Bioresistive identification of heat shock protein 90 |
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| Authors: | Arvind Chandrasekaran Shanmugasundaram Pakkiriswami Jian Liang You Ashwin Acharya Muthukumaran Packirisamy and Denis Maxwell |
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| Institution: | 1Optical Microsystems Laboratory, Department of Mechanical Engineering,Concordia University, Montreal, Quebec H3G2W1, Canada;2Department of Biology, University of Western Ontario, London, Ontario N6A 5B7, Canada |
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| Abstract: | 90 kDa heat shock protein (HSP90) is a ubiquitous molecular chaperone and is one of the abundant proteins present in a cell under normal and stressed conditions. The adenosine triphosphate (ATP) binding region of HSP90 is currently under a great degree of study because of the interest of its role in cancer and protein maintenance; the binding of ATP to HSP90 induces a large conformational change in the protein as a result of the activity of different types of stressors within the cells. In the present paper, a simple microfluidic biosensor is proposed for the characterization of ATP-HSP90 interactions through the principle of bioresistive variation. The experimental results prove that the present biosensor system is highly suitable for the detection of heat shock proteins present in a real-time biological sample, which is very useful for in-situ biomedical applications and rapid pathogenic detections. |
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