| Hydrolysis of Raw Corn Starch Granules by Glucoamylase and Product Inhibition During the Hydrolysis |
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| 作者姓名： | 王金鹏 曾爱武 刘振 袁希钢 吴少敏 |
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| 作者单位： | [1]StateKeyLaboratoryofChemicalEngineering,.＇SchoolofChemicalEngineering,TianjinUniversity,Tianjin300072,China [2]StateKeyLaboratoryofChemicalEngineering,.＇SchoolofChemicalEngineering,TianjinUniversity,Tianjin300072,China |
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| 摘 要： | Raw corn starch granules were hydrolysized by glucoamylase in a chemostat. The hydrolysis of three different-sized granules shows that smaller granules undergo more hydrolyzation than larger ones. After 78 h, 97% of the granules was hydrolysized with diameter between 0.15 mm and 0.3 mm at 50℃. When corn starch concentration increased from 100 g/L to 250 g/L, the amount of reducing sugar produced was proportional to the initial substrate concentration and no substrate inhibition phenomenon appeared. In order to study the product inhibition exactly, the product from hydrolysis reaction itself was added into the hydrolysis system at the beginning of starch hydrolysis.Product inhibition with different quantities of product added were studied in the initial several hours,during which period enzyme inactivation could be neglected and product inhibition could be studied separately. The experiments indicate that product inhibition happens when the additional quantity exceeds 9.56 g/L.
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| 关 键 词： | 水解 淀粉颗粒 葡糖淀粉酶 恒化器 生产抑制 |
Hydrolysis of Raw Corn Starch Granules by Glucoamylase and Product Inhibition During the Hydrolysis |
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| WANG Jinpeng,ZENG Aiwu,LIU Zhen,Yuan Xigang,Wu Shaomin.Hydrolysis of Raw Corn Starch Granules by Glucoamylase and Product Inhibition During the Hydrolysis[J].Transactions of Tianjin University,2005,11(3):199-203. |
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| Authors: | WANG Jinpeng ZENG Aiwu LIU Zhen Yuan Xigang Wu Shaomin |
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| Institution: | State Key Laboratory of Chemical Engineering, School of Chemical Engineering, Tianjin University, Tianjin 300072, China |
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| Abstract: | Raw corn starch granules were hydrolysized by glucoamylase in a chemostat. The hydro-lysis of three different-sized granules shows that smaller granules undergo more hydrolyzation than larger ones. After 78 h, 97% of the granules was hydrolysized with diameter between 0.15 mm and 0.3 mm at 50 ℃. When corn starch concentration increased from 100 g/L to 250 g/L, the amount of reducing sugar produced was proportional to the initial substrate concentration and no substrate inhibition phenomenon appeared. In order to study the product inhibition exactly, the product from hydrolysis reaction itself was added into the hydrolysis system at the beginning of starch hydrolysis. Product inhibition with different quantities of product added were studied in the initial several hours, during which period enzyme inactivation could be neglected and product inhibition could be studied separately. The experiments indicate that product inhibition happens when the additional quantity exceeds 9.56 g/L. |
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| Keywords: | hydrolysis starch granules glucoamylase product inhibition |
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