| 细胞外钙离子对人成骨样细胞 MG-63分化的影响 |
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| 引用本文： | 郑家强,杨建虹.细胞外钙离子对人成骨样细胞 MG-63分化的影响[J].中国科学院研究生院学报,2011,28(1):57-64. |
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| 作者姓名： | 郑家强 杨建虹 |
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| 作者单位： | 中国科学院研究生院,北京,100049 |
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| 基金项目： | 中国科学院研究生院启动基金(055101FM03)资助 |
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| 摘 要： | 研究不同浓度钙离子对人成骨样细胞MG-63分化的影响.结果表明, 5.0mmol/L Ca2+促进 CaSR、ALP、OC、Runx 2、IGF-1和BMP2 mRNA表达,而加入CaSR和ERK抑制剂后,则下调 ALP、OC、Runx 2、IGF-1和BMP2 mRNA表达.不同浓度Ca2+处理后,磷酸化-ERK(p-ERK)蛋白表达明显增加.因此,5.0mmol/L Ca2+通过上调CaSR基因的表达,激活ERK通路,促进MG-63成骨样细胞的分化.
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| 关 键 词： | 钙离子 分化 成骨细胞 CaSR ERK |
| 收稿时间： | 2010-04-29 |
| 修稿时间： | 2010-05-07 |
Effect of extracellular Ca2+ on osteoblast differentiation in MG-63 cells |
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| ZHENG Jia-Qiang,YANG Jian-Hong.Effect of extracellular Ca2+ on osteoblast differentiation in MG-63 cells[J].Journal of the Graduate School of the Chinese Academy of Sciences,2011,28(1):57-64. |
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| Authors: | ZHENG Jia-Qiang YANG Jian-Hong |
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| Institution: | Graduate University, Chinese Academy of Sciences, Beijing 100049,China |
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| Abstract: | In the present study we have examined effects of extracellular Ca2+ on osteoblast differentiation in MG-63 cells.Theresults showed that5.0mmol/L Ca2+ up-regulated the expression of CaSR , OC、ALP、Runx 2、IGF-1 ,and BMP 2 . However,these indicators decreased after treatment with blocking agents. In addition,5.0 and 10.0mmol/L Ca2+ increased phospho-ERK expression, but did not affect ERK.Therefore 5.0mmol/L Ca2+ up-regulated CaSR and then promoted osteoblast differentiation through ERK pathway in MG-63 cells. |
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| Keywords: | Ca2+ differentiation osteoblast CaSR ERK |
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