| 运动疲劳诱导大鼠纹状体c-jun蛋白表达图谱变化的研究 |
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| 引用本文： | 侯莉娟,刘晓莉,乔德才.运动疲劳诱导大鼠纹状体c-jun蛋白表达图谱变化的研究[J].北京体育大学学报,2010(3). |
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| 作者姓名： | 侯莉娟 刘晓莉 乔德才 |
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| 作者单位： | 北京师范大学体育与运动学院; |
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| 基金项目： | 国家自然科学基金资助项目(No.30771050;30971416) |
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| 摘 要： | 目的:通过绘制运动疲劳后c-jun蛋白在纹状体神经核团中的表达图谱,寻找运动疲劳后纹状体神经元应激敏感区,探讨其在运动疲劳中枢调控中的作用。方法:雄性SD大鼠随机分为对照组和实验组,采用免疫组化法观察十天递增负荷游泳运动疲劳后即刻、12 h和24 h纹状体喙侧层面、中间层面、近尾侧层面和尾侧层面c-jun蛋白的表达。结果:纹状体中间层面的背外侧区和腹外侧区中,12 h和24 h阳性细胞面密度值显著高于即刻组(P<0.05);在近尾侧层面,阳性细胞总体表达上调,12 h和24 h四个分区中阳性细胞光密度均显著高于对照组(P<0.05,P<0.01),且12H表达最强;在尾侧层面中,阳性细胞表达不规则,仅12 h腹外侧区表达较对照组显著增强(P<0.01)。结论:运动疲劳诱导纹状体中间及近尾侧层面背外侧和腹外侧区域神经元即刻早期基因c-jun蛋白表达增强,此区域与运动疲劳后高频及爆发式放电神经元集中的区域重叠,与纹状体接受黑质多巴胺能神经元投射区域一致,说明多巴胺系统在运动疲劳后纹状体神经元电变化中起重要的调节作。
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| 关 键 词： | 运动疲劳 纹状体 即刻早期基因 c-jun蛋白 |
Influence of Exercise-induced Fatigue on the Expression of Immediate Early Gene in Rats' Striatum |
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| HOU Li-juan,LIU Xiao-li,QIAO De-cai.Influence of Exercise-induced Fatigue on the Expression of Immediate Early Gene in Rats' Striatum[J].Journal of Beijing Sport University,2010(3). |
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| Authors: | HOU Li-juan LIU Xiao-li QIAO De-cai |
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| Institution: | Physical Education and Sports College;Beijing Normal University;Beijing 100875;China |
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| Abstract: | Objective: to draw the c-jun protein expression figure after exercise-induced fatigue in order to find out the sensitivity area in striatum,and discuss its role of striatum in central regulation.Methods: Ten-day load increasing swimming exercise protocol is used to set up exercise fatigue animal model.SABC method is used to investigate the c-jun protein expression at different time in different area in striatum.Results: At the beak part,OD of c-jun expression cells in at dorsal part is significantly higher ... |
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| Keywords: | exercise-induced fatigue striatum immediate early gene c-jun protein |
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