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| 流式细胞术分析五味子乙素促进阿霉素内化的实验研究 | |
| 引用本文： | 孙德一,张铎,王海杰,李妍.流式细胞术分析五味子乙素促进阿霉素内化的实验研究[J].中国科技信息,2012(7):151-152. |
| 作者姓名： | 孙德一 张铎 王海杰 李妍 |
| 作者单位： | 1. 延边大学医学部药学院,延吉,133002 2. 吉林医药学院免疫学与病原学教研室,吉林,132013 3. 吉林医药学院药学院,吉林,132013 |
| 基金项目： | 吉林省教育厅十二五科研基金（编号2011420） |
| 摘 要： | 目的]建立流式细胞术分析肿瘤细胞内阿霉素分布的方法,并研究低浓度五味子乙素对K562细胞内化阿霉素的影响。方法]体外培养猪内皮细胞(pEC),以20μmol/L阿霉素处理2、4和6小时;或加入不同浓度阿霉素(0、10、20、40、80和100μmol/L)处理4小时。培养K562细胞,以5μmol/L阿霉素处理2、4和6小时;或加入不同浓度阿霉素(0、2.5、5、10和20μmol/L)处理4小时。采用不同浓度(0、25、50和100μmol/L)五味子乙素(Sch B)与阿霉素联合处理K562细胞4小时。收集细胞,采用流式细胞术分析阿霉素的特异荧光。结果]固定浓度处理pEC(20μmol/L)和K562(5μmol/L)后检测,发现细胞内荧光强度随时间延长而增加,两种细胞4小时组荧光强度分别达到6小时组的96.93%和95.23%/。在直方图上,阴性和阳性细胞群界限分明。阿霉素(2.5、5和10μmol/L)单独处理细胞的荧光强度分别为26.78±3.34、64.70±6.24和118.35±9.67;添加25μmol/L Sch B实验后荧光强度增加到43.45±4.25、103.74±7.36和146.69±8.32,Sch B显著增加了K562细胞内阿霉素的分布(p<0.05)。结论]建立的方案可快速测定细胞内的阿霉素分布;低浓度Sch B促进肿瘤细胞内化阿霉素。 |
| 关 键 词： | 流式细胞术 阿霉素 五味子乙素 |
Analyzing the promotion of internalization of adriamycin by schisandrin B through flow cytometry |
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| Sun Deyi , Zhang Duo , wang Haijie , Li Yan.Analyzing the promotion of internalization of adriamycin by schisandrin B through flow cytometry[J].CHINA SCIENCE AND TECHNOLOGY INFORMATION,2012(7):151-152. | |
| Authors: | Sun Deyi Zhang Duo wang Haijie Li Yan |
| Institution: | 1.The Department of Pharmacy in The Medical College of Yanbian Univercity,Yanji,133002 2.The Department of Immunology and Etiology of Jilin Medical College,Jilin,132013 3.The Department of Pharmacy of Jilin Medical College,Jilin,132013 |
| Abstract: | Objective: To establish a method to analyze content of adriamycin in tumor cells by flow cytometry(FCM) and to analyze the effect of internalization of adriamycin into tumor cells promoted by schisandrin B.Method: Pig endothelia cells(pEC) were cultured with or without 20μmol/L adriamycin for indicated time(2,4 and 6 hours),or in the presence of different concentration(0,10,20,40 and 80μmol/L) of adriamycin for 4 hours.Human erythroleukemia cells K562 were cultured with or without 5μmol/L adriamycin for indicated time(2,4 and 6 hours),or in the presence of different concentration(0,2.5,5,10 and 20μmol/L) of adriamycin for 4 hours.K562 cells were treated by different concentration of adriamycin in the presence of schisandrin B(0,25,50 and 100 5μmol/L).The specific red fluorescence of adriamycin in those cells were detected by FCM.Result: The fluorescence in pEC and K562 cells treated by determined concentration of adriamycin(10μmol/L or 5μmol/L) increased time-dependently.Compared with cells treated for 6 hours,cells treated for 4 hours presented almost 96.93% and 95.23 of fluorescence.Fluorescence positive and negative cells were distinct well in the histograms.The fluorescence in K562 cells treated by adriamycin(2,5 and10 5μmol/L) along were 26.78±3.34、64.70±6.24 and 118.35±9.67.The addition of 25μmol/L Sch B promoted the internalization of adriamycin to 43.45±4.25、103.74±7.3 and 146.69±8.32(p<0.05).Conclusion: The method to analyze content of adriamycin in tumor cells by FCM was established.It was confirmed that lower concentration of Sch B could promoted the internalization of adriamycin. |
| Keywords: | flow cytometry adriamycin schisandrin B |
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