| 用包埋脱水法冰冻保存两种硅藻--预培养对包埋脱水法冰冻保存两种硅藻存活率的影响 |
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| 引用本文： | 李莹,李刚.用包埋脱水法冰冻保存两种硅藻--预培养对包埋脱水法冰冻保存两种硅藻存活率的影响[J].鞍山师范学院学报,2005,7(6):68-70. |
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| 作者姓名： | 李莹 李刚 |
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| 作者单位： | 1. 辽阳职业技术学院,辽宁,辽阳,111004
2. 鞍山师范学院,教育科学与技术学院,辽宁,鞍山,114005 |
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| 摘 要： | 采用包埋脱水法冰冻保存牟氏角刺藻（Chaetoceros muelleri）、小新月菱形藻（Nitzschia closterium minutissimai），探讨了预培养对两种硅藻存活率的影响．结果表明，用0．6M的蔗糖预培养时，牟氏角刺藻冰冻后的存活率有提高，最大可提高7．2％．但预培养对小新月菱形藻的存活率没有明显提高．
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| 关 键 词： | 牟氏角刺藻 小新月菱形藻 包埋脱水法 冰冻（超低温）保存 预培养 |
| 文章编号： | 1008-2441（2005）06-0068-03 |
| 收稿时间： | 2005-04-11 |
| 修稿时间： | 2005年4月11日 |
Cryopreservation of Two Diatoms by Encapsulation-dehydration--How Does the Pre-culture Influence on Algal Viability After Cryopreservation |
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| LI Ying,LI Gang.Cryopreservation of Two Diatoms by Encapsulation-dehydration--How Does the Pre-culture Influence on Algal Viability After Cryopreservation[J].Journal of Anshan Teachers College,2005,7(6):68-70. |
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| Authors: | LI Ying LI Gang |
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| Abstract: | Based on the preservation of the Chaetoceros muelleri and Nitzschia closterium minutissimai by encapsulation-dehydration,the influences of pre-culture on the two diatoms' algal viabilities are discussed in this paper.The experiment shows that the algal viability of Chaetoceros muelleri increases after cryopreservation when it was pre-cultured with 0.6 M of sucrose,the rate of it can reach 7.2% at most.In term of Nitzschia closterium minutissimai,no obvious clue to show any influence. |
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| Keywords: | Chaetoceros m uelleri Nitzschia closterium minutissimal Encapsulation-dehydration Cryopreservation Pre-culture |
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