wt 36-32 kD was found to be
81.1% and 89.4%. The clusters with molecular weights 46-37 kD and 36-32 kD may be useful inin vitro diagnostic test. Fractions within these clusters need to be identified for a higher specificity. |
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| Keywords: | ELISA Gynandropsis gynandra IgE Skin test Specificity Sensitivity |
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