| 肝中丁丙诺啡提取方法研究 |
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| 引用本文： | 贺江南,刘冬娴.肝中丁丙诺啡提取方法研究[J].湖南科技学院学报,2007,28(4):107-109. |
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| 作者姓名： | 贺江南 刘冬娴 |
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| 作者单位： | 湖南公安高等专科学校,刑事技术系,湖南,长沙,410138 |
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| 基金项目： | 公安部科研项目公科研[2004]163号 |
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| 摘 要： | 采用液相萃取法、固相萃取法提取肝中丁丙诺啡,考察不同提取剂、pH值等因素对萃取率的影响。肝匀浆中添加丁丙诺啡,液相萃取时调pH至7用三氯甲烷提取,固相萃取时调pH至10.8用401有机担体吸附、用三氯甲烷洗脱。提取液挥干后,以苯为溶剂,加入N,O-双（三甲基硅烷基）三氟乙酰胺衍生化,用GC/NPD法进行检测。结果表明,液相萃取肝中丁丙诺啡提取率达82.51%,固相萃取肝中丁丙诺啡提取率可达86.35%。这两种提取方法提取率均较高,可用于肝中丁丙诺啡的提取。
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| 关 键 词： | 丁丙诺啡 液相萃取 固相萃取 气相色谱 肝 |
| 文章编号： | 1673-2219（2007）04-0107-03 |
| 收稿时间： | 2006-12-11 |
| 修稿时间： | 2006年12月11 |
Study on Extraction Methods of Buprenorphine in Liver |
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| HE Jiang-nan,LIU Dong-xian.Study on Extraction Methods of Buprenorphine in Liver[J].Journal of Hunan University of Science and Engineering,2007,28(4):107-109. |
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| Authors: | HE Jiang-nan LIU Dong-xian |
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| Abstract: | To investigate the influence of solution, pH, adsorbents and so on, on the efficiency of extracting buprenorphine in liver. Buprenorphine was extracted with cholroform at pH7 or extracted by organic support 401 at pH10.8, then eluted with chloroform. After derivatized with N,O-bis(trimethylsilyl)-trifluoroacetamide, buprenorphine derivative was determined by GC-NPD. The recoveries of the two methods were 82.51% and 86.35% respectively. The two methods are simple and accurate methods for the separation of buprenorphine from liver. |
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| Keywords: | buprenorphine solid-phase extraction solution-phase extraction gas chromatography liver |
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